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    METHOD OF ERROR REDUCTION IN NUCLEIC ACID POPULATIONS
    52.
    发明公开
    METHOD OF ERROR REDUCTION IN NUCLEIC ACID POPULATIONS 有权
    方法在DNA种群减少错误的

    公开(公告)号：EP1483409A4

    公开(公告)日：2006-03-08

    申请号：EP03711383

    申请日：2003-02-28

    申请人： WISCONSIN ALUMNI RES FOUND

    发明人： SUSSMAN MICHAEL R ,  CERRINA FRANCESCO ,  BELSHAW PETER J ,  KAYSEN JAMES H ,  RICHMOND KATHRYN

    IPC分类号： C12N15/09 ,  C07H21/04 ,  C12N15/10 ,  C12P19/34 ,  C12Q1/68

    CPC分类号： C12N15/10 ,  C12N15/101

    摘要： A method is disclosed for the direct synthesis of double stranded DNA molecules of a variety of sizes and with any desired sequence. The DNA molecule to be synthesis is logically broken up into smaller overlapping DNA segments. A maskless microarray synthesizer is used to make a DNA microarray on a substrate in which each element or feature of the array is populated by DNA of a one of the overlapping DNA segments. The complement of each segment is also made in the microarray. The DNA segments are released from the substrate and held under conditions favoring hybridization of DNA, under which conditions the segments will hybridize to form duplexes. The duplexes are then separated using a DNA binding agent which binds to improperly formed DNA helixes to remove errors from the set of DNA molecules. The segments can then be hybridized to each other to assemble the larger target DNA sequence.

    ISOLATION OF NUCLEIC ACIDS
    56.
    发明授权
    ISOLATION OF NUCLEIC ACIDS 有权
    核酸分离

    公开(公告)号：EP1345952B1

    公开(公告)日：2004-11-24

    申请号：EP01270542.2

    申请日：2001-12-14

    申请人： DNA Research Innovations Limited

    发明人： BAKER, Matthew, John

    IPC分类号： C07H1/08 ,  C07C229/12 ,  C07C233/47 ,  C07C229/16 ,  C07D295/08

    CPC分类号： C12N15/101 ,  C12N15/1003 ,  C12N15/1006 ,  C12N15/1013

    摘要： The present invention relates to materials for extracting nucleic acid using charge switch methods which involve, at a first pH, bringing the sample into contact with a material which comprises an ionisable group, wherein the material has a positive charge at said first pH, such that nucleic acid is bound to the material; and releasing the nucleic acid at a second, higher, pH at which the charge on the material is negative, neutral or less positive. The release of the nucleic acid occurs under mild conditions and the ionisable group is provided by a biological buffer, a polyhydroxylated amine, a detergent or surfactant, a carbohydrate, a nucleic acid base, a heterocyclic nitrogen-containing compound, a monoamine, a biological dye, or a negatively ionisable group, the pKa of which is between about 3.0 and 7.0 and a metal oxide which is positively charged at said first pH, and optionally also at said second pH.

    METHODS FOR TWO-DIMENSIONAL CONFORMATION-DEPENDENT SEPARATION OF NON-CIRCULAR NUCLEIC ACIDS
    57.
    发明公开
    METHODS FOR TWO-DIMENSIONAL CONFORMATION-DEPENDENT SEPARATION OF NON-CIRCULAR NUCLEIC ACIDS 有权
    方法的二维分离构NICHTZIRKULÄRERNUCLEIC

    公开(公告)号：EP1476549A1

    公开(公告)日：2004-11-17

    申请号：EP03703010.3

    申请日：2003-02-25

    申请人： Lifeind EHF.

    发明人： JONSSON, Jon Johannes ,  THORMAR, Hans Guttormur ,  GUNNARSSON, Gudmundur H. ,  GUDMUNDSSON, Bjarki

    IPC分类号： C12N15/10 ,  C12Q1/68

    CPC分类号： C12N15/101

    摘要： Methods are provided for separating linear nucleic acid fragments based on their conformation. The methods are based on novel two-dimensional gel electrophoresis techniques. In one aspect the method comprises electrophoresing a sample of nucleic acids in a first dimension said sample through a gel matrix under a first set of pre-determined electrophoresis conditions; electrophoresing said gel matrix in a second dimension under a second set of electrophoresis conditions, such that linear nucleic acid fragments of equal length but having different conformation are separated; said first and second electrophoresis conditions are different, such that in one of said dimensions electrophoresis allows separation of linear nucleic acid fragments based on conformation and length, and in the other of said dimensions electrophoresis allows separation of the sample fragments based substantially on length. Said difference is preferably established with a chemical agent capable of reducing conformational differences between linear nucleic acids fragments.

    Isolation of nucleid acids
    58.
    发明公开
    Isolation of nucleid acids 审中-公开
    Nucleinsäure，隔离

    公开(公告)号：EP1473299A2

    公开(公告)日：2004-11-03

    申请号：EP04017291.8

    申请日：2001-12-14

    申请人： DNA Research Innovations Limited

    发明人： Baker, Matthew, John

    IPC分类号： C07H1/08 ,  C07C229/12 ,  C07C233/47 ,  C07C229/16 ,  C07D295/08

    CPC分类号： C12N15/101 ,  C12N15/1003 ,  C12N15/1006 ,  C12N15/1013

    摘要： The present invention relates to materials for extracting nucleic acid using charge switch methods which involve, at a first pH, bringing the sample into contact with a material which comprises an ionisable group, wherein the material has a positive charge at said first pH, such that nucleic acid is bound to the material; and releasing the nucleic acid at a second, higher, pH at which the charge on the material is negative, neutral or less positive. The release of the nucleic acid occurs under mild conditions and the ionisable group is provided by a biological buffer, a polyhydroxylated amine, a detergent or surfactant, a carbohydrate, a nucleic acid base, a heterocyclic nitrogen-containing compound, a monoamine, a biological dye, or a negatively ionisable group, the pKa of which is between about 3.0 and 7.0 and a metal oxide which is positively charged at said first pH, and optionally also at said second pH.

    摘要翻译： 本发明涉及使用电荷开关方法提取核酸的材料，该方法涉及在第一pH下使样品与包含可离子化基团的材料接触，其中所述材料在所述第一pH下具有正电荷，使得 核酸与材料结合; 并在第二个较高的pH下释放核酸，在该pH下，材料上的电荷是负的，中性的或较小的阳性。 核酸的释放在温和条件下发生，并且可电离基团由生物缓冲液，多羟基化胺，去污剂或表面活性剂，碳水化合物，核酸碱基，杂环含氮化合物，单胺，生物 染料或负电离基团，其pKa在约3.0和7.0之间，金属氧化物在所述第一pH和任选地还在所述第二pH下带正电荷。