公开(公告)号：EP2734290A4

公开(公告)日：2015-03-25

申请号：EP12814718

申请日：2012-07-23

申请人： EMD MILLIPORE CORP

发明人： KAS ONUR Y ,  KOZLOV MIKHAIL ,  TKACIK GABRIEL ,  NHIEM DAVID ,  GODDARD PHILIP ,  LEON SHERRY ASHBY

IPC分类号： B01D69/12 ,  B01D35/00 ,  B01D39/08 ,  B01D39/16 ,  B01D61/14 ,  B01D69/02 ,  C02F1/44

CPC分类号： B01D69/02 ,  B01D67/0004 ,  B01D2239/025 ,  B01D2239/0618 ,  B01D2239/0631 ,  B01D2239/0654 ,  B01D2323/39 ,  C02F1/444 ,  C02F2303/04

公开(公告)号：EP2788106A4

公开(公告)日：2015-03-11

申请号：EP12856081

申请日：2012-12-10

申请人： EMD MILLIPORE CORP

发明人： NGAN CLIFTON ,  ZOU YU ,  BARTLETT ANDREW ,  HILLIER BRIAN

IPC分类号： B01D63/08 ,  B01D39/08 ,  B01D69/00 ,  C07K1/34 ,  C12M1/12

CPC分类号： B01D35/28 ,  B01D39/083 ,  B01D63/08 ,  B01D2313/10 ,  B01D2313/143 ,  C07K1/34

公开(公告)号：EP2707708A4

公开(公告)日：2014-12-24

申请号：EP12781832

申请日：2012-05-10

申请人： EMD MILLIPORE CORP

发明人： SCOTT CHRIS ,  CLARK PHILLIP ,  GREENIZEN KURT E ,  AMARA RYAN A ,  COLONNA ROBERT

IPC分类号： G01N31/22 ,  B01L3/00 ,  G01N33/543

CPC分类号： G01N33/558 ,  B01J20/28033 ,  B01J20/28035 ,  B01J20/2804 ,  B01L3/5023 ,  B01L9/52 ,  B01L2200/025 ,  B01L2300/0609 ,  B01L2300/0681 ,  B01L2300/069 ,  B01L2300/0809 ,  B01L2300/12 ,  B01L2300/126 ,  B01L2400/0487 ,  B01L2400/049 ,  G01N33/54366 ,  G01N33/56911 ,  G01N2469/10 ,  G01N2469/20

摘要： The embodiments disclosed herein are directed to an apparatus useful in conducting detection of compounds on blotting membranes. The device is comprised of several layers including a porous support layer below the blotting membrane(s), a flow distributor above the blotting membrane(s) and optionally a well on the flow distributor to contain the liquid to the desired area and to allow for lower starting volumes of such liquid. Preferably, the flow distributor is a non-binding or low binding hydrophilic porous membrane such as a 0.22 micron membrane and the support layer is a grid or sintered porous material. The distributor and support are held together to form an envelope around the membrane(s). The use of a hinge, clips and other such devices is preferred in doing so.

公开(公告)号：EP2691182A4

公开(公告)日：2014-09-17

申请号：EP12764963

申请日：2012-03-28

申请人： EMD MILLIPORE CORP ,  JOUETTE SEBASTIAN

发明人： JOUETTE SEBASTIAN ,  FELDEN LUC

IPC分类号： B04B7/16 ,  B01D17/038 ,  B01D61/00 ,  B04B1/00

CPC分类号： B01D21/262 ,  B01D17/0217 ,  B01D17/085 ,  B01D61/18 ,  B01D63/16 ,  B01D71/34 ,  B01D2315/02 ,  B04B5/04 ,  B04B5/0407 ,  B04B2005/0435 ,  B04B2005/0478 ,  B04B2005/0485

摘要： The invention provides a filtering and centrifugation device, comprising a barrel, a volume in the barrel for receiving a fluid sample to be processed, a filter medium, at least one piston movable in the barrel to force fluid in the volume through the filter medium to produce a filtrate, and a space for pelleting particulate material. The device is centrifugeable, and said space is located in or communicates with the volume and is located preferably away from the filter medium such that the particulate material does not or at least not completely clog the filter medium during such centrifugation of the device. The invention also provides a method for the sterile filtration of a sample, comprising the steps of introducing the sample into the volume of such a device, subjecting the device to centrifugation to pellet particulate material contained in the sample at the space away from the filter medium, and applying force to the piston(s) to force fluid of the sample in the volume through the filter medium to produce a filtrate. By providing the space for pelleting the particulate material under the influence of the centrifugation force, the clogging of the filter medium can be avoided or considerably delayed. Further, the entire process can be performed in the same device without having to remove the sample for any intermediate clarification steps.

