公开(公告)号：EP1715060A4

公开(公告)日：2009-07-22

申请号：EP05704386

申请日：2005-01-28

申请人： KYOWA MEDEX CO LTD

发明人： MIYAUCHI KAZUHITO

IPC分类号： C12Q1/60 ,  C12Q1/26 ,  C12Q1/28 ,  C12Q1/32 ,  C12Q1/34 ,  C12Q1/44 ,  G01N33/92

CPC分类号： C12Q1/60 ,  C12Q1/34 ,  G01N33/92 ,  G01N2800/044

摘要： Ester-form and free-form cholesterols contained in a very-low-density lipoprotein remnant (hereinafter, inclusively referred to as very-low-density lipoprotein remnant cholesterols) are determined. In an aqueous medium containing a sample, a cholesterol ester hydrolase and a cholesterol oxidase or cholesterol dehydrogenase are caused to act specifically on the very-low-density lipoprotein remnant cholesterols in the sample in the presence of a combination of (a) a polyoxyethylene-polyoxyalkylene alkylaryl ether and (b) at least one surfactant selected from the group consisting of a polyoxyethylene/polyoxybutylene condensate, a polyoxyethylene styrenated phenyl ether, and a polyoxyalkylene long-chain alkyl ether.

公开(公告)号：EP1963366A4

公开(公告)日：2009-05-06

申请号：EP06848526

申请日：2006-12-06

申请人： PROMEGA CORP

发明人： GOUELI SAID A ,  HSAIO KUEI-HSUAN ,  KUMAR MEERA ,  VIDUGIRIENE JOLANTA

IPC分类号： C12Q1/00 ,  C07K16/00 ,  C12P21/08 ,  C12Q1/44 ,  C12Q1/527 ,  G01N33/74

CPC分类号： C12Q1/485 ,  C12Q1/008 ,  C12Q1/44 ,  C12Q1/527 ,  C12Q1/66 ,  G01N33/74 ,  G01N2333/16 ,  G01N2333/726 ,  G01N2333/916 ,  G01N2333/988

摘要： The present invention relates in general to cellular analysis tools and more particularly to methods of determining cyclic nucleotide concentrations in samples.

公开(公告)号：EP1307538B1

公开(公告)日：2009-01-07

申请号：EP01963833.7

申请日：2001-08-07

申请人： VANDERBILT UNIVERSITY

发明人： MARNETT, Lawrence, J. ,  KOZAK, Kevin, R.

IPC分类号： C12Q1/26 ,  C12Q1/44 ,  C12Q1/00 ,  G01N33/53

CPC分类号： C12Q1/26 ,  G01N33/573 ,  G01N33/574 ,  G01N33/57484 ,  G01N33/57488 ,  G01N33/58 ,  G01N33/582 ,  G01N33/88

摘要： The present invention relates generally to cyclooxygenase enzymes (COX) and more particularly the COX-2 enzyme. The present invention provides compositions, methods and articles of manufacture (kits) for determining the COX-2 activity in a sample of a subject, such as a patient or a cell culture. The present invention is useful in monitoring inflammation and cancer, or other disease processes, in patients in a clinical setting.

公开(公告)号：EP1807512A4

公开(公告)日：2008-12-31

申请号：EP05732316

申请日：2005-04-06

申请人： STRATAGENE CALIFORNIA

发明人： AREZI BAHRAM

IPC分类号： C07K14/00 ,  C07H21/04 ,  C12N1/21 ,  C12N5/10 ,  C12N9/12 ,  C12N9/16 ,  C12N9/22 ,  C12N15/00 ,  C12P21/06 ,  C12Q1/44 ,  C12Q1/48 ,  C12Q1/68

CPC分类号： C12N9/1276

公开(公告)号：EP1980616A2

公开(公告)日：2008-10-15

申请号：EP08013245.9

申请日：2005-02-25

申请人： Wisconsin Alumni Research Foundation

发明人： Welch, Rodney A. ,  Lathem, Wyndham W. ,  Gyrs, Thomas E. ,  Walters, Laura

IPC分类号： C12N15/31 ,  C12N15/63 ,  C07K14/245 ,  C07K16/12 ,  A61K39/108 ,  C12Q1/44 ,  C12Q1/68 ,  C12N9/52

CPC分类号： C07K16/1232 ,  A61K39/00 ,  A61K2039/505 ,  C07K14/245 ,  C12N9/52 ,  C12Q1/37 ,  C12Q1/689 ,  G01N33/573

摘要： Disclosed is a p0157 plasmid-specified polypeptide found in E.coli EDL933 and other E. coli that binds to and cleaves C1-esterase inhibitor, and antibodies specific for the polypeptide. Also disclosed are methods employing the polypeptide for diagnosing enterohemorragic E. coli infection, identifying potential inhibitors of its acticity, and reducing viscosity of material containing glycosylated polypeptides.

