公开(公告)号：EP4390368A1

公开(公告)日：2024-06-26

申请号：EP22383262.7

申请日：2022-12-22

申请人： European Molecular Biology Laboratory ,  Fundació Centre de Regulació Genòmica

发明人： Cotterell, James ,  Sharpe, James ,  Swoger, James ,  Robert-Moreno, Alexandre

IPC分类号： G01N15/14 ,  G06T7/73 ,  G01N15/10

CPC分类号： G01N15/1425 ,  G01N15/1429 ,  G01N15/1434 ,  G01N2015/144720130101 ,  G01N2015/100620130101 ,  G06T7/73 ,  G06T2207/1005620130101 ,  G06T2207/1006420130101 ,  G06T2207/3002420130101 ,  G01N15/1433

摘要： A method for determining spatial location of one or more biological components (12) in a biological sample (10) is proposed. The method comprises optically encoding (S100) the biological components (12) in the biological sample (10) with a plurality of fluorophores (31, 32, 33, 34) to create a combination of active fluorophore concentrations indicative of the spatial location of the one or more biological components (12); dissociating (S200) the biological components (12) from the biological sample (10) to create dissociated biological components (12) of the biological components (12); and - optically decoding (S300) by measuring the fluorescence (S310) of the dissociated biological components (12) and mapping (S320) to the spatial location of the biological components (12) in the non-dissociated sample (10).

公开(公告)号：EP4332546A3

公开(公告)日：2024-06-05

申请号：EP24151296.1

申请日：2014-03-13

申请人： Cytonome/ST, LLC

发明人： MORAD, Blair D. ,  SHARPE, Johnathan Charles ,  SEDOGLAVICH, Nemanya ,  SHAPIRO, Vladimir ,  EHRLICH, Stuart R. ,  PERRAULT, Donald Francis Jr.

IPC分类号： G01N15/14 ,  G01N15/1404 ,  G01N15/1434 ,  G01N15/10

CPC分类号： G01N2015/145220130101 ,  G01N15/1425 ,  G01N2015/100620130101 ,  G01N2015/140620130101 ,  G01N15/1404 ,  G01N15/1484 ,  G01N15/149

摘要： The present disclosure provides improved particle processing (e.g., cytometry and/or cell purification) systems and methods that can operate in an autonomous fashion. More particularly, the present disclosure provides for assemblies, systems and methods for analyzing, sorting, and/or processing (e.g., purifying, measuring, isolating, detecting and/or enriching) particles (e.g., cells, microscopic particles, etc.) where human intervention is not required and/or is minimized. The systems, assemblies and methods of the present disclosure advantageously improve run performance of particle processing systems (e.g., cell purification systems, cytometers) by significantly reducing and/or substantially eliminating the burden of operation for human intervention by automating numerous functions, features and/or steps of the disclosed systems and methods.

公开(公告)号：EP4361591A1

公开(公告)日：2024-05-01

申请号：EP23168000.0

申请日：2023-04-14

申请人： Cytoaurora Biotechnologies, Inc.

发明人： HUANG, Chung-Er ,  CHEN, Sheng-Wen ,  HO, Hsin-Cheng ,  YE, Guang-Ci

IPC分类号： G01N1/30 ,  G01N15/14 ,  G01N1/31 ,  B01L3/02 ,  B01L3/00 ,  G01N15/00 ,  G01N15/10

CPC分类号： G01N1/30 ,  G01N2015/001920130101 ,  G01N2015/100620130101 ,  G01N2015/141520130101 ,  G01N2015/14220130101 ,  G01N15/1404 ,  G01N2015/148120130101 ,  G01N15/1459 ,  G01N15/1425 ,  G01N15/1484 ,  G01N1/312 ,  B01L3/0268 ,  B01L3/5088 ,  B01L2400/08620130101 ,  G01N2015/01220240101 ,  G01N15/1433 ,  G01N15/1492

摘要： A biological particle analysis method (S100) is provided and includes the following steps: fluorescence staining a liquid specimen (200) through a fluorescence staining process so as to enable a target biological particle (201a) in the liquid specimen (200) to becomes a fluorescence; accommodating the liquid specimen (200) into a pico-droplet generator (21) and using a camera device (23) to take a real-time image of the liquid specimen (200); using the pico-droplet generator (21) to output a target pico-droplet (202a) having the target biological particle (201a) onto a biochip (22) according to the real-time image; removing the fluorescent color of the target biological particle (201a) in the target pico-droplet (202a) through a washing process; and fluorescence staining the target biological particle (201a) captured by the biochip (22) at multiple times through the fluorescence staining process and the washing process, so as to obtain a plurality of fluorescence images respectively corresponding to multiple kinds of biological characterization expressions.