公开(公告)号：EP2370561A4

公开(公告)日：2013-07-24

申请号：EP09835506

申请日：2009-12-08

申请人： EMD MILLIPORE CORP

发明人： MOYA WILSON ,  DUPONT ALISON

IPC分类号： C12M1/00

CPC分类号： C12M27/00 ,  C12M23/20 ,  C12M23/28 ,  C12M47/12

摘要： Container for sample preparation or processing, such as biomass culturing or processing, and optionally sample purification. In certain embodiments, the reactor is a bioreactor that includes a stirred cell device that simulates a tangential flow filter to reduce or eliminate clogging that can be caused by the solids generated. In certain embodiments, the solids comprise a precipitate or floc or beads, such as one that includes a polymer that binds the biomolecule(s) of interest, and impurities. In its method aspects, embodiments disclosed herein include purification and isolation of biomolecules of interest derived from cell culture fluids. The methods include carrying out sample preparation or processing in a container, culturing a biomass; generating solids by precipitating or flocculating a biomolecule of interest from the cultured broth; preventing the solids from settling in the container by agitation; and purification, such as by eluting the biomolecule of interest and filtering the same.

公开(公告)号：EP1185616A4

公开(公告)日：2003-04-23

申请号：EP00930821

申请日：2000-05-19

申请人： MILLIPORE CORP

发明人： PRESENTE ESTHER ,  YOUNG BARBARA ,  UPPERMAN SUSAN

IPC分类号： G01N33/483 ,  C12Q1/02 ,  C12Q1/04 ,  C12Q1/22 ,  C12Q1/42 ,  C12Q1/48 ,  C12Q1/66 ,  G01N21/78 ,  C12N1/06 ,  C12N13/00 ,  C12Q1/06 ,  C12Q1/34

CPC分类号： C12Q1/48 ,  C12Q1/04 ,  C12Q1/22 ,  C12Q1/66 ,  C12Q2304/60

摘要： A method for rapidly detecting and enumerating microorganisms, having a level of microbial ATP, in the presence of mammalian cell preparations, comprising the steps of: providing a mammalian cell preparation comprising mammalian cells having a level of mammalian ATP; reducing the level of mammalian ATP in the cell preparation by, differentially lyzing the mammalian cells with detergent or osmotic shock to extract the mammalian ATP, treating the extracted mammalian ATP with one or more ATP hydrolyzing compounds; and immobilizing the microorganisms and washing away the detergent and the hydrolyzing enzyme by filtering the mammalian cell preparation through a micropartitioned hydrophilic/hydrophobic membrane; extracting the microbial ATP using an extracting reagent; drying the membrane; applying a bioluminescent reagent onto the membrane; and detecting and enumerating the microorganisms.

公开(公告)号：EP0803474A3

公开(公告)日：1998-07-08

申请号：EP97300606

申请日：1997-01-30

申请人： MILLIPORE CORP

发明人： DENONCOURT JEFFREY P ,  MOULIN JACQUES

IPC分类号： B01D61/42 ,  B01D61/48 ,  B01D61/52 ,  B01J47/08 ,  C02F1/42 ,  C02F1/46 ,  C02F1/469

CPC分类号： C02F1/4695 ,  B01D61/48 ,  B01D61/52 ,  B01J47/08 ,  C02F1/42 ,  C02F1/4604 ,  C02F2201/46115

摘要： An electrodeionization apparatus has a cathode compartment (32) and an anode compartment (34) for removing ions from a liquid (26) under the influence of an applied voltage, and the cathode compartment (32) includes electrically conductive particles such as metal particles and/or carbon particles.

公开(公告)号：EP3062919A4

公开(公告)日：2017-12-20

申请号：EP14856842

申请日：2014-10-03

申请人： EMD MILLIPORE CORP

发明人： WOOD AMY ,  KRAUS DAVE ,  HANSEN ANNE ,  DER KARA ,  MCSWEENEY JAMES

IPC分类号： B01F3/04 ,  B01F15/00

CPC分类号： C12M27/02 ,  B01F3/04269 ,  B01F3/04531 ,  B01F7/22 ,  B01F13/0863 ,  B01F15/0022 ,  B01F15/00344 ,  B01F15/0085 ,  B01F2003/04297 ,  B01F2003/04631 ,  B01F2003/04673 ,  B01F2215/0073 ,  C12M29/06 ,  C12Q3/00

摘要： An aeration device, and containers or vessels incorporating the same. The aeration device can comprise a plurality of interchangeable aeration elements that can produce gas bubble of different sizes and deliver them to the contents of the container. Also disclosed are containers, such as a disposable or single-use container, optionally having one or more inlets and one or more outlets, an aeration device including a plurality of aeration elements, and a mixer to cause mixing, dispersing, homogenizing and/or circulation of one or more ingredients contained or added to the container. The container can be a bioreactors and the aeration device controls the dissolved gas concentration content of the bioreactor contents, thereby facilitating proper growth of cell cultures in the bioreactor.

