公开(公告)号：EP1625203B1

公开(公告)日：2015-04-08

申请号：EP04752469.9

申请日：2004-05-15

申请人： XY, LLC

发明人： EVANS, Kenneth, M. ,  GILLIGAN, Thomas, B. ,  SUH, Tae Kwang ,  COX, Todd, A.

IPC分类号： G01N15/14 ,  G01N33/50 ,  C12N5/071

CPC分类号： C12Q3/00 ,  C12N5/0612 ,  G01N15/147 ,  G01N15/1475 ,  G01N33/5005 ,  G01N2015/149 ,  Y10T436/12

公开(公告)号：EP2743673A4

公开(公告)日：2015-03-18

申请号：EP13823849

申请日：2013-06-10

申请人： SONY CORP

发明人： MURAKI YOSUKE

IPC分类号： G01N15/14 ,  B01L3/00 ,  G01N1/00 ,  G01N15/10 ,  G01N37/00

CPC分类号： B01L3/502 ,  G01N15/1404 ,  G01N15/1459 ,  G01N2015/1006 ,  G01N2015/1409 ,  G01N2015/1413 ,  G01N2015/149

摘要： Provided is a microparticle measurement device that can deliver a liquid that is used in the analysis of microparticles in a stable manner. The microparticle measurement device includes a plurality of first tank units to which a liquid is supplied from the outside, and which are respectively connected in parallel, and a bulb unit that is connected to the plurality of first tank units, and which switches to a state in which it is possible to deliver the liquid to a flow channel through which microparticles flow. According to this microparticle measurement device, in addition to performing liquid delivery from a portion of first tank units, in which the replenishment of liquid has been completed, to a flow channel, it is possible to supply liquid from the outside to first tank units other than the first tank units that are delivering liquid.

摘要翻译： 本发明提供一种能够以稳定的方式输送用于微粒分析的液体的微粒测定装置。 微粒测量装置包括多个第一罐单元，从外部供给液体并且分别并联连接的第一罐单元和连接到多个第一罐单元的灯泡单元，并且切换到状态 其中可以将液体输送到微流体通过的流动通道。 根据该微粒测量装置，除了从液体的补充完成的第一罐单元的一部分进行液体输送到流路之外，还可以从外部向第一罐单元另外供给液体 比传送液体的第一个坦克单位。

公开(公告)号：EP2446246B1

公开(公告)日：2015-03-18

申请号：EP10726099.4

申请日：2010-06-24

申请人： Masterrind GmbH

发明人： MEYER, Heiko ,  LORBEER, Raoul, Amadeus ,  HEISTERKAMP, Alexander ,  RATH, Detlef

IPC分类号： G01N15/14

CPC分类号： G01N15/1459 ,  G01N2015/149

公开(公告)号：EP2732395A4

公开(公告)日：2015-03-11

申请号：EP12826436

申请日：2012-08-24

申请人： SONY CORP ,  SONY CORP AMERICA

发明人： DURACK GARY ,  FLEISCHER STEPHEN D ,  HATCHER JEREMY ,  ROBERTS DAVID ,  ZORDAN MICHAEL

IPC分类号： G06F19/00 ,  G01N15/14 ,  G06F19/10

CPC分类号： G01N15/1404 ,  G01N15/1427 ,  G01N15/1429 ,  G01N15/1459 ,  G01N2015/1006 ,  G01N2015/1075 ,  G01N2015/1415 ,  G01N2015/1438 ,  G01N2015/1477 ,  G01N2015/149

摘要： Apparatus and methods for detecting, characterizing, and compensating motion-related error of moving micro-entities are described. Motion-related error may occur in streams of moving micro-entities, and may represent a deviation in and expected arrival time or an uncertainty in position of a micro-entity within the stream. Motion-related error of micro-entities is observed in a flow cytometer, e.g., as pulse jitter, and is found to have a functional dependence on a parameter of the system. The pulse jitter can be compensated, according to one embodiment, by adjusting data acquisition observation windows. For the flow cytometer, a reduction of pulse jitter can improve measurement accuracy, resolution of doublets, system throughput, and enable an increase in an interrogation region for probing the micro-entities.

