公开(公告)号：EP2210947B1

公开(公告)日：2013-07-17

申请号：EP10157183.4

申请日：1993-11-30

申请人： Icahn School of Medicine at Mount Sinai

发明人： Desnick, Robert J. ,  Bishop, David F. ,  Ioannou, Yiannis A.

IPC分类号： C12N15/56 ,  C12N15/54 ,  C12N15/62 ,  C12N9/40 ,  C12N9/10 ,  C12N9/24

CPC分类号： C12N9/2465 ,  A61K35/18 ,  A61K38/00 ,  C07K14/31 ,  C07K2319/00 ,  C07K2319/61 ,  C07K2319/705 ,  C12N9/1081 ,  C12Y302/01022 ,  C12Y302/01049

摘要： The invention discloses a method for producing secreted proteins, comprising the steps of (a) culturing a transformed mammalian cell containing a nucleotide sequence encoding the secreted protein operatively associated with a nucleotide sequence that regulates gene expression; and (b) isolating said protein from the mammalian cell culture.

公开(公告)号：EP2558484A1

公开(公告)日：2013-02-20

申请号：EP11768414.2

申请日：2011-04-11

申请人： Novozymes A/S

发明人： LI, Ming ,  DUAN, Junxin ,  LIU, Zheng ,  FUKUYAMA, Shiro ,  AYABE, Keiichi ,  COWARD-KELLY, Guillermo ,  DEINHAMMER, Randall

IPC分类号： C07K14/385 ,  C12N9/34 ,  C12N15/56 ,  C12N15/63 ,  C12N1/00 ,  C12P19/20 ,  C12P7/00 ,  C12R1/80

CPC分类号： C12P19/14 ,  C12N9/2428 ,  C12P7/06 ,  C12P19/02 ,  C12P19/20 ,  Y02E50/17

摘要： Provided are isolated polypeptides having glucoamylase activity and isolated polynucleotides encoding the polypeptides. Also provided are nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

公开(公告)号：EP2535415A1

公开(公告)日：2012-12-19

申请号：EP10845466.1

申请日：2010-02-10

申请人： Yuen Foong Yu Paper Mfg Co. Ltd.

发明人： HUANG, Yahui ,  HSIEH, Chunhui

IPC分类号： C12N15/56 ,  C12N9/24 ,  C12N9/42 ,  C12N15/63 ,  C12N15/81

CPC分类号： C12N9/2482 ,  C12Y302/01008

摘要： Modified nucleotide molecules of xylanase and the application of the nucleotide molecules in constructing recombinant vectors, host cells or producing xylanase are disclosed, wherein the nucleotide molecules contain nucleotide sequences having greater than 80% identity with nucleotide sequence shown by SEQ ID NO: 1.

摘要翻译： 公开了木聚糖酶的修饰的核苷酸分子和构建重组载体，宿主细胞或产生木聚糖酶的核苷酸分子的应用，其中核苷酸分子含有与SEQ ID NO：1所示的核苷酸序列具有大于80％同一性的核苷酸序列。

公开(公告)号：EP2428572A3

公开(公告)日：2012-12-12

申请号：EP11192225.8

申请日：2008-03-04

申请人： Danisco US, Inc., Genencor Division

发明人： Chang, Claudine ,  De Nobel, Hans ,  Jones, Brian E. ,  Naab, Corey ,  Kolkman, Marc ,  Vroemen, Casper ,  Weyler, Walter

IPC分类号： C12N15/56 ,  C12N9/28 ,  C12P19/14 ,  A23L1/305 ,  C11D3/386 ,  D06M16/00

CPC分类号： C11D3/38609 ,  C11D3/38618 ,  C12N9/2417 ,  C12P19/14 ,  C12Y302/01001 ,  D06M16/003

摘要： Disclosed are variants of the α-amylase derived from Bacillus sp. no. 707, compositions comprising said variants, compositions comprising the variants, and methods of using the variants. The methods of use include methods of cleaning surfaces, laundering textiles, desizing, hydrolyzing biofilms of various substrates, and treating starch (e.g., liquefaction and saccharification).

公开(公告)号：EP1516053B1

公开(公告)日：2012-10-17

申请号：EP03760440.2

申请日：2003-06-16

申请人： Verenium Corporation ,  BP Corporation North America Inc.

发明人： STEER, Brian ,  CALLEN, Walter ,  HEALEY, Shaun ,  HAZLEWOOD, Geoff ,  WU, Di ,  BLUM, David ,  ESTEGHLALIAN, Alireza

IPC分类号： C12N15/56 ,  C12N9/24 ,  C12N15/09 ,  C12N15/11 ,  C12N15/63 ,  C12N1/21 ,  C12N5/10

CPC分类号： C12N9/248 ,  A01K2217/05 ,  C07K2319/00 ,  C07K2319/02 ,  C12N9/2434 ,  C12N9/2482 ,  C12P19/14 ,  C12Y302/01008 ,  D21C5/005 ,  D21H17/005 ,  Y02E50/16 ,  Y02E50/17

摘要： Xylanases, Nucleic Acids Encoding Them and Methods for Making and Using Them The invention relates to xylanases and to polynucleotides encoding the xylanases. In addition, methods of designing new xylanases and methods of use are also provided. The xylanases have increased activity and stability at increased pH and temperature.

公开(公告)号：EP2401369A4

公开(公告)日：2012-08-15

申请号：EP10746947

申请日：2010-02-26

申请人： CODEXIS INC

发明人： BAIDYAROY DIPNATH ,  CLARK LOUIS ,  NEWMAN LISA M ,  CHING CHARLENE

IPC分类号： C12N9/24 ,  C12N15/56 ,  C12P7/04 ,  C12P19/02

CPC分类号： C12N9/2445 ,  C12Y302/01021

公开(公告)号：EP2479265A1

公开(公告)日：2012-07-25

申请号：EP12164788.7

申请日：2008-04-08

申请人： Danisco US Inc.