公开(公告)号：EP2598223A4

公开(公告)日：2017-07-19

申请号：EP11813102

申请日：2011-07-27

申请人： EMD MILLIPORE CORP

发明人： YAVORSKY DAVID ,  AMARA JOHN ,  UMANA JOAQUIN ,  CATALDO WILLIAM ,  KOZLOV MIKHAIL ,  STONE MATTHEW

IPC分类号： B01D15/36 ,  B01J20/28 ,  B01J20/285 ,  C07K1/18

CPC分类号： B01J39/26 ,  B01D15/327 ,  B01D15/361 ,  B01D15/362 ,  B01D15/363 ,  B01D15/3804 ,  B01J20/28023 ,  B01J20/28059 ,  B01J20/285 ,  B01J20/287 ,  B01J20/3244 ,  B01J41/20 ,  B01J47/014 ,  B01J47/127 ,  C07K1/18 ,  C07K16/00 ,  C12N7/00 ,  C12N7/02 ,  Y10S502/52724 ,  Y10T428/2938 ,  Y10T428/2973

摘要： Adsorptive media for chromatography, particularly ion-exchange chromatography, derived from a shaped fiber. In certain embodiments, the functionalized shaped fiber presents a fibrillated or ridged structure which greatly increases the surface area of the fibers when compared to ordinary fibers. Also disclosed herein is a method to add surface pendant functional groups that provides cation-exchange or anion-exchange functionality to the high surface area fibers. This pendant functionality is useful for the ion-exchange chromatographic purification of biomolecules, such as monoclonal antibodies (mAbs).

摘要翻译： 用于色谱的吸附介质，特别是离子交换色谱，由成形纤维衍生而来。 在某些实施方案中，官能化成形纤维呈现原纤化或脊状结构，与普通纤维相比，其大大增加了纤维的表面积。 本文还公开了向高表面积纤维提供阳离子交换或阴离子交换官能团的表面侧挂官能团的方法。 该侧链功能可用于生物分子（如单克隆抗体（mAbs））的离子交换色谱纯化。

公开(公告)号：EP2656061A4

公开(公告)日：2017-04-05

申请号：EP11855025

申请日：2011-12-07

申请人： EMD MILLIPORE CORP

发明人： SCOTT CHRIS ,  COLONNA ROB ,  BARMORE CHRISTOPHER ,  TAYLOR TODD ,  MORGAN DANIEL ,  DESILETS KENNETH G

IPC分类号： G01N30/14 ,  B01L3/00

CPC分类号： G01N1/28 ,  B01D61/18 ,  B01J20/281 ,  B01L3/50255 ,  G01N1/4077 ,  G01N30/14 ,  G01N2030/146

摘要： Sample preparation device for receiving in sealing relation one or more filter assemblies and one or more sample containers, each container uniquely positioned within the device to receive filtered sample from a designated filter. The device includes a body, a lid disposed on the body, a container tray, a base, an integrated seal on the body, a valve and a valve actuator. The tray can be disposed in a vacuum chamber defined by the lid and the body, and one or more sample containers can be disposed in the tray. When properly positioned in the tray in the vacuum chamber, each container is in fluid communication with a single respective filter assembly disposed in sealing relation in a respective aperture in the lid. A single actuation of a valve causes the lid to seal to the base and drives the simultaneous filtration of a plurality of samples.

摘要翻译： 用于以密封关系接收一个或多个过滤器组件和一个或多个样品容器的样品制备装置，每个容器唯一地定位在装置内以从指定的过滤器接收经过过的样品。 该装置包括主体，设置在主体上的盖，容器托盘，基座，主体上的集成密封件，阀和阀致动器。 托盘可以设置在由盖和主体限定的真空室中，并且一个或多个样品容器可以设置在托盘中。 当适当地定位在真空室中的托盘中时，每个容器与在盖中的相应孔中以密封关系设置的单个相应的过滤器组件流体连通。 阀的单个致动导致盖子密封到基座并驱动多个样品的同时过滤。