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公开(公告)号：EP2536854B1
公开(公告)日：2017-07-19
申请号：EP11745196.3
申请日：2011-02-17
CPC分类号： C12Q1/6886 , C12Q2600/156
摘要： Clinical management of human cancer is dependent on the accurate monitoring of residual and recurrent tumors. We have developed a method, called personalized analysis of rearranged ends (PARE), which can identify translocations in solid tumors. Analysis of four colorectal and two breast cancers revealed an average of nine rearranged sequences (range 4 to 15) per tumor. Polymerase chain reaction with primers spanning the breakpoints were able to detect mutant DNA molecules present at levels lower than 0.001% and readily identified mutated circulating DNA in patient plasma samples. This approach provides an exquisitely sensitive and broadly applicable approach for the development of personalized biomarkers to enhance the clinical management of cancer patients.
摘要翻译： 人类癌症的临床管理依赖于准确监测残留和复发性肿瘤。 我们开发了一种称为重排末端的个性化分析（PARE）的方法，可以识别实体瘤中的易位。 对四个结直肠癌和两个乳腺癌的分析显示每个肿瘤平均有9个重排序列（范围4-15）。 使用跨越断点的引物进行的聚合酶链式反应能够检测到存在的水平低于0.001％的突变DNA分子，并且易于鉴定患者血浆样品中的突变循环DNA。 这种方法为开发个性化生物标志物以提高癌症患者的临床管理提供了一种非常灵敏和广泛适用的方法。
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公开(公告)号：EP2988760A1
公开(公告)日：2016-03-02
申请号：EP14774988.1
申请日：2014-03-28
IPC分类号： A61K35/66
CPC分类号： A61K35/74 , A61K31/65 , A61K35/742 , A61K45/06 , A61K2035/11 , A61N5/10
摘要： The present invention provides, inter alia, methods for treating or ameliorating an effect of a solid tumor present in a human. These methods include administering intratumorally to the human a unit dose of C. novyi, preferably C. novyi NT, colony forming units (CFUs), which contains about 1 x 10
3 -1 x 10
7 CFUs suspended in a pharmaceutically acceptable carrier or solution. Methods for debulking a solid tumor present in a human, methods for ablating a solid tumor present in a human, a method for microscopically precise excision of tumor cells in a human, methods for treating or ameliorating an effect of a solid tumor that has metastasized to one or more sites in a human, unit doses of C. novyi, preferably C. novyi NT, CFUs, and kits for treating or ameliorating an effect of a solid tumor present in a human are also provided.-
公开(公告)号：EP1730303B1
公开(公告)日：2013-07-31
申请号：EP05723277.9
申请日：2005-02-18
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q2600/156
摘要： Phosphatidylinositol 3-kinases (PI3Ks) are known to be important regulators of signaling pathways. To determine whether PI3Ks are genetically altered in cancers, we analyzed the sequences of the P13K gene family and discovered that one family member, PIK3CA, is frequently mutated in cancers of the colon and other organs. The majority of mutations clustered near two positions within the P13K helical or kinase domains. PIK3CA represents one of the most highly mutated oncogenes yet identified in human cancers and is useful as a diagnostic and therapeutic target.
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公开(公告)号：EP2417270A2
公开(公告)日：2012-02-15
申请号：EP10762298.7
申请日：2010-04-06
发明人： VOGELSTEIN, Bert , KINZLER, Kenneth, W. , LI, Meng , DIAZ, Luis , PAPADOPOULOS, Nickolas , MARKOWITZ, Sanford
CPC分类号： C12Q1/6816 , C12Q1/6886 , C12Q2600/154 , C12Q2565/501 , C12Q2527/125 , C12Q2523/125
摘要： Abnormal DNA methylation can be used as a biomarker in cancer patients. For such purposes, it is important to determine precisely the fraction of methylated molecules in an analyzed sample. A technology we term Methyl-BEAMing achieves this goal. Individual bisulfite-treated DNA molecules can be PCR-amplified within aqueous nanocompartments containing beads, resulting in a population of beads each containing thousands of copies of the template molecule. After hybridization with probes specific for methylated sequences, the beads can be analyzed by flow cytometry. This approach enables detection and enumeration of one methylated molecule in a population of ~5000 unmethylated molecules. Methyl-BEAMing provides digital quantification of rare methylation events and is generally applicable to the assessment of methylated genes in clinical samples.
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公开(公告)号：EP1056881B1
公开(公告)日：2007-05-23
申请号：EP99908441.1
申请日：1999-02-25
CPC分类号： C12N15/86 , C07K14/005 , C12N2710/10322 , C12N2710/10343 , C12N2830/38
摘要： Recombinant adenoviruses provide a versatile system for gene expression studies and therapeutic applications. This invention describes a strategy which simplifies the generation and production of such viruses. A recombinant adenoviral plasmid is generated with a minimum of enzymatic manipulations, employing homologous recombination in bacteria rather than in eucaryotic cells. Following transfections of such plasmids into a mammalian packaging cell line, viral production can be conveniently followed with the aid of green fluorescent protein, encoded by a gene incorporated into the viral backbone. Homogeneous viruses can be obtained from this procedure without plaque purification. This system expedites the process of generating and testing recombinant adenoviruses.
