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    System for isolating and identifying leukocytes
    51.
    发明公开
    System for isolating and identifying leukocytes 失效
    System zur Isolierung und Identifizierung von Leukozyten。

    公开(公告)号：EP0627624A1

    公开(公告)日：1994-12-07

    申请号：EP94113130.2

    申请日：1988-03-11

    申请人： COULTER CORPORATION

    发明人： Ledis, Stephen ,  Crews, Harold R. ,  Fischer, Timothy J. ,  Sena, Ted

    IPC分类号： G01N33/50 ,  G01N15/12

    CPC分类号： G01N33/5094 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/108331

    摘要： A method and reagent system for the rapid isolation, identification, and/or analysis of leukocytes from a whole blood sample. The reagent system includes a lytic reagent solution comprising an aqueous solution of a water soluble compound which at least partially dissociates in aqueous solution to release a proton and a counterion and which, upon contact with a blood sample, acidifies the sample to a pH in the range of from about 2.6 to 4.0. The reagent system also includes a quenching reagent solution comprising an aqueous alkaline salt solution which effectively and rapidly arrests the chemical action of the lytic reagent solution on the sample and restores the native physiological environment of the sample. The method comprises using the reagent system to selectively lyse the red blood cells in a blood sample and modify the leukocytes to enable their differentiation into five sub-populations.

    摘要翻译： 用于从全血样品中快速分离，鉴定和/或分析白细胞的方法和试剂系统。 试剂系统包括裂解试剂溶液，其包含水溶性化合物的水溶液，其在水溶液中至少部分解离以释放质子和抗衡离子，并且在与血液样品接触时将样品酸化至pH 范围从2.6到4.0。 试剂系统还包括含有碱性盐水溶液的淬灭试剂溶液，其有效且快速地将溶解试剂溶液的化学作用阻止在样品上并恢复样品的天然生理环境。 该方法包括使用试剂系统选择性地裂解血液样品中的红细胞，并修饰白细胞使其能够分化成五个亚群。

    MULTI-PART DIFFERENTIAL ANALYZING APPARATUS UTILIZING LIGHT SCATTER TECHNIQUES
    52.
    发明授权
    MULTI-PART DIFFERENTIAL ANALYZING APPARATUS UTILIZING LIGHT SCATTER TECHNIQUES 失效
    MUCH PIECE差速器利用光散射技术的分析。

    公开(公告)号：EP0311655B1

    公开(公告)日：1994-11-30

    申请号：EP88903074.8

    申请日：1988-03-14

    申请人： COULTER CORPORATION

    发明人： RODRIGUEZ, Carlos, M. ,  COULTER, Wallace, H.

    IPC分类号： G01N33/48

    CPC分类号： G01N15/1459 ,  G01N1/38 ,  G01N21/53 ,  G01N2015/008 ,  G01N2015/1037 ,  G01N2015/1062 ,  G01N2015/129 ,  G01N2015/1413 ,  G01N2015/1477 ,  G01N2021/4711

    摘要： A particle analyzing method and apparatus for white blood cell (leukocyte) differentiation by optical and electronic measurements, without the use of dyes, on a flowing stream of leukocytes. A hydrodynamically focused stream of the leukocytes, in native condition, is passed into and through a beam (20) of radiant energy in a flow cell (10), whereby the beam is uniquely scattered by different ones of the subpopulations of the leukocytes. The scattered light impinges upon light collection means (24) disposed adjacent the flow cell. For eosinophil detection, the scattered light is collected at angular positions from about 10° to 70° relative to the axis (26) of the beam (20). Other of the leukocyte subpopulations are differentiated relative to the eosinophils and each other by optical and/or electronic measurements, especially including the Coulter Principle of particle analysis.

    METHOD AND APPARATUS FOR SCREENING MICROSCOPIC CELLS UTILIZING POLARIZED LIGHT SCATTER TECHNIQUES
    54.
    发明公开
    METHOD AND APPARATUS FOR SCREENING MICROSCOPIC CELLS UTILIZING POLARIZED LIGHT SCATTER TECHNIQUES 失效
    串联的方法和装置通知微观细胞中使用技术来传播偏振光。