摘要翻译： 公开了在大肠杆菌EDL933和其它大肠杆菌中发现的p0157质粒指定的多肽，其结合并切割C1-酯酶抑制剂，以及对该多肽具有特异性的抗体。 还公开了使用该多肽诊断肠道大肠杆菌感染的方法，鉴定其活性的潜在抑制剂以及降低含有糖基化多肽的物质的粘度的方法。

公开(公告)号：EP1818412A4

公开(公告)日：2008-06-04

申请号：EP05809660

申请日：2005-11-28

申请人： JIMRO CO LTD

发明人： NAKANO TAKAMITU ,  NIIMI MANABU ,  OGIHARA FUMIKO

IPC分类号： C12Q1/26 ,  C12Q1/32 ,  C12Q1/44 ,  G01N33/48 ,  G01N33/92

CPC分类号： C12Q1/44 ,  C12Q1/26 ,  C12Q1/32 ,  G01N33/92

公开(公告)号：EP1647599B1

公开(公告)日：2008-04-30

申请号：EP04742071.6

申请日：2004-07-21

申请人： UNIVERSIDADE DE SANTIAGO DE COMPOSTELA

发明人： Botana Lopez, L.M., Univ. Santiago de Compostela ,  Alfonso Rancano, A., Univ. Santiago de Compostela ,  Pazos Guldris, M.J., Univ. Santiago de Compostela ,  Rodrigeuz Vieytes, M., Univ. Sant. de Compostela ,  Loza Garcia, M.I., Univ. Santiago de Compstela ,  Vieites Baptista De Sousa, Juan Manuel

IPC分类号： C12Q1/44 ,  G01N21/64 ,  A61K31/35 ,  A61P11/06 ,  A61P37/08 ,  A61P35/00

CPC分类号： C12Q1/44 ,  A61K31/35

摘要： The invention relates to a quantitative method for detecting yessotoxins in fish products based on the activation caused by the toxin in cellular phosphodiesterase and therapeutic usefulness of said activation. The cellular target of yessotoxin (YTX) and analogues is the activation of phosphodiesterases (PDEs). The PDEs-YTX union emits a measurable signal. The union can be quantified by means of an affinity biosensor or fluorescence. The biosensor detects bimolecular interactions and makes it possible to determine the presence of YTX due to its interaction with PDEs. Plate fluorescence makes it possible to determine variations in the speed of degradation of fluorescent derivative anthranyloil-AMPc. The speed with which the PDEs degrade this molecule increases in the presence of YTX. The YTX inhibits the immunological activation of rat mastocytese and induces a cytotoxic effect in human hepatocarcinoma cells, which provides two therapeutic utilities of YTXs as anti-allergic and anti-tumoral compound.

公开(公告)号：EP1751541A4

公开(公告)日：2008-03-26

申请号：EP05725500

申请日：2005-03-11

申请人： WINTERLAB LTD

发明人： LIBERMAN BARNET

IPC分类号： G01N33/04 ,  A23L17/00 ,  C12Q1/22 ,  C12Q1/44 ,  G01N1/30 ,  G01N33/12

CPC分类号： G01N33/12 ,  C12Q1/22 ,  C12Q1/44 ,  C12Q2334/00 ,  G01N1/30

摘要： A method of evaluating freshness of a fish product by cutting a small quantity of sample from the fish product, adding an effective amount of a staining reagent containing at least one of a cell-permeant dye and a cell­impermeant fluorescent dye onto the sample, incubating the sample for a predetermined duration, and determining the freshness of the fish product based on the fluorescence emitted from the sample.

公开(公告)号：EP1650200A4

公开(公告)日：2008-03-19

申请号：EP04735395

申请日：2004-05-28

申请人： OSAKA IND PROMOTION ORG

发明人： MAEDA HATSUO ,  ITOH NORIO

IPC分类号： C07D265/38 ,  C07D311/12 ,  C07D311/18 ,  C07D311/80 ,  C07D311/82 ,  C07D493/10 ,  C12Q1/26 ,  C12Q1/44 ,  C12Q1/48 ,  G01N21/64 ,  G01N33/50

CPC分类号： C12Q1/485 ,  C07D265/38 ,  C07D311/12 ,  C07D311/82 ,  C07D493/10 ,  C12Q1/26 ,  C12Q1/44

摘要： A sulfonate compound comprising a structure represented by the following general formula: (I) wherein the atomic group A-O is an atomic group forming a fluorescent compound by cutting the covalent bonding with a sulfonyl group, the atomic group B-SO3- bonding to the atomic group A may be one group or plural groups, B is a ring substituted with one or plural electron-attracting groups, the above electron-attracting group is at least one selected from the group consisting of a halogenated alkyl group, a nitro group and a cyano group, and B’s may be the same or different when a plurality of B are present.

公开(公告)号：EP1718967A4

公开(公告)日：2008-03-05

申请号：EP05712595

申请日：2005-02-03

申请人： DIADEXUS INC

发明人： WOLFERT ROBERT L ,  KIM NAM ,  DUAN XIAOZHU

IPC分类号： G01N33/53 ,  C07D271/08 ,  C07K16/00 ,  C12Q1/44 ,  G01N33/573

CPC分类号： C12Q1/44 ,  C07D271/08 ,  G01N2333/918 ,  G01N2800/32

摘要： This invention relates to a method for measuring enzymatically active Lipoprotein­associated Phospholipase A2 (Lp-PLA2) in a sample. Further, this invention relates to a Hybrid Immunocapture method for measuring enzymatically active Lp-PLA2 in a sample. Specifically, this invention relates to a Hybrid Immunocapture method for measuring enzymatically active Lp-PLA2 in a sample utilizing an enzymatically active Lp-PLA2 standard. In addition, this invention relates to a kit for measuring enzymatically active Lp­PLA2 in a sample. Specifically, this invention relates to a kit for measuring enzymatically active Lp-PLA2 in a sample containing an enzymatically active Lp-PLA2 standard.