公开(公告)号：EP3382372A1

公开(公告)日：2018-10-03

申请号：EP17162986.8

申请日：2017-03-27

申请人： Koninklijke Philips N.V.

发明人： Paul, VAN DER SLUIS

IPC分类号： G01N15/14 ,  G01N15/02 ,  G01N1/22

CPC分类号： G01N15/1425 ,  G01N15/1429 ,  G01N15/1434 ,  G01N15/1459 ,  G01N2001/2223 ,  G01N2015/0238

摘要： The invention provides an optical particle sensor which uses light sources of different first and second wavelengths. A first light source is used to detect light scattering and also to determine when a particle reaches a target positional area. In response to the particle being determined to reach the target positional area, a second light source is operated to provide a pulse of light, and light emitted from the particle in response to the pulse of light is detected by the same detector.

公开(公告)号：EP2056090B1

公开(公告)日：2018-08-29

申请号：EP08167937.5

申请日：2008-10-30

申请人： Sony Corporation

发明人： Furuki, Motohiro ,  Imanishi, Shingo ,  Shinoda, Masataka ,  Suzuki, Akitoshi ,  Miyake, Kazushi

IPC分类号： G01N15/14 ,  G01N21/64

CPC分类号： G01N15/1425 ,  G01N15/1436 ,  G01N15/1459 ,  G01N15/1484 ,  G01N21/6428 ,  G01N21/645 ,  G01N2015/1006 ,  G01N2015/1497 ,  G01N2021/058 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2201/10 ,  G01N2201/11

摘要： A fine particle measuring method of performing optical measurement of fine particles introduced into a plurality of sample fluidic channels (111) provided at predetermined distances on a substrate (11) by scanning light to the sample fluidic channels (111) is disclosed. The method includes: sequentially irradiating the light to at least two or more reference regions provided together with the sample fluidic channels (111); detecting a change of optical property occurring in the light due to the reference regions; and controlling timing of emission of the light to the sample fluidic channels (111).

公开(公告)号：EP2771669B1

公开(公告)日：2018-08-08

申请号：EP12799610.6

申请日：2012-10-29

申请人： Menarini Silicon Biosystems S.p.A.

发明人： MEDORO, Gianni ,  CALANCA, Alex ,  MANARESI, Nicolò

IPC分类号： G01N21/03 ,  B01L3/00 ,  B01L7/00 ,  G01N15/14

CPC分类号： G01N15/1425 ,  B01L3/502715 ,  B01L3/502761 ,  B01L7/00 ,  B01L2200/0668 ,  B01L2300/046 ,  B01L2300/0645 ,  B01L2300/10 ,  B01L2300/1827 ,  B01L2300/1894 ,  G01N15/1456 ,  G01N15/1484

摘要： Method and device (1b) for performing the optical analysis of particles (2) contained in suspension in a fluid (3) arranged inside a microfluidic device (4) which maintains it at a temperature significantly lower than the ambient temperature; the formation of humidity on the outer surface (8) of the cover of the microfluidic device is avoided by applying a thermal flow (F) which determines an increase in the temperature of the outer surface (8) of the cover to above the condensation temperature (Td), or a reduction in the ambient temperature (and/or humidity) in the vicinity of the cover (8), so as to bring the condensation temperature (Td) (dew point) to below the temperature of the surface (8) of the cover determined by the internal operating temperature.

公开(公告)号：EP3164692A1

公开(公告)日：2017-05-10

申请号：EP15734644.6

申请日：2015-07-01

申请人： Octrolix BV

发明人： SCHREUDER, Frederik ,  HOEKMAN, Marcel ,  DEKKER, Ronal ,  HOSSEINI, Seyed Naser

IPC分类号： G01N15/14 ,  G01N21/05 ,  G01N21/03

CPC分类号： G01N15/1484 ,  B01L2300/0654 ,  G01N15/1425 ,  G01N15/1436 ,  G01N15/1459 ,  G01N21/05 ,  G01N33/483 ,  G01N2015/0065 ,  G01N2015/145 ,  G01N2015/1454 ,  G01N2021/0346 ,  G01N2021/4742 ,  G01N2021/6484 ,  G01N2201/08 ,  G01S17/88

摘要： A flow cytometry system having a flow channel defined through the thickness of a substrate is disclosed. Fluid flowing through the flow channel is illuminated by a first plurality of surface waveguides that are arranged around the flow channel in a first plane, while a second plurality of surface waveguides arranged around the flow channel in a second plane receive light after it has interacted with the fluid. The illumination pattern provided to the fluid is controlled by controlling the phase of the light in the first plurality of surface waveguides. As a result, the fluid is illuminated with light that is uniform and has a low coefficient of variation, improving the ability to distinguish and quantify characteristics of the fluid, such as cell count, DNA content, and the like.

