ANALYSIS OF DNA
    41.
    发明公开
    ANALYSIS OF DNA 审中-公开
    DNA - 分析

    公开(公告)号:EP2992083A1

    公开(公告)日:2016-03-09

    申请号:EP14791467.5

    申请日:2014-05-01

    IPC分类号: C12M1/34

    摘要: The invention provides pyrosequencing-based methods of analyzing and synthesizing DNA, including methods of DNA error correction, determining DNA size distribution, screening for nucleotide repeat disorders such as fragile X syndrome, determining size distribution and bias in a DNA library, and determining pyrosequencing read length. The methods include on-bench protocols as well as droplet-based protocols that may be conducted on a droplet actuator.

    摘要翻译: 本发明提供了基于焦磷酸测序的分析和合成DNA的方法,包括DNA错误校正的方法,确定DNA大小分布,筛选核苷酸重复病症如脆性X综合征,确定DNA文库中的大小分布和偏差,以及确定焦磷酸测序读数 长度。 这些方法包括工作台协议以及可以在液滴致动器上进行的基于液滴的协议。

    Microfluidic Feedback Using Impedance Detection
    42.
    发明公开
    Microfluidic Feedback Using Impedance Detection 审中-公开
    MikrofluidischeRückkoppelung手套Impedanzmessung

    公开(公告)号:EP2711079A2

    公开(公告)日:2014-03-26

    申请号:EP13192679.2

    申请日:2012-05-08

    IPC分类号: B01L3/00 G01N27/02 G01N33/18

    摘要: A method, comprising: generating an output voltage by a power supply; storing charge produced by the output voltage; superimposing an excitation signal onto the output voltage to produce a superimposed signal; connecting the superimposed signal to an electrode in a droplet actuator; suppressing the output voltage from the power supply when detecting an impedance at the electrode; supplying the charge to the droplet actuator to activate the electrode during the impedance; and measuring the impedance produced by the excitation signal while the output voltage is suppressed, wherein the impedance indicates presence of liquid at the electrode. Superimposing the excitation signal may comprise adding the excitation signal to the output voltage. Suppressing the output voltage may comprise at least one of stopping the output voltage generated by the power supply, disabling a switching action of the power supply, and disabling the power supply during the impedance measurement.

    摘要翻译: 一种方法,包括:通过电源产生输出电压; 存储由输出电压产生的电荷; 将激励信号叠加到输出电压上以产生叠加信号; 将叠加的信号连接到液滴致动器中的电极; 当检测到电极处的阻抗时,抑制来自电源的输出电压; 将电荷供应到液滴致动器以在阻抗期间激活电极; 并且在输出电压被抑制的同时测量由激励信号产生的阻抗,其中阻抗表示在电极处存在液体。 激励信号的叠加可以包括将激励信号加到输出电压上。 抑制输出电压可以包括停止由电源产生的输出电压,禁止电源的开关动作和在阻抗测量期间禁用电源中的至少一个。

    DROPLET ACTUATOR ASSEMBLIES AND METHODS OF MAKING SAME
    43.
    发明公开
    DROPLET ACTUATOR ASSEMBLIES AND METHODS OF MAKING SAME 有权
    TROPFEN-STELLGLIEDBAUGRUPPEN UND VERFAHREN ZUR HERSTELLUNG DAVON

    公开(公告)号:EP2588322A4

    公开(公告)日:2013-12-25

    申请号:EP11810072

    申请日:2011-06-24

    IPC分类号: B41J2/175 B41F31/08

    摘要: The invention provides droplet actuator assemblies and systems and methods of manufacturing the droplet actuator assemblies. In certain embodiments, two-piece enclosures are used to form a droplet actuator assembly that houses a droplet operations substrate. In certain other embodiments, one-piece enclosures are used to form a droplet actuator assembly that houses a droplet operations substrate. In the plastic injection molding process for forming substrates of the droplet actuator assemblies of the present invention may utilize insert molding (or overmolding) processes for forming a gasket in at least one substrate, thereby avoiding the need for providing and installing a separate gasket component. Further, the droplet actuator assemblies may include features that allow ultrasonic welding processes to be used for bonding substrates together. The manufacturing systems of the present invention for fabricating the droplet actuator assemblies may utilize continuous flow reel-to-reel manufacturing processes.

    摘要翻译: 本发明提供了液滴致动器组件以及制造液滴致动器组件的系统和方法。 在某些实施例中,两件式外壳用于形成容纳液滴操作基板的液滴致动器组件。 在某些其他实施例中,使用单件式外壳来形成容纳液滴操作基板的液滴致动器组件。 在用于形成本发明的液滴致动器组件的基底的塑料注射成型工艺中,可以利用用于在至少一个基底中形成垫圈的嵌入成型(或包覆成型)工艺,从而避免提供和安装单独的垫圈部件的需要。 此外,液滴致动器组件可以包括允许使用超声波焊接工艺将基板粘合在一起的特征。 用于制造液滴致动器组件的本发明的制造系统可以利用连续流量的卷对卷制造工艺。