公开(公告)号：EP2270236B1

公开(公告)日：2015-03-04

申请号：EP10179420.4

申请日：2005-10-10

申请人： Medical Research Council ,  The President and Fellows of Harvard College

发明人： Griffiths, Andrew ,  Weitz, David ,  Link, Darren ,  Ahn, Keunho ,  Bibette, Jerome

IPC分类号： C12Q1/68 ,  C12N15/09 ,  B01F13/00 ,  B01F3/08

CPC分类号： B01F3/0807 ,  B01F5/0646 ,  B01F5/0647 ,  B01F5/0655 ,  B01F13/0062 ,  B01F13/0071 ,  B01F13/0076 ,  B01J19/0046 ,  B01J2219/00466 ,  B01J2219/00468 ,  B01J2219/005 ,  B01J2219/0052 ,  B01J2219/00545 ,  B01J2219/00576 ,  B01J2219/00657 ,  B01J2219/00722 ,  B01L3/502746 ,  B01L3/502753 ,  B01L3/502776 ,  B01L3/502784 ,  B01L2200/0636 ,  B01L2200/0673 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0415 ,  B01L2400/0487 ,  C12N15/1058 ,  C12N15/1075 ,  C12P21/00 ,  C12Q1/6874 ,  G01N15/1459 ,  G01N15/1484 ,  G01N2015/149

摘要： The invention describes a method for isolating one or more genetic elements encoding a gene product having a desire activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; and (b) sorting the genetic elements which express the gene product having the desired activity; wherein at least one step is under microfluidic control. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention.

公开(公告)号：EP2801813A1

公开(公告)日：2014-11-12

申请号：EP14172013.6

申请日：2006-02-01

申请人： Premium Genetics (UK) Limited

发明人： Mueth, Daniel ,  Anderson, Amy ,  Knutson, Christopher ,  Plewa, Joseph

IPC分类号： G01N21/01 ,  G01N33/00 ,  G01N15/14

CPC分类号： G01N21/6428 ,  C12M47/04 ,  G01N15/1404 ,  G01N15/1459 ,  G01N2015/1409 ,  G01N2015/1411 ,  G01N2015/149

摘要： An apparatus for sorting and orienting sperm cells includes a pair of walls in confronting arrangement and forming a flow chamber having an inlet, a downstream outlet, and an intermediate detector region. The inlet receives first and second spaced apart streams of input fluid and a third stream of sample fluid containing the cells to be sorted. The first and second streams have respective flow rates relative to the third stream, such that the third stream is constricted, forming a relatively narrow sample stream, so that the cells are oriented parallel to the walls. A detector detects the desired cells and a sorter downstream of the detector, sorts the desired cells from the stream.

公开(公告)号：EP2798393A1

公开(公告)日：2014-11-05

申请号：EP12808249.2

申请日：2012-12-28

申请人： Danmarks Tekniske Universitet

发明人： GLÜCKSTAD, Jesper

IPC分类号： G02B21/32 ,  G01N15/00 ,  G21K1/00

CPC分类号： B03B7/00 ,  B01L3/502761 ,  B01L3/502776 ,  B01L2200/0668 ,  B01L2400/043 ,  G01N15/00 ,  G01N15/1459 ,  G01N15/1484 ,  G01N2015/149 ,  G02B21/32 ,  G21K1/006

摘要： There is presented a system 10,100 for sorting microscopic objects 76, 78, 80, where the system comprises a fluid channel 66 with an inlet 68 and an outlet 70, where the fluid channel is arranged for allowing the fluid flow to be laminar. The system furthermore comprises a detection system 52 which enables detecting microscopic objects in the fluid channel and furthermore enables determining their position. The system furthermore comprises a controller 67, such as a computer, which receives the positions and accordingly controls a source of light beams so as to "shoot" light beams towards selected microscopic objects so as to "push" them into a new position. The system thereby enables sorting the selected microscopic objects. In more specific embodiments, the detection system furthermore assigns different categories to different microscopic objects, so as to enable sorting based on multiple categories.

