PHYTASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM
    61.
    发明公开
    PHYTASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM 有权
    肌醇六磷酸酶,用于生产和使用的核酸编码AND METHOD

    公开(公告)号:EP2432797A2

    公开(公告)日:2012-03-28

    申请号:EP10778424.1

    申请日:2010-05-20

    IPC分类号: C07H21/04

    摘要: This invention relates to phytases, polynucleotides encoding them, uses of the polynucleotides and polypeptides of the invention, as well as the production and isolation of such polynucleotides and polypeptides. In particular, the invention provides polypeptides having phytase activity under high temperature conditions, and phytases that retain activity after exposure to high temperatures. The invention further provides phytases which have increased gastric lability. The phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be formulated as foods or feeds or supplements for either to, e.g., aid in the digestion of phytate. The foods or feeds of the invention can be in the form of pellets, liquids, powders and the like. In one aspect, phytases of the invention are stabile against thermal denaturation during pelleting; and this decreases the cost of the phytase product while maintaining in vivo efficacy and detection of activity in feed.

    摘要翻译: 本发明涉及肌醇六磷酸酶,编码它们的多核苷酸,使用该多核苷酸和本发明的多肽,以及寻求多核苷酸和多肽的生产和分离的。 特别地,本发明提供具有多肽植酸酶活性高的温度条件下,和肌醇六磷酸酶没有暴露于高温后保持活性。 本发明提供这些都增加了胃的不稳定性进一步植酸酶。 本发明的肌醇六磷酸酶可以在食品中使用,以提高富植酸盐成分的供给值。 本发明的肌醇六磷酸酶可被配制为食品或饲料或补充剂要么到,E. G.,有助于植酸盐的消化。 本发明的食品或饲料可以是丸剂,液体,粉末等的形式。 在一个方面,本发明的肌醇六磷酸酶是反对造粒过程中的热变性稳定; 这降低了肌醇六磷酸酶的产物,而在体内效力和在进料活性的检测维护成本。

    Arabinofuranosidase enzymes, nucleic acids encoding them and methods for making and using them
    62.
    发明公开
    Arabinofuranosidase enzymes, nucleic acids encoding them and methods for making and using them 有权
    阿拉伯呋喃糖苷酶的酶的核酸,编码这些,以及它们的制备和使用过程

    公开(公告)号:EP2420570A1

    公开(公告)日:2012-02-22

    申请号:EP11007477.0

    申请日:2006-12-08

    IPC分类号: C12N9/42

    摘要: The invention provides polypeptides having any cellulolytic activity, e.g., a cellulase activity, a endoglucanase, a cellobiohydrolase, a beta-glucosidase, a xylanase, a mannanse, a β-xylosidase, an arabinofuranosidase, and/or an oligomerase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having any cellulolytic activity, e.g., a cellulase activity, e.g., endoglucanase, cellobiohydrolase, beta-glucosidase, xylanase, mannanse, β-xylosidase, arabinofuranosidase, and/or oligomerase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. In one aspect, the invention provides polypeptides having an oligomerase activity, e.g., enzymes that convert recalcitrant soluble oligomers to fermentable sugars in the saccharification of biomass. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural, food and feed processing and industrial contexts. The invention also provides compositions or products of manufacture comprising mixtures of enzymes comprising at least one enzyme of this invention.

    摘要翻译: 本发明提供具有任何纤维素分解活性,例如纤维素酶活性,内切葡聚糖酶,纤维二糖水解,β-葡糖苷酶,木聚糖酶,甘露聚糖酶,阿拉伯呋喃糖酶在一个²木糖苷多肽,和/或寡聚体酶活性,多核苷酸编码本文 多肽,以及制备和使用这些多核苷酸和多肽的方法。 在一个方面,本发明涉及具有任何纤维素分解活性,例如纤维素酶活性,例如内切葡聚糖酶,纤维二糖水解,β-葡糖苷酶,木聚糖酶,甘露聚糖酶,²木糖苷酶,阿拉伯呋喃糖苷酶和/或寡聚体酶活性,包括热电表多肽和 耐热活性,多核苷酸编码合成的酶,以及制备和使用这些多核苷酸和多肽。 在一个方面,本发明提供具有对寡聚体酶活性的多肽,E. G.,酶没有转换顽抗可溶性寡聚体可发酵的糖在生物质的糖化。 本发明的多肽可以在多种药物,农业,食品和饲料加工和工业环境中使用。 因此本发明提供的组合物或包含酶包含本发明的至少一种酶的混合物制造的产品。