发明人： Aehle, Wolfgang ,  Bott, Richard R. ,  Nikolaev, Igor ,  Scheffers, Martijn ,  van Solingen, Piet ,  Vroemen, Casper

IPC分类号： C12N9/24 ,  C12N9/34 ,  C07K14/37 ,  C12N15/56 ,  C12N15/31 ,  C12R1/885 ,  C12N15/62

CPC分类号： C12N9/2428 ,  Y02E50/17

摘要： The present invention relates to variant glucoamylases wherein the variant has altered properties (e.g., improved thermostability and/or specific activity) compared to a corresponding parent glucoamylase. The present invention also relates to enzyme compositions comprising a variant glucoamylase (e.g., starch hydrolysing compositions); DNA constructs comprising polynucleotides encoding the variants and methods of producing the variant glucoamylases in host cells.

摘要翻译： 本发明涉及变体葡糖淀粉酶，其中所述变体与相应的亲本葡糖淀粉酶相比具有改变的性质（例如改善的热稳定性和/或比活性）。 本发明还涉及包含变体葡糖淀粉酶（例如淀粉水解组合物）的酶组合物; 包含编码变体的多核苷酸的DNA构建体和在宿主细胞中产生变体葡糖淀粉酶的方法。

公开(公告)号：EP1733033B1

公开(公告)日：2012-06-20

申请号：EP05726395.6

申请日：2005-02-04

申请人： Novozymes Inc.

发明人： DOTSON, William ,  GREENIER, Jennifer ,  DING, Hanshu

IPC分类号： C12N9/42 ,  C12P1/02 ,  C12N15/56

CPC分类号： C07K14/37 ,  C11D3/38645 ,  C12N9/2445 ,  C12N15/8246 ,  C12P7/10 ,  C12Y302/01004 ,  C12Y302/01021 ,  C12Y302/01091 ,  Y02E50/16 ,  Y02E50/17

摘要： The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated nucleic acids encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acids as well as methods for producing and using the polypeptides.

公开(公告)号：EP2279241A4

公开(公告)日：2012-05-30

申请号：EP09743560

申请日：2009-05-06

申请人： ARCHER DANIELS MIDLAND CO

发明人： ABBAS CHARLES ,  SIBIRNY ANDRIY ,  VORONOVSKY ANDRIY Y

IPC分类号： C12N1/19 ,  C12N15/56 ,  C12P7/06 ,  C12R1/01

CPC分类号： C12N9/2428 ,  C12N9/2414 ,  C12N9/242 ,  C12P7/06 ,  C12Y302/01001 ,  C12Y302/01003 ,  C12Y302/01008 ,  C12Y302/01037 ,  C12Y401/01001 ,  Y02E50/17 ,  Y02P20/52

摘要： Genes SWA2 and GAM1 from the yeast, Schwanniomyces occidentalis, encoding α-amylase and glucoamylase, respectively, were cloned and expressed in H. polymorpha. The expression was achieved by integration of the SWA2 and GAM1 genes into the chromosome of H. polymorpha under operably linked to a strong constitutive promoter of the H. polymorpha-glyceraldehyde-3-phosphate dehydrogenase gene (HpGAP. Resulting transformants acquired the ability to grow on a minimal medium containing soluble starch as a sole carbon source and can produce Ethanol at high-temperature fermentation from starch up to 10 g/L. A XYN2 gene encoding endoxylanase was obtained from the fungus Trichoderma resee, and a xlnD gene coding for &bgr;-xylosidase was obtained from the fungus Aspergillus niger. Co-expression of these genes was also achieved by integration into the H. polymorpha chromosome under control of the HpGAP promoter. The resulting transformants were capable of growth on a minimal medium supplemented with birchwood xylan as a sole carbon source. Successful expression of xylanolytic enzymes resulted in a recipient strain capable of fermentation of birchwood xylan to ethanol at 48° C. Further with co expression of the forgoing genes in a H. polymorpha strain that overexpresses a pyruvate decarboxylase gene further improved ethanol production.

公开(公告)号：EP2007884B1

公开(公告)日：2012-05-16

申请号：EP07730683.5

申请日：2007-04-12

申请人： AB Enzymes Oy

发明人： ALAPURANEN, Marika ,  VALTAKARI, Leena ,  KALLIO, Jarno ,  OJAPALO, Pentti ,  VEHMAANPERÄ, Jari

IPC分类号： C12N9/42 ,  C12N15/62 ,  C12N15/56 ,  C12N15/63 ,  D06M16/00 ,  C11D3/386 ,  A23K1/165

CPC分类号： C12Y302/01004 ,  A23K10/14 ,  C07K2319/20 ,  C11D3/38645 ,  C12N9/2437 ,  D06M16/003 ,  D06P5/02 ,  D21C5/005 ,  D21H17/005

摘要： Cellulase fusion proteins comprising an endoglucanase core region and a heterologous cellulose binding domain are described. The fusion proteins may be produced by recombinant techniques using appropriate polynucleotides, expressing vectors and host cells. The fusion proteins and enzyme preparations thereof are useful in treating cellulosic material, such as textile material, and they are particularly useful in biostoning denim or in biofinishing fabrics and garments. In addition the fusion proteins may be used in pulp and paper industry, oil extraction from plants, detergent compositions, or for improving the quality of animal feed.