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31.发明授权
公开(公告)号：EP0212444B1
公开(公告)日：1992-03-25
申请号：EP86110852.0
申请日：1986-08-06
发明人： Jessee, Joel A. , Bloom, Fredric R.
IPC分类号： C12N15/63
CPC分类号： C12N15/63
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32.发明公开
公开(公告)号：EP0350962A2
公开(公告)日：1990-01-17
申请号：EP89112988.4
申请日：1989-07-14
IPC分类号： G01N27/26
CPC分类号： G01N27/44704
摘要： An apparatus provides for casting a gel and for presenting the gel for electrophoresis. The apparatus comprises a casting tray and the casting plate which combine to form a mold for the gelable liquid, and an electrophoresis tank in which the electrophoresis process occurs. The floor of the electrophoresis tank rises to form the plate pedestal upon which the casting plate rests during electrophoresis. The floor of the electrophoresis tank forms a cavity at the edges of the plate pedestal in which the casting tray rests during the electrophoresis process. The casting plate is first firmly seated in the bottom of the casting tray forming a mold for the gelable liquid. The gelable liquid is poured into the mold. The comb is placed in the comb slots so as to form wells in the gel. The gelable liquid is allowed to gel, and the comb is removed. The material to undergo electrophoresis is placed in the wells left by the comb. The entire assembly is then placed and aligned on the plate pedestal. The casting tray is then pushed down into the casting tray well. Electrophoresis buffer sufficient to fill the tank to approximately the top of the gel is then poured into the electrophoresis tank. An alternate embodiment is shown.
摘要翻译： 一种装置提供了凝胶凝胶并呈现凝胶进行电泳。 该装置包括铸造托盘和组合形成用于可凝胶化液体的模具的流延板和其中发生电泳过程的电泳槽。 电泳槽的地板上升，形成电镀板上放置板的底座。 电泳槽的底部在板基座的边缘形成一个空腔，其中铸造托盘在电泳过程中搁置。 铸造板首先牢固地安置在铸造托盘的底部，形成用于可凝胶化液体的模具。 将可凝胶的液体倒入模具中。 将梳子放置在梳子槽中，以便在凝胶中形成孔。 使凝胶状液体凝胶化并除去梳子。 将进行电泳的材料置于由梳子留下的孔中。 然后将整个组件放置并对准在板基座上。 然后将铸造托盘向下推入铸造盘中。 然后将足以将罐填充至凝胶顶部的电泳缓冲液倒入电泳槽中。 示出了替代实施例。
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公开(公告)号：EP0268651A4
公开(公告)日：1989-07-26
申请号：EP87903775
申请日：1987-05-14
发明人： HARTLEY JAMES L , CAMPBELL JAMES H
摘要： A device (10) for use with culture media for uniformly spreading a liquid suspension thereon comprising a flat, sterile open fabric adapted to lie on the surface (12) of the medium (14), wherein the fabric produces a capillary effect on the liquid to distribute the liquid uniformly across the medium surface (12).
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公开(公告)号：EP0304195A2
公开(公告)日：1989-02-22
申请号：EP88307181.3
申请日：1988-08-03
发明人： Berninger, Mark , Schuette, Michael
IPC分类号： G01N27/26
CPC分类号： G01N27/44704
摘要： An apparatus for casting an agarose gel and for conducting an electrophoresis process is disclosed. An electrophoresis tank (106) supports a gel casting deck (202) having open ends (210) which are sealed by wedge dams (302) to form a cavity for containing the molten agarose as it gels. The wedge dams (302) are pulled downward by gravity into wedge-shaped slots. The weight of the wedge dams (302) in the wedge slots presses the wedge dams (302) against the open ends (210) of the gel casting deck (202) so as to provide a substantially fluid-tight seal. The gel casting deck (202) has sidewalls whcih are slanted toward one another in the upwards direction so that when the gel is submerged in buffer solution during electrophoresis, the gel's tendency to float is substantially impeded. The combs (408) which form wells in the gel are referenced to the sides (208) of the gel casting deck (202) itself so as to ensure uniformity and accuracy of well depth. Safety features prevent the electrophoresis tank from functioning if removed from its protective housing.
