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1308 条记录 (耗时 0.019 秒)

    METHOD FOR PRODUCING PLURIPOTENT STEM CELLS CAPABLE OF DIFFERENTIATING INTO SPECIFIC CELLS, AND APPLICATION THEREOF
    23.
    发明公开
    METHOD FOR PRODUCING PLURIPOTENT STEM CELLS CAPABLE OF DIFFERENTIATING INTO SPECIFIC CELLS, AND APPLICATION THEREOF 审中-公开

    公开(公告)号：EP4092107A1

    公开(公告)日：2022-11-23

    申请号：EP21741077.8

    申请日：2021-01-15

    申请人： FUJIFILM Corporation

    发明人： MURAKAMI Yuta ,  NAGASE Masaya ,  MAEDA Kiyohiro ,  SHIRAISHI Yasushi ,  KOCHI Masahiro

    IPC分类号： C12N5/071 ,  C12N5/0735 ,  C12N5/077 ,  C12N5/10 ,  C12Q1/06

    摘要： An object of the present invention is to provide a method of producing a pluripotent stem cell capable of differentiating into a specific cell. According to the present invention, there is provided a method for producing a pluripotent stem cell capable of differentiating into a specific cell, the method including (i) a step of measuring, in a pluripotent stem cell as a sample, a phenotype associated with induction of epithelial-mesenchymal transition before differentiation induction; and (ii) a step of acquiring a pluripotent stem cell capable of differentiating into a specific cell using the measured phenotype as an indicator. According to the present invention, there are further provided a method of selecting a pluripotent stem cell capable of differentiating into a specific cell; a pluripotent stem cell capable of differentiating into a specific cell; a production method of a differentiated cell; a differentiated cell; and a method for quality evaluation of a pluripotent stem cell.

    COMPOSITIONS AND METHODS FOR PRODUCING GENETICALLY MODIFIED ANIMALS
    25.
    发明公开
    COMPOSITIONS AND METHODS FOR PRODUCING GENETICALLY MODIFIED ANIMALS 审中-公开

    公开(公告)号：EP4043575A1

    公开(公告)日：2022-08-17

    申请号：EP21177140.7

    申请日：2014-11-07

    申请人： Ozgene Holdings Pty Ltd

    发明人： KOENTGEN, Frank

    IPC分类号： C12N15/877 ,  C12N5/10 ,  A01K67/027 ,  C12N15/85 ,  C12N5/0735

    摘要： Methods, compositions and non-human animals and parts thereof are disclosed for improving germ line transmission of genetic modifications. More particular, the present invention discloses methods and compositions for producing non-human embryos with a disrupted or disruptable fertility gene, which can be used as hosts for the development of donor pluripotent cells, including genetically modified donor pluripotent cells, into germ cells and gametes. Also disclosed are methods and compositions for producing from such embryos chimeric non-human animals with a disrupted fertility gene and for breeding the chimeric non-human animals with cognate non-human animals that comprise a fertility gene that lacks a disruption to produce non-human animals having substantially all gametes and/or germ cells derived from the donor pluripotent cells. The present invention also discloses non-human gametes, germ cells, embryos and animals for use in the subject methods.

    CELL CULTURE APPARATUS AND CELL CULTURE METHOD
    26.
    发明公开
    CELL CULTURE APPARATUS AND CELL CULTURE METHOD 审中-公开

    公开(公告)号：EP4039792A1

    公开(公告)日：2022-08-10

    申请号：EP22165428.8

    申请日：2018-01-19

    申请人： FUJIFILM Corporation

    发明人： Takayama, Hidetoshi ,  Goto, Shun

    IPC分类号： C12M3/02 ,  C12M3/06 ,  C12M1/36 ,  C12M1/34 ,  C12M1/26 ,  C12M1/02 ,  C12N5/0735 ,  C12N5/074 ,  C12N5/0775 ,  C12N5/10

    摘要： Provided is a cell culture apparatus including a cell supply unit that supplies cells; a culture medium supply unit that supplies a culture medium; an additive supply unit that supplies an additive for inducing the differentiation of undifferentiated cells; a stirring unit that stirs a processing target; a separation unit that separates a component contained in the processing target; a culture vessel that cultures the cells; a first flow channel that forms a circulation route passing through the cell supply unit, the stirring unit, the separation unit, and the culture vessel; a second flow channel that connects the culture medium supply unit and the first flow channel; a third flow channel that connects the additive supply unit and the first flow channel; and a control unit that controls the feeding of liquid through the first flow channel, the second flow channel, and the third flow channel.

    METHOD FOR ENHANCING EFFICACY OF STEM CELL BY USING ETHIONAMIDE
    29.
    发明公开
    METHOD FOR ENHANCING EFFICACY OF STEM CELL BY USING ETHIONAMIDE 审中-公开

    公开(公告)号：EP4001400A2

    公开(公告)日：2022-05-25

    申请号：EP20839615.0

    申请日：2020-07-15

    申请人： Samsung Life Public Welfare Foundation

    发明人： NA, Duk Lyul ,  CHANG, Jong Wook ,  SON, Hyo Jin

    IPC分类号： C12N5/0735 ,  A61K35/545 ,  A61P25/28 ,  A61P29/00

    摘要： The present invention relates to a medium composition for enhancing the efficacy of stem cells, including ethionamide, a method of enhancing the efficacy of stem cells, including culturing stem cells in the medium composition, a method of preparing stem cells with enhanced efficacy, stem cells prepared by the above-mentioned method, and a use thereof. According to the present invention, the anti-inflammatory effect of mesenchymal stem cells and expression levels of paracrine factors may be effectively improved by a simple method of treating mesenchymal stem cells with ethionamide, and the stem cells obtained by the above method may be effectively used for preventing or treating an inflammatory disease or a degenerative brain disease.