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46.发明公开
公开(公告)号：EP1641809A2
公开(公告)日：2006-04-05
申请号：EP04752581.1
申请日：2004-06-09
CPC分类号： C12Q1/6858 , C07H21/04 , C12N15/1075 , C12Q1/686 , C12Q2565/537 , C12Q2563/149 , C12Q2563/143
摘要： Many areas of biomedical research depend on the analysis of uncommon variations in individual genes or transcripts. Here we describe a method that can quantify such variation at a scale and ease heretofore unattainable. Each DNA molecule in a collection of such molecules is converted into a single particle to which thousands of copies of DNA identical in sequence to the original are bound. This population of beads then corresponds to a one-to-one representation of the starting DNA molecules. Variation within the original population of DNA molecules can then be simply assessed by counting fluorescently-labeled particles via flow cytometry. Millions of individual DNA molecules can be assessed in this fashion with standard laboratory equipment. Moreover, specific variants can be isolated by flow sorting and employed for further experimentation. This approach can be used for the identification and quantification of rare mutations as well as to study variations in gene sequences or transcripts in specific populations or tissues.
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47.发明授权HUMAN MUTATOR GENE hMSH2 AND HEREDITARY NON POLYPOSIS COLORECTAL CANCER 失效人增变-GEN NMSH2及其连接TO HEREDITAREN，非息肉性KOLORREKTALEN癌症
公开(公告)号：EP0730648B1
公开(公告)日：2004-08-11
申请号：EP95904201.1
申请日：1994-12-02
CPC分类号： C07K14/82 , A01K2217/05 , A61K38/00 , A61K48/00 , C12Q1/6886 , C12Q2600/106 , C12Q2600/136 , C12Q2600/156
摘要： The human MSH2 gene, responsible for hereditary non-polyposis colorectal cancer, was identified by virtue of its homology to the MutS class of genes, which are involved in DNA mismatch repair. The sequence of cDNA clones of the human gene are provided, and the sequence of the gene can be used to demonstrate the existence of germ line mutations in hereditary non-polyposis colorectal cancer (HNPCC) kindreds, as well as in replication error (RER ) tumor cells.
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48.发明授权AN APC MUTATION ASSOCIATED WITH FAMILIAL COLORECTAL CANCER IN ASHKENAZI JEWS 失效在德系犹太人相关的家族性结肠癌甲APC突变GOES
公开(公告)号：EP0981644B1
公开(公告)日：2003-05-14
申请号：EP98903558.9
申请日：1998-01-21
IPC分类号： C12Q1/68 , G01N33/574
CPC分类号： G01N33/574 , C12Q1/6886 , C12Q2600/156 , G01N33/57419 , Y10S435/81 , Y10S436/813
摘要： During routine screening of a patient with a family history of colorectal cancer for truncating APC mutations, a novel missence mutation was identified. Upon further evaluation, it was found that 6 % of Ashkenazi Jews carry this mutation, and that it was present in ∩20 % of Ashkenazis with a family history of CRC. Probes, methods, and kits for identifying individuals affected with this missense mutation are provided.
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49.发明授权
公开(公告)号：EP0569527B1
公开(公告)日：2001-03-14
申请号：EP92906080.4
申请日：1992-01-16
申请人： THE JOHNS HOPKINS UNIVERSITY , ZENECA LIMITED , THE UNIVERSITY OF UTAH , JAPANESE FOUNDATION FOR CANCER RESEARCH
发明人： KINZLER, Kenneth, W. , VOGELSTEIN, Bert , ANAND, Rakesh , HEDGE, Philip, John , MARKHAM, Alexander, Fred , ALBERTSEN, Hans , CARLSON, Mary, L. , GRODEN, Joanna, L. , JOSLYN, Geoff , THLIVERIS, Andrew , WHITE, Raymond, L. , NAKAMURA, Yusuke
CPC分类号： C12Q1/6827 , C07K14/47 , C12Q1/68 , C12Q1/6886 , C12Q2600/118 , C12Q2600/136
摘要： A human gene termed APC is disclosed. Methods and kits are provided for assessing mutations of the APC gene in human tissues and body samples. APC mutations are found in familial adenomatous polyposis patients as well as in sporadic colorectal cancer patients. APC is expressed in most normal tissues. These results suggest that APC is a tumor suppressor.
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公开(公告)号：EP1056881A1
公开(公告)日：2000-12-06
申请号：EP99908441.1
申请日：1999-02-25
CPC分类号： C12N15/86 , C07K14/005 , C12N2710/10322 , C12N2710/10343 , C12N2830/38
摘要： Recombinant adenoviruses provide a versatile system for gene expression studies and therapeutic applications. This invention describes a strategy which simplifies the generation and production of such viruses. A recombinant adenoviral plasmid is generated with a minimum of enzymatic manipulations, employing homologous recombination in bacteria rather than in eucaryotic cells. Following transfections of such plasmids into a mammalian packaging cell line, viral production can be conveniently followed with the aid of green fluorescent protein, encoded by a gene incorporated into the viral backbone. Homogeneous viruses can be obtained from this procedure without plaque purification. This system expedites the process of generating and testing recombinant adenoviruses.
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