公开(公告)号：EP1664094B1

公开(公告)日：2008-11-12

申请号：EP03774298.8

申请日：2003-11-24

Applicant: Korea Advanced Institute of Science and Technology

Inventor： LEE, Sang Yup ,  HAN, Mee Jung ,  PARK, Si Jae

IPC: C07K14/00

CPC classification number: C07K14/195 ,  C07K14/00

公开(公告)号：EP1924703A1

公开(公告)日：2008-05-28

申请号：EP05787088.3

申请日：2005-09-15

Applicant: Korea Advanced Institute of Science and Technology

Inventor： LEE, Sang Yup ,  WOO, Han Min ,  CHOI, Hyung Seok

IPC: C12Q1/68

CPC classification number: G06F19/12

Abstract: The present invention relates to a method for improving useful substance- producing organisms using metabolic flux analysis, and more particularly to a method for improving a host organism producing a useful substance, the method comprising: calculating a maximum flux value corresponding to the theoretical maximum yield of the useful substance in the metabolic network model of the host organism for producing useful substance, and calculating the optimum value of metabolic flux associated with useful substance production in the metabolic network when the value of cell growth-associated metabolic flux is the maximum under the condition where fermentation data are applied or not applied; selecting metabolic fluxes whose absolute values increase from the range between the maximum value and the optimum value; screening genes associated with the selected metabolic fluxes; and introducing and/or amplifying the selected genes in the host organism. According to the invention, the production of the useful substance can be effectively improved by selecting metabolic fluxes to be amplified and genes involved in the metabolic fluxes from the range between the optimum value and maximum value of production-associated metabolic flux in the host organism for producing the useful substance, whose genome-level metabolic network model is constructed, and introducing and/or amplifying the selected genes in the organism.

公开(公告)号：EP4339292A1

公开(公告)日：2024-03-20

申请号：EP22807762.4

申请日：2022-05-10

Applicant: Korea Advanced Institute of Science and Technology

Inventor： LEE, Sang Yup ,  CHOI, Kyeong Rok ,  YU, Hyeeun

IPC: C12P9/00 ,  C12P17/18 ,  C12N1/20 ,  A23L13/40 ,  A23J3/22 ,  A23L27/26

Abstract: The present invention relates to a food having improved flavor, nutrition and color and a method for producing the same, and more specifically, to a food having a flavor, nutrition and color similar to those of meat, the food containing either a compound selected from the group consisting of heme, porphyrins, metalloporphyrins, and porphyrin analogs, or a microorganism that produces the compound, and a method for producing the same. According to the present invention, it is possible to improve the flavor, nutrition, and color of food by producing heme and various porphyrins, metalloporphyrins, and porphyrin analogs with high efficiency by fermentation of a microbial mutant, isolating/purifying the products, and adding the isolated/purified products to food.

公开(公告)号：EP3822354A1

公开(公告)日：2021-05-19

申请号：EP19808016.0

申请日：2019-04-24

Applicant: Korea Advanced Institute Of Science And Technology ,  Hanwha Solutions Corporation

Inventor： LEE, Sang Yup ,  CHO, Jae Sung ,  KIM, Je Woong ,  PRABOWO, Cindy Pricilia Surya ,  HAN, Taehee ,  KO, Yoo Sung

IPC: C12N15/77 ,  C12N15/67 ,  C12N9/04 ,  C12N9/02 ,  C12N9/12 ,  C12P7/18 ,  C07K14/34

Abstract: The present invention relates to recombinant Corynebacterium having 1,3-PDO productivity and reduced 3-HP productivity, and a method for producing 1,3-PDO by using same. When a Corynebacterium glutamicum variant according to the present invention is used, the productivity of 3-HP, which is a by-product, is inhibited by using low-cost glycerol as a carbon source, and thus 1,3-PDO can be produced with high efficiency.