摘要翻译： 公开了一种用于浇铸琼脂糖凝胶和进行电泳过程的装置。 电泳槽（106）支撑具有开口端（210）的凝胶浇注甲板（202），所述开口端部（210）由楔形坝（302）密封，以形成用于在凝胶凝固时容纳熔融琼脂糖的空腔。 楔形坝（302）通过重力被向下拉入楔形槽。 楔槽中的楔形坝（302）的重量将楔形坝（302）压靠在凝胶铸造板（202）的开口端（210）上，以便提供基本上不流体密封的密封。 凝胶浇铸板（202）具有沿向上方向彼此倾斜的侧壁，使得当凝胶在电泳期间浸没在缓冲溶液中时，凝胶的浮动倾向被大大阻碍。 在凝胶中形成孔的梳子（408）参考凝胶浇铸板（202）本身的侧面（208），以确保井深的均匀性和精度。 如果从保护壳中取出，安全功能可防止电泳槽起作用。
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35.发明公开
公开(公告)号：EP0212444A3
公开(公告)日：1988-08-24
申请号：EP86110852
申请日：1986-08-06
发明人： Jessee, Joel A. , Bloom, Fredric R.
IPC分类号： C12N15/00
CPC分类号： C12N15/63
摘要： Described is a process for producing transformable, competent cells including the steps of growing the cells in a growth conducive medium at a temperature of less than 37°C and freezing the cells, and cells produced by the process.
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公开(公告)号：EP0175689A4
公开(公告)日：1988-03-03
申请号：EP84901695
申请日：1984-04-06
发明人： HARTLEY JAMES L , BERNINGER MARK S
CPC分类号： C12Q1/706 , C12N15/64 , C12Q1/68 , C12Q1/6876 , Y10S435/803
摘要： A process for detecting specific nucleotide sequences, called targets, in which a special DNA probe molecule, called a probe-vector, is capable of transforming bacteria if and only if it is held in a circular configuration by base pairing to a target nucleic acid, said transformation resulting in the detection of a phenotype specified by the probe-vector, said detection establishing the presence, absence, or quantity of the target; and a probe-vector molecule for performing the process.
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37.发明公开Process of producing highly transformable cells and cells produced thereby 失效一种用于制备hochtransformierbaren细胞，从而转化的细胞的过程。
公开(公告)号：EP0212444A2
公开(公告)日：1987-03-04
申请号：EP86110852.0
申请日：1986-08-06
发明人： Jessee, Joel A. , Bloom, Fredric R.
IPC分类号： C12N15/63
CPC分类号： C12N15/63
摘要： Described is a process for producing transformable, competent cells including the steps of growing the cells in a growth conducive medium at a temperature of less than 37°C and freezing the cells, and cells produced by the process.
摘要翻译： 描述了一种用于制造可变形，感受态细胞包括由过程产生的在低于37℃的温度下在生长培养基利于生长细胞并冷冻该细胞的步骤，和细胞的方法。
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公开(公告)号：EP2884906B1
公开(公告)日：2020-05-06
申请号：EP13829525.8
申请日：2013-08-15
发明人： MEDEMA, Ryan , NELSON, Jeffrey , LIOI, Anthony , BAKER, Clyde , SOUTHARD, Fletcher D. , SEELEY, Mark E.
IPC分类号： A61B17/04 , A61F2/24 , A61L17/10 , A61B17/06 , A61B17/00 , A61B17/064 , A61L17/04 , A61L17/12 , A61B17/062
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公开(公告)号：EP2714236A1
公开(公告)日：2014-04-09
申请号：EP12789056.4
申请日：2012-05-18
IPC分类号： B01D39/00
CPC分类号： B01J20/3416 , B01D15/203 , B01D39/2055 , B01D53/02 , B01D53/965 , B01D2253/102 , B01D2253/25 , B01J20/046 , B01J20/20 , B01J20/3204 , B01J20/3236 , B01J20/3441 , C02F1/283 , C02F1/288 , C02F1/766 , C02F2303/04 , C02F2303/16 , C02F2305/04
摘要： Contaminants are filtered from a fluid flow stream and the filter is regenerated by a process including steps of: providing a filter material comprising both carbon and potassium iodide; passing a contaminated fluid stream in contact with the filter material; adsorbing contaminants from the fluid stream onto surfaces in the filter material; passing an electric current through the filter material with adsorbed contaminant thereon; disassociating contaminant from the surfaces of the filter material; and removing disassociated contaminant from the filter material by carrying away the disassociated contaminant in a fluid flow mass. Separately, a stable, active iodine solution is also provided for numerous deodorizing and disinfecting applications.
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公开(公告)号：EP1842495A3
公开(公告)日：2010-07-07
申请号：EP07105647.7
申请日：2007-04-04
IPC分类号： A61B17/11
CPC分类号： A61B17/11 , A61B17/0643 , A61B17/115 , A61B2017/0641 , A61B2017/1103 , A61B2017/1107
摘要： The invention includes an assembly for anastomosis having a clamp being arranged to support a fastener. The clamp can be actuated to join a vessel with the fastener to establish a fluid communication. In some embodiments, the invention includes an assembly having a clamp and a fastener with first and second joinable parts, and the assembly can be adapted to facilitate the alignment of the first and second joinable parts.
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