    公开(公告)号：EP0574508A1

    公开(公告)日：1993-12-22

    申请号：EP92907838.0

    申请日：1992-02-14

    申请人： COULTER CORPORATION

    发明人： HUDSON, James, Carey ,  RODRIGUEZ, Carlos, M. ,  RUSSELL, Thomas

    IPC分类号： C12M1 ,  C12Q1 ,  G01N21 ,  G01N33

    CPC分类号： G01N33/56972

    摘要： Un procédé et un appareil pour effectuer le dépistage d'un ou de plusieurs groupes cellulaires donnés masqués par une population cellulaire, à l'aide d'au moins un paramètre de détection par lumière polarisée. Le groupe cellulaire a étudier tel qu'un sous-groupe d'une population de leucocytes est dénombré au moyen de microsphères auxquelles sont liés des anticorps monoclonaux pour modifier les caractéristiques détectées de la sous-population spécifiée de cellules en vue de différencier le groupe cellulaire étudié de la population cellulaire qui le dissimule. Ainsi, des populations cellulaires superposées peuvent être analysées.

    HEMATOLOGY CONTROL COMPOSITIONS FOR THREE POPULATIONS OF LEUKOCYTES, AND METHODS FOR THEIR PREPARATION AND USE IN WHOLE BLOOD CONTROL SYSTEMS
    58.
    发明授权
    HEMATOLOGY CONTROL COMPOSITIONS FOR THREE POPULATIONS OF LEUKOCYTES, AND METHODS FOR THEIR PREPARATION AND USE IN WHOLE BLOOD CONTROL SYSTEMS 失效
    白血病三种人群的血液学控制组合物及其制备和使用全血控制系统的方法

    公开(公告)号：EP0181393B1

    公开(公告)日：1992-09-09

    申请号：EP85902765.8

    申请日：1985-05-07

    申请人： COULTER CORPORATION

    发明人： CARVER, Franklin, Jay ,  GERULA, Theodore, John

    IPC分类号： G01N33/48

    CPC分类号： G01N33/96 ,  G01N2496/05 ,  Y10T436/101666 ,  Y10T436/105831 ,  Y10T436/106664 ,  Y10T436/107497 ,  Y10T436/108331

    摘要： This invention primarily is directed to a hematology reference control solution, the three separate white cell controll portions thereof consisting of three types of fixed red cells of determined size distribution for checking the operation of a particle analyzing instrument, including its predetermined lower and upper threshold settings for each class or subclass of leukocytes. For preparing a human granulocyte analogue, nurse shark erythrocytes are altered and fixed in a chilled solution to simulate in number, size and distribution the granulocytes in human whole blood. Such a granulocyte analogue is useful as a free-standing reference control, for determination of human granulocytes only, or can be comingled with reference controls for lymphocytes and mononuclear cells for use with multi-parameter instruments to give a trimodal leukocyte reference control, which itself can be included in a multiple-analysis hematology reference control, having components for also determining red blood cell and platelet parameters.

    INTERLABORATORY QUALITY ASSURANCE PROGRAM
    60.
    发明授权
    INTERLABORATORY QUALITY ASSURANCE PROGRAM 失效
    良好的质量保证计划

    公开(公告)号：EP0263870B1

    公开(公告)日：1992-08-19

    申请号：EP87903008.8

    申请日：1987-03-30

    申请人： COULTER CORPORATION

    发明人： ANDERSON, Frank, C. ,  TWEDT, Dean, E.

    IPC分类号： G01N15/00 ,  G06F15/20

    CPC分类号： G01N35/00871 ,  G01N35/00712 ,  G01N2035/00653 ,  G06F17/18 ,  G06F19/00 ,  Y02A90/22 ,  Y02A90/26

    摘要： An improved procedure for statistically evaluating the performance, precision and/or accuracy, of at least one analytical instrument in a pool of like or substantially like instruments by comparing the performance of the one instrument located in one laboratory with the performance of like instruments in other laboratories. The procedure includes the step of quantitatively analyzing a reference or control specimen of known constituents by each instrument in the pool and collecting the resultant data from the one instrument in the pool and of the pool instruments. The mean data from the one instrument and of the pool instruments are mathematically correlated in a series of statistical equations for computing a novel, sensitive index known as the "Instrument Performance Index" or IPI. This IPI can be tabulated and/or graphically displayed, preferably as a single point in a Cartesian coordinate system, whereby an operator can tell at a glance the performance level with respect to both precision and accuracy of the one instrument in the pool. The ultimate IPI is composed of a novel Coefficient of Variation Index (CVI), which is a valuable measure of instrument precision, and the Standard Deviation Index (SDI), which is a measure of performance accuracy. Both the CVI and SDI are derived values according to the invention and are capable of tabulation and display.