公开(公告)号：EP3608417A1

公开(公告)日：2020-02-12

申请号：EP18899006.3

申请日：2018-08-30

Applicant: Korea Advanced Institute of Science and Technology

Inventor： LEE, Sang Yup ,  YANG, Dongsoo

IPC: C12Q1/48 ,  C12N9/10 ,  C12N15/63 ,  C12P7/22 ,  C12P7/42 ,  C12P17/06

Abstract: The present invention relates to a recombinant microorganism for malonyl-CoA detection in which a type III polyketide synthase-encoding gene is inserted in the genome or in which a recombinant vector containing the gene is introduced; a method of screening a malonyl-CoA production-inducing substance using the recombinant microorganism; a method of screening a gene which is involved in increased malonyl-CoA production; and a method comprising knocking down the gene, screened by the method, in a microorganism, thus increasing the production of malonyl-CoA in the microorganism, and producing a useful substance in the microorganism using malonyl-CoA as a precursor. The use of the biosensor according to the present invention provides single-step signal generation, utilization in various microorganisms, utilization in self-fluorescent microorganisms, a simple construction method, and a simple screening method. In addition, when the present invention is combined with high-throughput screening, it has advantages in that strains having increased malonyl-CoA producing ability can be screened very easily and rapidly (∼3 days) and can be applied directly to the malonyl-CoA-based production of useful compounds.

公开(公告)号：EP3567109A1

公开(公告)日：2019-11-13

申请号：EP18889100.6

申请日：2018-12-12

Applicant: Korea Advanced Institute Of Science And Technology

Inventor： LEE, Sang Yup ,  ZHAO, Xin Rui ,  CHOI, Kyeong Rok

IPC: C12N15/67 ,  C12P17/18

Abstract: The present invention relates to a microorganism variant having the ability to extracellularly produce heme, and more particularly to a metabolically engineered microorganism variant having the ability to extracellularly produce heme and a method of producing heme using the same. According to the present invention, heme, an organometallic compound which is increasingly used as a health food or food supplement for the treatment of porphyria, can be extracellularly secreted and produced in high yield using the microorganism variant, but not conventional chemical synthesis or enzymatic synthesis.

公开(公告)号：EP3409781A1

公开(公告)日：2018-12-05

申请号：EP16888318.9

申请日：2016-12-13

Applicant: Korea Advanced Institute of Science and Technology

Inventor： LEE, Sang Yup ,  CHOI, So Young ,  PARK, Si Jae

IPC: C12N15/63 ,  C12N15/52 ,  C12N9/04 ,  C12N9/10 ,  C12N9/18 ,  C12P7/40

CPC classification number: C12N9/10 ,  C12N9/18 ,  C12N15/52 ,  C12N15/63 ,  C12P7/40

Abstract: The present invention relates to a recombinant microorganism having the ability to produce a poly(lactate-co-glycolate) and a copolymer thereof from xylose and, more specifically, to a recombinant microorganism having the ability to produce poly(lactate-co-glycolate) and a copolymer thereof without the external supply of glycolate precursors, and a method for preparing a poly(lactate-co-glycolate) copolymer by using the same.

公开(公告)号：EP2054502B2

公开(公告)日：2017-06-28

申请号：EP07793240.8

申请日：2007-07-25

Applicant: Korea Advanced Institute of Science and Technology

Inventor： LEE, Sang Yup ,  LIM, Sung Won ,  SONG, Hyohak

IPC: C12N1/21

CPC classification number: C12P7/46 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/1217 ,  C12N15/902 ,  C12Y203/01054 ,  Y02P20/52

公开(公告)号：EP2201115B2

公开(公告)日：2016-07-20

申请号：EP08793214.1

申请日：2008-08-13

Applicant: LG Chem, Ltd. ,  Korea Advanced Institute of Science and Technology

Inventor： PARK, Si Jae ,  LEE, Sang Hyun ,  LEE, Sang Yup ,  LEE, Eun Jeong

IPC: C12N15/10

CPC classification number: C12N15/77 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/13 ,  C12P7/18 ,  C12Y101/01061 ,  C12Y102/01024 ,  C12Y103/99002

公开(公告)号：EP2330186B1

公开(公告)日：2014-12-31

申请号：EP09814823.2

申请日：2009-09-22

Applicant: Korea Advanced Institute of Science and Technology

Inventor： LEE, Sang Yup ,  XIA, Xiaoxia ,  QIAN, Zhi Gang ,  BAEK, Jong Hwan

IPC: C12N1/21 ,  C12N15/67 ,  C07K14/435

CPC classification number: C12N15/67 ,  C07K14/43518 ,  C07K14/43586