公开(公告)号：EP2810067A2

公开(公告)日：2014-12-10

申请号：EP13742955.1

申请日：2013-01-31

申请人： American University of Cairo (AUC)

发明人： AZZAZY, Hassan Mohamed El-said ,  SHAWKY ABDUO, Sherif Mohamed ,  EID, Kamel Abdelmenem Mohamed

IPC分类号： G01N33/50 ,  G01N33/553 ,  C12Q1/68 ,  G01N33/68

CPC分类号： A01N59/16 ,  B22F1/0003 ,  B22F9/20 ,  C12Q1/6816 ,  C12Q1/70 ,  G01N33/5308 ,  G01N33/552 ,  G01N33/553 ,  G01N33/587 ,  Y10T436/143333 ,  C12Q2523/31 ,  C12Q2563/155 ,  C12Q2565/628

摘要： A gold nanoparticle-based assay for the detection of a target molecule, such as Hepatitis C Virus (HCV) RNA in serum samples, that uses positively charged gold nanoparticles (AuNPs) in solution based format. The assay has been tested on 74 serum clinical samples suspected of containing HCV RNA, with 48 and 38 positive and negative samples respectively. The developed assay has a specificity and sensitivity of 96.5% and 92.6% respectively. The results obtained were confirmed by Real-Time PCR, and a concordance of 100% for the negative samples and 89% for the positive samples has been obtained between the Real-Time PCR and the developed AuNPs based assay. Also, a purification method for the HCV RNA has been developed using HCV RNA specific probe conjugated to homemade silica nanoparticles. These silica nanoparticles have been synthesized by modified Stober method. This purification method enhanced the specificity of the developed AuNPs assay. The method can detect a target molecule, such as HCV RNA in serum, by employing modified silica nanoparticles to capture the target from a biological sample followed by detection of the captured target molecule using positively charged AuNPs. The assay is simple, cheap, sensitive and specific. Another aspect of the invention is anisotropic silver nanoparticles and methods of their use.

公开(公告)号：EP2806736A1

公开(公告)日：2014-12-03

申请号：EP12841851.4

申请日：2012-10-19

申请人： Omthera Pharmaceuticals, Inc. ,  Davidson, Michael H. ,  Wisler, Gerald L.

发明人： DAVIDSON, Michael H. ,  WISLER, Gerald L.

IPC分类号： A01N37/00

CPC分类号： G01N33/92 ,  A23L33/12 ,  A61K9/006 ,  A61K31/202 ,  A61K31/232 ,  A61K45/06 ,  C12Q1/26 ,  C12Q1/6883 ,  C12Q2600/156 ,  G01N2800/305 ,  G01N2800/50 ,  Y10T436/143333 ,  A61K2300/00

摘要： The present disclosure relates to methods of identifying subjects who are suffering from, or are susceptible to developing, ADHD. The methods comprise determining whether the subject is an efficient converter of medium chain polyunsaturated fatty acids to long-chain polyunsaturated fatty acids. Also provided are methods of treating ADHD in a subject, comprising administering to the subject an effective amount of a composition comprising long chain omega-3 fatty acids.

公开(公告)号：EP2805157A2

公开(公告)日：2014-11-26

申请号：EP13704843.5

申请日：2013-01-18

申请人： Life Technologies Corporation ,  Li, Shifeng ,  Bustillo, James ,  Hinz, Wolfgang

发明人： LI, Shifeng ,  BUSTILLO, James ,  HINZ, Wolfgang

IPC分类号： G01N27/414

CPC分类号： G01N27/4145 ,  C12Q1/6869 ,  G01N27/414 ,  Y10T436/143333

摘要： A system includes a sensor including a sensor pad and a well wall structure defining a well operatively coupled to the sensor pad. The well is further defined by a lower surface disposed over the sensor pad. The well wall structure defines an upper surface and defines a wall surface extending between the upper surface and the lower surface. The system further includes a conductive layer disposed over the lower surface and the wall surface.

摘要翻译： 一种系统包括传感器，其包括传感器垫和井壁结构，该结构限定了可操作地耦合到传感器垫的井。 该阱还由设置在传感器垫上的下表面限定。 井壁结构限定了上表面并限定了在上表面和下表面之间延伸的壁表面。 该系统还包括设置在下表面和壁表面上的导电层。

公开(公告)号：EP2784508A2

公开(公告)日：2014-10-01

申请号：EP14173968.0

申请日：2009-12-25

申请人： Universal Bio Research Co., Ltd.

发明人： Tajima, Hideji

IPC分类号： G01N33/543 ,  G01N1/28 ,  G01N33/553 ,  G01N30/02 ,  C12Q1/68

CPC分类号： G01N33/54386 ,  B01L3/508 ,  B01L3/52 ,  B01L2200/10 ,  B01L2300/0636 ,  C12Q1/6804 ,  G01N33/54326 ,  G01N33/54393 ,  G01N35/026 ,  G01N35/1009 ,  Y10T436/143333 ,  Y10T436/25125 ,  C12Q2563/143

摘要： A biologically-relevant substance assay system is disclosed. In a disclosed embodiment, the system comprises (a) a specimen holding portion in which a specimen is held; (b) a first holding portion in which trapping particles for trapping a biologically-relevant substance from the specimen are held; (c) a second holding portion in which a reagent for detecting the biologically-relevant substance is held; and (d) a dispensing mechanism for dispensing the specimen into the specimen holding portion, a mechanism for mixing the specimen with the trapping particles to extract the biologically-relevant substance from the specimen, and a mechanism for mixing the extracted biologically-relevant substance with the reagent for detecting; wherein the specimen holding portion, the first holding portion, and the second holding portion are arranged in an approximate straight line.

