利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

116 条记录 (耗时 0.018 秒)

    VORRICHTUNG UND VERFAHREN ZUM ANORDNEN VON PIPETTEN- ODER DISPENSER-SPITZEN IN EINEM SYSTEM ZUM MANIPULIEREN VON FLÜSSIGKEITSPROBEN
    3.
    发明公开
    VORRICHTUNG UND VERFAHREN ZUM ANORDNEN VON PIPETTEN- ODER DISPENSER-SPITZEN IN EINEM SYSTEM ZUM MANIPULIEREN VON FLÜSSIGKEITSPROBEN 有权
    装置和方法为系统中的操纵液体样品的移液管或分配器TIPS配售

    公开(公告)号：EP1759215A1

    公开(公告)日：2007-03-07

    申请号：EP05742111.7

    申请日：2005-05-30

    申请人： Tecan Trading AG

    发明人： WIGGLI, Markus ,  SCHINZEL, Fred ,  FORSTER, Bronwen

    IPC分类号： G01N35/10

    CPC分类号： G01N35/1067 ,  Y10T436/2575

    摘要： Disclosed are devices (1) and methods for disposing pipette syringes or dispenser syringes (2) in a system (3) used for manipulating liquid samples (4). One inventive device (1) comprises: a robot (5) for orienting pipette syringes or dispenser syringes (2) relative to sample receptacles (6) located in or on the system (3) in an X direction and in a Y direction that extends substantially at a right angle thereto; pipette syringes or dispenser syringes (2) which extend essentially in a vertical direction and can be raised and lowered in a Z direction running substantially perpendicular to the X and Y directions; drive units for moving the robot (5); and processors for controlling the movements and actions of the robot (5) and the pipette syringes and dispenser syringes (2). The inventive device (1) is characterized in that the same comprises at least two blocks (8, 9) which are arranged on a robot (5) arm (7) and on which at least two pipette syringes or dispenser syringes (2) are placed at an axial distance (10) from each other. The axial distance (10) essentially corresponds to the grid space of wells (19) of a microplate (18). At least one of said blocks (8, 9) is configured so as to be individually movable in the X direction and Y direction in order to alternately orient the pipette syringes or dispenser syringes (2) of the two blocks (8, 9) in a staggered manner along a common line (12). The disclosed method makes it possible to re-pipette liquid sample (4) in a time-saving fashion between several sample receptacles, e.g. between small sample tubes and microplates (18) encompassing 24, 96, 384, or 1536 wells.

    Dna sequencing by mass spectrometry
    10.
    发明公开
    Dna sequencing by mass spectrometry 失效
    Dns-Sequenzierung durch Massenspektrometrie

    公开(公告)号：EP1262564A2

    公开(公告)日：2002-12-04

    申请号：EP02016384.6

    申请日：1994-01-06

    申请人： SEQUENOM, INC.

    发明人： Köster, Hubert

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6827 ,  C12Q1/6816 ,  C12Q1/6872 ,  G01N35/1067 ,  Y10S435/81 ,  Y10T436/143333 ,  Y10T436/24 ,  Y10T436/25 ,  C12Q2565/627 ,  C12Q2563/167 ,  C12Q2537/113 ,  C12Q2537/143

    摘要： The invention describes a new method to sequence DNA. The improvements over the existing DNA sequencing technologies are high speed, high throughput, no electrophoresis and gel reading artifacts due to the complete absence of an electrophoretic step, and no costly reagents involving various substitutions with stable isotopes. The invention utilizes the Sanger sequencing strategy and assembles the sequence information by analysis of the nested fragments obtained by base-specific chain termination via their different molecular masses using mass spectrometry, as for example, MALDI or ES mass spectrometry. A further increase in throughput can be obtained by introducing mass-modifications in the oligonucleotide primer, chain-terminating nucleoside triphosphates and/or in the chain-elongating nucleoside triphosphates, as well as using integrated tag sequences which allow multiplexing by hybridization of tag specific probes with mass-differentiated molecular weights.

    摘要翻译： 本发明描述了一种新的DNA序列化方法。 由于完全不存在电泳步骤，现有DNA测序技术的改进是高速度，高通量，无电泳和凝胶读数伪像，并且没有昂贵的试剂涉及具有稳定同位素的各种取代。 本发明利用Sanger测序策略，并通过使用质谱法，例如MALDI或ES质谱法，通过分析通过其不同分子量通过碱基特异性链终止获得的嵌套片段来组装序列信息。 通过在寡核苷酸引物，链终止的三磷酸核苷和/或在延伸链的三磷酸的链中引入质量修饰，以及使用允许通过标签特异性探针的杂交进行多重复合的整合的标签序列，可以获得通量的进一步增加 具有质量分化的分子量。