摘要翻译： 公开了具有通过衬底厚度限定的流动通道的流式细胞术系统。 流过流动通道的流体由第一多个表面波导来照明，该第一多个表面波导在第一平面中围绕流动通道布置，而围绕第二平面布置在流动通道周围的第二多个表面波导在与第一平面相互作用之后接收光 流体。 提供给流体的照射图案通过控制第一多个表面波导中的光的相位来控制。 结果，流体被均匀且具有低变异系数的光照射，提高了区分和量化流体特性（例如细胞计数，DNA含量等）的能力。

公开(公告)号：EP3035030A4

公开(公告)日：2017-03-22

申请号：EP14853270

申请日：2014-09-10

申请人： SONY CORP

发明人： MURAKI YOSUKE ,  OTSUKA FUMITAKA ,  KATO YASUNOBU

IPC分类号： G01N15/14 ,  G01N15/10

CPC分类号： G01N15/1404 ,  B01L3/0268 ,  B01L3/502723 ,  B01L3/502776 ,  B01L2200/0652 ,  B01L2400/0439 ,  G01N15/1425 ,  G01N15/1436 ,  G01N15/1459 ,  G01N15/1484 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1081 ,  G01N2015/142 ,  G01N2015/149

摘要： [Object] To provide a particle sorting apparatus, a particle sorting method, and a program that are capable of efficiently sorting particles even when sizes of particles to be sorted are not uniform. [Solving Means] A particle sorting apparatus includes a charge section that imparts charges to at least a part of droplets discharged from an orifice from which a fluid stream is generated, and a charge control section that adjusts a charge amount to be imparted to the droplets by the charge section. Further, the charge control section is structured to adjust the charge amount according to sizes of particles included in the droplets.

摘要翻译： 本发明的目的在于提供一种即使在分选的粒子的大小不均匀的情况下也能够高效率地分选粒子的粒子分选装置，粒子分选方法以及程序。 [解决方案]一种颗粒分拣设备包括：充电部分，该充电部分对从产生流体流的孔口排出的液滴的至少一部分施加电荷;以及充电控制部分，其调整将被赋予液滴的电荷量 由收费部门负责。 此外，充电控制部分被构造成根据包含在液滴中的粒子的尺寸来调节电荷量。

公开(公告)号：EP2717035B1

公开(公告)日：2016-12-07

申请号：EP13004627.9

申请日：2013-09-24

申请人： Palas GmbH Partikel-und Lasermesstechnik

发明人： Weiß, Maximilian ,  Mölter, Leander

IPC分类号： G01N21/27 ,  G01N21/53 ,  G01N15/02 ,  G01N15/14

CPC分类号： G01J3/18 ,  G01N15/1425 ,  G01N15/1459 ,  G01N21/274 ,  G01N21/53 ,  G01N2015/0046

公开(公告)号：EP2950079A4

公开(公告)日：2016-10-26

申请号：EP13872550

申请日：2013-11-19

申请人： SONY CORP

发明人： OTSUKA FUMITAKA ,  MURAKI YOSUKE

IPC分类号： G01N15/14

CPC分类号： G01N15/1425 ,  B07C5/3422 ,  G01N15/1427 ,  G01N15/1459 ,  G01N15/1463 ,  G01N21/27 ,  G01N2015/1006 ,  G01N2015/1406 ,  G01N2015/1481 ,  G01N2015/149

摘要： Provided are a microparticle sorting device, and a method and a program for sorting microparticles capable of stabilizing sorting performance over a prolonged period of time. The microparticle sorting device includes an imaging element and a controller. The imaging element obtains an image of fluid and fluid droplets at a position where the fluid discharged from an orifice which generates a fluid stream is converted into the fluid droplets. The controller controls driving voltage of an oscillation element which gives oscillation to the orifice and/or controls a position of the imaging element based on a state of the fluid in the image and/or a state of a satellite fluid droplet. The satellite fluid droplet does not include microparticles and exists between the position, where the fluid is converted into the fluid droplets, and a fluid droplet, among fluid droplets including the microparticles, which is closest to the position where the fluid is converted into the fluid droplets.