摘要翻译： 公开了生物相关物质测定系统。 在公开的实施例中，该系统包括：（a）保持样本的样本保持部; （b）第一保持部分，其中保持用于捕获来自所述样本的生物相关物质的捕获颗粒; （c）保持用于检测生物相关物质的试剂的第二保持部分; （d）将试样分注到试样保持部的分注机构，将试样与捕获粒子混合而从试样中提取生物体相关物质的机构，以及将提取出的生物体相关物质与 用于检测的试剂; 其中所述样本保持部分，所述第一保持部分和所述第二保持部分布置成近似直线。

公开(公告)号：EP1352971B1

公开(公告)日：2014-09-17

申请号：EP03002612.4

申请日：1996-03-18

申请人： Agena Bioscience, Inc.

发明人： Köster, Hubert

IPC分类号： C12Q1/68 ,  C12Q1/70

CPC分类号： C12Q1/6872 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6837 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6862 ,  C12Q1/6883 ,  C12Q1/706 ,  G01N35/1067 ,  H01J49/00 ,  Y10T436/143333 ,  Y10T436/24 ,  C12Q2565/627 ,  C12Q2537/113 ,  C12Q2535/113 ,  C12Q2521/325 ,  C12Q2535/131 ,  C12Q2525/107 ,  C12Q2523/107 ,  C12Q2523/319 ,  C12Q2565/625 ,  C12Q2535/125

公开(公告)号：EP2684041A4

公开(公告)日：2014-09-10

申请号：EP12754284

申请日：2012-02-27

申请人： ARCHER DANIELS MIDLAND CO

发明人： HOWARD STEPHEN J ,  HAGBERG ERIK ,  ROCKAFELLOW ERIN

IPC分类号： G01N33/00 ,  G01N30/02

CPC分类号： G01N33/98 ,  G01N30/88 ,  G01N33/0013 ,  G01N33/50 ,  G01N2030/884 ,  Y10T436/143333

公开(公告)号：EP1910416B1

公开(公告)日：2014-09-10

申请号：EP05763929.6

申请日：2005-07-22

申请人： Erasmus University Medical Center Rotterdam

发明人： ESSERS, Jeroen ,  GARINIS, Georgios Aris ,  KANAAR, Roland

IPC分类号： C07K14/78 ,  C12N15/85

CPC分类号： C07K14/78 ,  A01K67/0276 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/0375 ,  C12N15/8509 ,  Y10T436/143333

公开(公告)号：EP2749867A1

公开(公告)日：2014-07-02

申请号：EP12827023.8

申请日：2012-07-26

申请人： Olympus Corporation

发明人： TANABE, Tetsuya ,  YAMAGUCHI, Mitsushiro

IPC分类号： G01N21/64

CPC分类号： G01N21/64 ,  G01N21/6408 ,  G01N21/645 ,  G01N21/6452 ,  G01N21/6458 ,  G01N21/6486 ,  G02B21/0032 ,  G02B21/0048 ,  G02B21/0076 ,  Y10T436/143333

摘要： There is provided an optical analysis technique of detecting light of a light-emitting particle in a sample solution in the scanning molecule counting method using the light measurement with a confocal microscope or a multiphoton microscope, for suppressing the scattering in detected results of signals of light of light-emitting particles smaller and achieving the improvement of accuracy. The inventive optical analysis technique is characterized by moving the position of a light detection region of the optical system of the microscope along a predetermined route at least two circulation times by changing the optical path of the optical system; detecting light from the light detection region and generating time series light intensity data during the moving of the position of the light detection region along the predetermined route of multiple times and detecting individually a signal indicating light from each light-emitting particle existing in the predetermined route using the time series light intensity data obtained in the circulating movement of the light detection region along the predetermined route of multiple times.

摘要翻译： 提供了一种使用共聚焦显微镜或多光子显微镜的光测量的扫描分子计数法中的样品溶液中的发光粒子的光的光学分析技术，用于抑制光的信号的检测结果中的散射 的发光粒子较小，实现了精度的提高。 本发明的光学分析技术的特征在于通过改变光学系​​统的光路，沿着预定路线移动显微镜的光学系统的光检测区域的位置至少两个循环时间; 检测来自光检测区域的光，并且在沿着预定路线多次移动光检测区域期间产生时间序列光强度数据，并分别检测指示存在于预定路线中的每个发光粒子的光的信号 使用沿着预定路线多次的光检测区域的循环移动中获得的时间序列光强度数据。

公开(公告)号：EP2227564B1

公开(公告)日：2014-07-02

申请号：EP08859169.8

申请日：2008-11-24

申请人： Perkinelmer LAS, Inc.

发明人： ADLER, Karl Edwin ,  SCHERMER, Mack J.

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6811 ,  C12Q1/6809 ,  C12Q1/6813 ,  C12Q1/6876 ,  Y10T436/143333 ,  C12Q2563/149 ,  C12Q2537/143 ,  C12Q2531/113 ,  C12Q2525/179 ,  C12Q2539/115

公开(公告)号：EP0938320B2

公开(公告)日：2014-06-18

申请号：EP97915870.6

申请日：1997-03-14

申请人： Kopreski, Michael, S.

发明人： Kopreski, Michael, S.

IPC分类号： A61K31/70 ,  A61K39/395 ,  C12P19/34 ,  C12Q1/68 ,  C12Q1/70

CPC分类号： C12Q1/6806 ,  C12Q1/6886 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16 ,  Y10T436/143333 ,  C12Q2521/119