公开(公告)号：EP2796854A2

公开(公告)日：2014-10-29

申请号：EP14159719.5

申请日：2014-03-14

申请人： Cytonome/ST, LLC

发明人： Sharpe, Johnathan ,  Perrault, Donald

IPC分类号： G01N15/14 ,  G01N15/10

CPC分类号： G01N15/1434 ,  G01N15/1459 ,  G01N15/1484 ,  G01N21/64 ,  G01N2015/1006 ,  G01N2015/1081 ,  G01N2015/149 ,  G02B3/0037 ,  G02B3/0087 ,  G02B5/09

摘要： The present disclosure provides improved optical systems for particle processing (e.g., cytometry including microfluidic based sorters, drop sorters, and/or cell purification) systems and methods. More particularly, the present disclosure provides advantageous micro-lens array optical detection assemblies for particle (e.g., cells, microscopic particles, etc.) processing systems and methods (e.g., for analyzing, sorting, processing, purifying, measuring, isolating, detecting, monitoring and/or enriching particles.

摘要翻译： 本公开提供用于颗粒处理的改进的光学系统（例如，包括基于微流体的分选机，滴定分选机和/或细胞纯化的细胞计数）系统和方法。 更具体地，本公开提供了用于颗粒（例如，细胞，微观颗粒等）的有利的微透镜阵列光学检测组件的处理系统和方法（例如，用于分析，分选，加工，净化，测量，隔离，检测， 监测和/或富集颗粒。

公开(公告)号：EP2786118A1

公开(公告)日：2014-10-08

申请号：EP13733886.9

申请日：2013-01-04

申请人： Sony Corporation ,  Sony Corporation Of America

发明人： ZORDAN, Michael, D. ,  DURACK, Gary P. ,  YAMADA, Shinji ,  NARAHARI, Bharath K. ,  EATON, Elizabeth C. ,  WALLACE, Jeffrey D. ,  ARNOLD, Larry W. ,  KEILBACH, Kevin A.

IPC分类号： G01N15/14

CPC分类号： G01N21/53 ,  G01N15/1434 ,  G01N15/1459 ,  G01N21/645 ,  G01N2015/008 ,  G01N2015/1006 ,  G01N2015/149 ,  G01N2021/4711

摘要： The various embodiments disclosed herein utilize multiple lasers that have different wavelengths and a single detection path. The lasers are mounted orthogonal to one another so that one laser will provide a forward angle light scatter (FALS) signal in the detection path, and one laser will provide a side scatter signal in the detection path (i.e., the single detection optics are approximately in-line with the FALS laser and approximately orthogonal to the side scatter laser), The single detector path spectrally separates the forward and side scatter signals prior to applying them to their respective detectors for measurement,

公开(公告)号：EP2270235B1

公开(公告)日：2014-10-01

申请号：EP10179400.6

申请日：2005-10-10

申请人： Medical Research Council ,  The President and Fellows of Harvard College

发明人： Griffiths, Andrew ,  Weitz, David ,  Link, Darren ,  Ahn, Keunho ,  Bibette, Jerome

IPC分类号： C12Q1/68 ,  C12N15/09 ,  B01F13/00 ,  B01F3/08

CPC分类号： B01F3/0807 ,  B01F5/0646 ,  B01F5/0647 ,  B01F5/0655 ,  B01F13/0062 ,  B01F13/0071 ,  B01F13/0076 ,  B01J19/0046 ,  B01J2219/00466 ,  B01J2219/00468 ,  B01J2219/005 ,  B01J2219/0052 ,  B01J2219/00545 ,  B01J2219/00576 ,  B01J2219/00657 ,  B01J2219/00722 ,  B01L3/502746 ,  B01L3/502753 ,  B01L3/502776 ,  B01L3/502784 ,  B01L2200/0636 ,  B01L2200/0673 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0415 ,  B01L2400/0487 ,  C12N15/1058 ,  C12N15/1075 ,  C12P21/00 ,  C12Q1/6874 ,  G01N15/1459 ,  G01N15/1484 ,  G01N2015/149

摘要： The invention describes a method for isolating one or more genetic elements encoding a gene product having a desire activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; and (b) sorting the genetic elements which express the gene product having the desired activity; wherein at least one step is under microfluidic control. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention.