公开(公告)号：EP2954905A1

公开(公告)日：2015-12-16

申请号：EP13868860.1

申请日：2013-12-26

申请人： Nakajima, Toshihiro

发明人： NAKAJIMA, Toshihiro ,  FUJITA, Hidetoshi ,  ARATANI, Satoko ,  YAGISHITA, Naoko

IPC分类号： A61K45/00 ,  A61P3/04 ,  A61P43/00 ,  C12Q1/68 ,  G01N33/15 ,  G01N33/50

CPC分类号： G01N33/573 ,  C12Q1/6883 ,  C12Q2600/136 ,  C12Q2600/158 ,  G01N33/5044 ,  G01N33/6875 ,  G01N2333/70567 ,  G01N2333/9015 ,  G01N2440/36 ,  G01N2500/04 ,  G01N2500/10

摘要： [Problem]
To provide a mitochondria-function regulator effective for treatment or prevention of obesity, and a screening method therefore.
[Solution]
The mitochondria-function regulator of the present invention contains, as an active ingredient, a PGC-1β - protein function regulator synoviolin. The screening method of the present invention includes a step for causing a test substance to act on an adipose tissue cell or an individual animal, and measuring or detecting one or more of the following in the adipose tissue cell: (1) expression level of synoviolin; (2) a bond between synoviolin and PGC-1β protein; and (3) the ubiquitination of the PGC-1β protein by synoviolin.

公开(公告)号：EP2638394B1

公开(公告)日：2017-05-10

申请号：EP11839316.4

申请日：2011-11-14

申请人： Northern Sydney Local Health District

发明人： MARSH, Deborah Joy ,  HAHN, Michael Anthony

IPC分类号： G01N33/53 ,  C07K16/18 ,  C07K16/30 ,  G01N33/574

CPC分类号： G01N33/6893 ,  G01N33/57484 ,  G01N2440/36 ,  G01N2500/02

摘要： The present disclosure relates generally to the field of cancer diagnosis. More specifically, the present disclosure relates to the identification and use of monoubiquitination of histone 2B as a biomarker for the diagnosis and prognosis of cancer including, but not limited to, parathyroid cancer. The present disclosure also relates to the identification of binding between CDC73 and RNF20, and the use of CDC73 and RNF20 in an assay for screening for an agent that modulates monoubiquitination of a histone protein.

公开(公告)号：EP2968987A1

公开(公告)日：2016-01-20

申请号：EP14763405.9

申请日：2014-03-14

申请人： Vertex Pharmaceuticals Inc. ,  The University of North Carolina at Chapel Hill

发明人： VAN GOOR, Fredrick F. ,  HOFFMAN, Beth Jennifer ,  DELA ROSA, Oxana Adolfovna ,  CYR, Douglas

IPC分类号： A61P11/00 ,  C12Q1/00

CPC分类号： A61K31/443 ,  A61K31/407 ,  A61K31/47 ,  A61K31/69 ,  A61K45/06 ,  C12Q1/34 ,  G01N33/5023 ,  G01N33/5035 ,  G01N33/5041 ,  G01N33/6872 ,  G01N2333/914 ,  G01N2440/36 ,  G01N2500/02 ,  G01N2800/382 ,  A61K2300/00

摘要： The present disclosure provides methods for treating Cystic Fibrosis in a subject by administering to the subject a corrector agent capable of acting through MSD1 during the biosynthesis of CFTR protein. The disclosure also provides methods of screening for new corrector agents capable of acting through MSD1 during the biosynthesis of a CFTR protein.

摘要翻译： 本公开提供了通过向受试者施用能够通过MSD1在CFTR蛋白的生物合成中起作用的校正剂来治疗受试者的囊性纤维化的方法。 本公开还提供了在CFTR蛋白的生物合成过程中筛选能够通过MSD1作用的新校正剂的方法。

公开(公告)号：EP2736516A4

公开(公告)日：2014-12-31

申请号：EP12811596

申请日：2012-07-13

申请人： ICAHN SCHOOL MED MOUNT SINAI

发明人： HAJJAR ROGER JOSEPH ,  KHO CHANG WON ,  LEE AH YOUNG

IPC分类号： A61K31/7088 ,  C12N15/11 ,  C12N15/113

CPC分类号： A61K38/50 ,  A61K31/7088 ,  A61K38/1709 ,  A61K48/0066 ,  C12N7/00 ,  C12N15/86 ,  C12N2750/14143 ,  C12Q1/6883 ,  C12Q2600/136 ,  C12Q2600/158 ,  C12Y305/00 ,  G01N33/573 ,  G01N2333/914 ,  G01N2440/36 ,  G01N2800/325

公开(公告)号：EP2758541A1

公开(公告)日：2014-07-30

申请号：EP12766385.4

申请日：2012-09-24

申请人： Medical Research Council

发明人： MEVISSEN, Tycho E. T. ,  HOSPENTHAL, Manuela K. ,  KOMANDER, David

IPC分类号： C12Q1/34 ,  G01N33/68 ,  C12Q1/25 ,  G01N33/50

CPC分类号： C12Q1/37 ,  C12N9/485 ,  G01N33/6842 ,  G01N2440/36

摘要： There is described a method for analyzing ubiquitin polymers using linkage-specific deubiquitinase enzymes. Novel specificities of deubiquitinase enzymes are also provided.

