Abstract:
The present invention concerns a spectrometer comprising means for producing a parallel beam from a light source whose spectrum is to be measured and directing it to an orientable grating (10) which produces a diffracted beam, means for focusing said diffracted beam to a detector (18) which measures the power of a selected line of the spectrum (1), said means comprising a reflector (12), and means for measuring the wavelength of the selected line. The means for measuring the wavelength comprise a reference light source producing a reference spectrum (3), comparison means for comparing the wavelength of the measured reference spectrum (1) with the stored reference spectrum (3), means for rotating the grating (10) providing a coarse positioning of the diffracted beam relatively to the detector (18) and measuring the grating position, and means for rotating the reflector (12) providing a fine positioning of the diffracted beam relatively to the detector (18) and measuring the reflector (12) position.
Abstract:
Optical systems that provide for simultaneous images and spectra from an object, such as a tissue sample, an industrial object such as a computer chip, or any other object that can be viewed with an optical system such as a microscope, endoscope, telescope or camera. In some embodiments, the systems provide multiple images corresponding to various desired wavelength ranges within an original image of the object, as well as, if desired, directional pointer(s) that can provide both an identification of the precise location from which a spectrum is being obtained, as well as enhancing the ability to point the device.
Abstract:
A hemispherical detector comprising a plurality of photodetectors (12) arranged in a substantially contiguous array, the array being substantially in the shape of a half-sphere, the half-sphere defining a closed end (50) and an open end (60), the open end (60) defining a substancially circular face. Also provided is a method for constructing a hemispherical detector comprising the steps of making a press mold of the desired shape of the hemispherical detector, pouring a material into the press mold to form a cast, finishing the cast to remove any defects, coating the cast with a coating material, and attaching a plurality of photodetectors to the cast.
Abstract:
A small-spot imaging, spectrometry instrument (Fig. 1) for measuring properties of a sample (27) has a polarization-scrambling element, such as a Lyot depolarizer (19), incorporatied between the polarization-introducing components of the system, such as the beamsplitter (17), and the microscope objective (21) of the system. The Lyot depolarizer varies polarization with wavelength. Sinusoidal perturbation in the resulting measured spectrum can be removed by data processing techniques or, if the depolarizer is thick or highly birefringent,l may be narrower than the wavelength resolution of the instrument.
Abstract:
An optical system having a first order spectral range that is usable in an optical spectrum analyzer receives an broadband optical test signal and a optical calibration signal and couples the optical signals via two optically isolated paths to separate optical detectors. First and second pairs of optical fibers, with each pair having an input fiber and an output fiber, are positioned in a focal plane of a collimating optic that has an optical axis. The fiber pairs are symmetrically positioned on either side of the optical axis with the input fibers positioned on one side of the optical axis and the output fibers positioned on the opposite side of the optical axis. The input fibers receive the optical test signal and the optical calibration signal. The output optical fibers are coupled to first and second optical detectors. An optical calibration source generates second order or greater spectral lines that fall within the first order spectral range of the optical system. A diffraction grating receives the optical test signal and the optical calibration signal from the collimating optic and separates the first order spectral components of the broadband optical test signal and passes the second order or greater spectral lines of the optical calibration signal. The first optical detector that is responsive to the first order spectral components of the optical test signal receives the optical test signal from the collimating optic and converts the optical test signal to an electrical signal. A second optical detector that is responsive to the second order or greater spectral lines of the optical calibration signal concurrently receives the optical calibration signal from the collimating optic and converts the calibrations signal to an electrical signal.
Abstract:
A solid state image pickup device (31) in which a plurality of picture elements are arranged in two dimensions to pick up the image of the distribution in two-dimensional space of radiative ray, light beams, electrons, ions, etc., and which is characterized by an opening (34) piercing a substrate (33b) being provided at the roughly center of an image pickup part (33) where the picture elements are arranged, and a charge transfer path to connect charge transfer electrodes (33d) to read out the image of each picture element with one another being wired, avoiding the opening (34). Furthermore, the image pickup part (33) is divided into at least two regions with the boundary between them lying partially in the opening (34), and each region has an exclusive register for charge readout. Hereby, this image pickup device doubles as an image pickup device to pick up the two-dimensional distribution of radiative ray, etc. and an aperture to this radiation.
Abstract:
A cassette reader (14) for detecting the status of stained samples (70) is provided. The colorimeter includes one or more high power emitting diodes (64a, 64b) as the source of illuminating the sample (70). An elliptical mirror (69), with a central aperture to permit the light to penetrate on to the sample (70), directs the scattered light on to its focal point at a detector (68). The colorimeter includes a microprocessor (62) which compares an output signal for the percent ratio value of the detected values of a sample (70) and a standard sample. The colorimeter housing (100) provides position sensors (18a-18d) which allow the identification of the well (16a-16d) being examined. Finally, the system is modified to measure cell motility by determining the ratio of motile sperms to total cell concentration.
Abstract:
A solid-state image sensor 31, which has a two-dimensional matrix of a plurality of pixels used to sense the two-dimensional spatial distribution of radioactive rays, light rays, electrons, ions, or the like, is provided with an aperture 34 that extends through a substrate 33b of an image sensing unit 33 on which the pixels are arranged, and a signal transfer path that connects signal transfer electrodes 33d for reading images of the respective pixels kept clear of the aperture 34. Furthermore, the image sensing unit 33 is divided into at least two regions by a boundary including the aperture 34, and these regions have individual signal read registers. With this structure, the image sensor serves as both an image sensor for sensing the two-dimensional distribution of radiation or the like, and an aperture for passing such radiation.
Abstract:
An electron microscope (10) is adapted to enable spectroscopic analysis of a sample (16). A parabolic mirror (18) has a central aperture (20) through which the electron beam can pass. The mirror (18) focuses laser illumination from a transverse optical path (24) onto the sample, and collects Raman and/or other scattered light, passing it back to an optical system (30). The mirror (18) is retractable (within the vacuum of the electron microscope) by a sliding arm assembly (22).
Abstract:
A new immunoassay system is provided for the detection of ligands or ligand binding partners in solution in a heterogeneous format. The invention relies upon the detection of back scattered light from an evanescent wave disturbed by the presence of a colloidal gold label brought to the interface by an immunological reaction. The evanescent wave existing at the interface in turn is the result of a totally internally reflected incident light wave. Placement of the detector at a back angle above the critical angle insures a superior signal-to-noise ratio. Apparatus and methods for scanning, detecting and manipulating light including a scattered total internal reflectance immunoassay system are provided.