摘要翻译： 描述了使用连接特异性去泛素化酶分析泛蛋白聚合物的方法。 还提供了去泛素化酶的新特异性。

公开(公告)号：EP2736516A1

公开(公告)日：2014-06-04

申请号：EP12811596.1

申请日：2012-07-13

申请人： Icahn School of Medicine at Mount Sinai

发明人： HAJJAR, Roger, Joseph ,  KHO, Chang, Won ,  LEE, Ah, Young

IPC分类号： A61K31/7088 ,  C12N15/11 ,  C12N15/113

CPC分类号： A61K38/50 ,  A61K31/7088 ,  A61K38/1709 ,  A61K48/0066 ,  C12N7/00 ,  C12N15/86 ,  C12N2750/14143 ,  C12Q1/6883 ,  C12Q2600/136 ,  C12Q2600/158 ,  C12Y305/00 ,  G01N33/573 ,  G01N2333/914 ,  G01N2440/36 ,  G01N2800/325

摘要： Methods for treating cardiovascular disease, and in particular heart failure, are provided comprising administering a therapeutically effective amount of a modulator of SERCA2a post-translation modification such as SUMOylation or acetylation. Also provided are methods of treating cardiovascular disease by inhibiting SERCA2a degradation. Further provided are methods of diagnosing a propensity to develop heart failure comprising determining if a SERCA2a mutant is present or determining the level of expression of SUMOl in cardiomyocytes. The disclosure also provides methods of screening for therapeutics that modulate the post-translational modification of SERCA2a, such as by modulating post-translational SUMOylation and/or acetylation.

摘要翻译： 提供了治疗心血管疾病，特别是心力衰竭的方法，包括施用治疗有效量的SERCA2a翻译后修饰调节剂，例如SUMO化或乙酰化。 还提供了通过抑制SERCA2a降解来治疗心血管疾病的方法。 进一步提供了诊断发展为心力衰竭倾向的方法，包括确定SERCA2a突变体是否存在或确定SUMO1在心肌细胞中的表达水平。 本公开还提供筛选调节SERCA2a的翻译后修饰的治疗剂的方法，例如通过调节翻译后SUMO化和/或乙酰化。

公开(公告)号：EP2558496A4

公开(公告)日：2014-01-01

申请号：EP11769579

申请日：2011-04-14

申请人： GENENTECH INC

发明人： DIXIT VISHVA ,  KELLEY ROBERT F ,  MATSUMOTO MARISSA L

IPC分类号： C07K16/18 ,  A61K39/395

CPC分类号： C07K16/18 ,  C07K14/4702 ,  C07K2317/24 ,  C07K2317/30 ,  C07K2317/55 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/92 ,  C12N15/1037 ,  G01N33/68 ,  G01N2333/4703 ,  G01N2440/36

摘要： The invention provides anti-polyubiquitin antibodies and methods of using the same.

公开(公告)号：EP2580344A1

公开(公告)日：2013-04-17

申请号：EP11729135.1

申请日：2011-06-14

申请人： Medical Research Council

发明人： KOMANDER, David ,  YE, Yu

IPC分类号： C12Q1/37

CPC分类号： C07K17/00 ,  C12Q1/37 ,  G01N2440/36 ,  G01N2500/04

摘要： There is described a substrate for measuring the activity of a deubiquitinating enzyme (DUB), comprising a diubiquitin molecule, wherein an ubiquitin monomer is labeled with a fluorescent label, as well as an assay for DUB enzymes using such substrates.

摘要翻译： 描述了用于测量脱遍在蛋白化酶（DUB）活性的底物，其包含二泛素分子，其中泛素单体用荧光标记物标记，以及使用这种底物的DUB酶测定法。

公开(公告)号：EP2341345A1

公开(公告)日：2011-07-06

申请号：EP10015839.3

申请日：2005-08-18

申请人： Odyssey Thera, Inc.

发明人： Westwick, John ,  Yu, Helen ,  MacDonald, Marnie L.

IPC分类号： G01N33/50 ,  C12Q1/02

CPC分类号： C12Q1/025 ,  G01N33/5023 ,  G01N33/5035 ,  G01N33/5041 ,  G01N33/5097 ,  G01N2440/00 ,  G01N2440/10 ,  G01N2440/12 ,  G01N2440/36 ,  G01N2440/38 ,  G01N2500/10

摘要： The instant invention provides a method for establishing safety profiles for chemical compounds, as well as pharmacological profiling said method comprising (A) testing the effects of said chemical compounds on the amount and/or post-translational modifications of two or more macromolecules in intact cells; (B) constructing a pharmacological profile based on the results of said tests; and (C) comparing said profile to the profile(s) of drugs with established safety characteristics. Additionally, the invention is also directed to a composition comprising an assay panel, said panel comprising at least one high-content assay for the amount and/or post-translational modification of a protein and at least one high-content assay for the amount and/or subcellular location of a protein-protein interaction.

摘要翻译： 本发明提供了一种用于建立化学化合物的安全性的方法以及药理学分析，所述方法包括（A）测试所述化合物对完整细胞中两种或更多种大分子的量和/或翻译后修饰的影响 ; （B）基于所述测试的结果构建药理学特征; 和（C）将所述概况与具有确定的安全特征的药物的概况进行比较。 此外，本发明还涉及包含测定面板的组合物，所述面板包含至少一种用于蛋白质的量和/或翻译后修饰的高含量测定，以及至少一种高含量测定法，其量为和 /或蛋白质 - 蛋白质相互作用的亚细胞位置。