公开(公告)号：EP1698345A2

公开(公告)日：2006-09-06

申请号：EP06006483.9

申请日：1999-07-28

申请人： University of Maryland, Baltimore

发明人： Fasano, Alessio

IPC分类号： A61K38/00

CPC分类号： C07K7/06 ,  A61K38/00 ,  C07K7/08 ,  C07K14/4705 ,  C07K14/4716

摘要： The present invention relates to peptide antagonists of zonulin, as well as methods for the use of the same. Said peptide antagonists bind to the zonula occludens receptor, yet do not physiologically modulate the opening of mammalian tight junctions.

摘要翻译： 本发明涉及zonulin的肽拮抗剂，以及使用它们的方法。 所述肽拮抗剂结合于囊膜闭塞受体，但不生理调节哺乳动物紧密连接的开放。

公开(公告)号：EP0972037B1

公开(公告)日：2006-08-16

申请号：EP98912994.5

申请日：1998-03-20

申请人： THE JOHNS HOPKINS UNIVERSITY ,  Kylix B.V.

发明人： BARKER, Nick ,  CLEVERS, Hans ,  KINZLER, Kenneth, W. ,  KORINEK, Vladimir ,  MORIN, Patrice, J. ,  SPARKS, Andrew, B. ,  VOGELSTEIN, Bert

IPC分类号： C12N15/12 ,  C07K14/47 ,  C12Q1/68 ,  G01N33/50 ,  A61K38/17 ,  A61K48/00

CPC分类号： C07K14/47 ,  A61K38/00 ,  A61K48/00 ,  C07K14/4705 ,  C12Q1/6897

摘要： The APC tumor suppressor protein binds to beta -catenin, a protein recently shown to interact with Tcf/Lef transcription factors. Here, the gene encoding a Tcf family member that is expressed in colonic epithelium (hTcf-4) was cloned and characterized. hTcf-4 transactivates transcription only when associated with beta -catenin. Nuclei of APC colon carcinoma cells were found to contain a stable beta -catenin-hTCF-4 complex that was constitutively active, as measured by transcription of a Tcf reporter gene. Reintroduction of APC removed beta -catenin from hTcf4 and abrogated the transcriptional transactivation. Constitutive transcription of TCF target genes, caused by loss of APC function, may be a crucial event in the early transformation of colonic epithelium. It is also shown here that the products of mutant APC genes found in colorectal tumors are defective in regulating beta -catenin/Tcf-4 transcriptional activation. Furthermore, colorectal tumors with intact APC genes were shown to contain subtle activating mutations of beta -catenin that altered functionally significant phosphorylation sites. These results indicate that regulation of beta -catenin is critical to APC's tumor suppressive effect and that this regulation can be circumvented by mutations in either APC or beta -catenin.

公开(公告)号：EP1664769A2

公开(公告)日：2006-06-07

申请号：EP04740371.2

申请日：2004-06-28

申请人： Orion Diagnostica Oy

发明人： VUOLTEENAHO, Olli ,  ALA-KOPSALA, Minna ,  RUSKOAHO, Heikki ,  LEPPÄLUOTO, Juhani ,  HAAPALAHTI, Jouko

IPC分类号： G01N33/50

CPC分类号： G01N33/74 ,  C07K14/47 ,  C07K14/4705 ,  C07K16/18 ,  C07K16/26 ,  C07K2319/00 ,  G01N33/6893 ,  G01N2333/58 ,  G01N2800/325

摘要： An in vitro method of determining activation or inactivation of the atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) hormonal systems, the method comprising simultaneously detecting the presence or amount of atrial and brain natriuretic peptide prohormones (proANP and proBNP) or fragments thereof in a sample.

公开(公告)号：EP1240822B1

公开(公告)日：2006-05-03

申请号：EP00953502.2

申请日：2000-08-18

申请人： Japan Science and Technology Agency

发明人： ISHINO, Fumitoshi ,  MIYOSHI, Naoki ,  ISHINO, Tomoko ,  YOKOYAMA, Minesuke ,  WAKANA, Shigeharu

IPC分类号： A01K67/027 ,  C12N15/12 ,  C12Q1/68 ,  C12Q1/02 ,  A61K45/00 ,  A61P3/10 ,  G01N33/50 ,  G01N33/15

CPC分类号： C12N15/8509 ,  A01K67/0273 ,  A01K67/0275 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2227/105 ,  A01K2267/03 ,  A01K2267/0306 ,  C07K14/4705

摘要： A diabetes onset model mammal which is useful in clarifying the onset mechanism of diabetes induced by the inhibition of signal transduction from insulin and developing remedies for such diabetes; and a method of screening a remedy for such diabetes. A transgenic mouse is prepared by constructing a transgene, which carries an imprinting gene Meg1/Grb10 showing maternal expression or human GRB10 gene in the downstream of chicken β-actin promoter and in the upstream of rabbit β-globin polyA; microinjecting this transgene into the male pronucleus of a mouse fertilized ovum; incubating the thus obtained ovule; transplanting it into the Fallopian tube of a mouse under pseudopregnancy; feeding the thus transplanted animal; and selecting an individual having the above-described cDNA from among the litter of mice.

公开(公告)号：EP1005480B1

公开(公告)日：2006-03-22

申请号：EP98935560.7

申请日：1998-07-07

申请人： University of Massachusetts

发明人： DAVIS, Roger, J. ,  WHITMARSH, Alan ,  TOURNIER, Cathy

IPC分类号： C07K14/07 ,  C07K14/47 ,  A61K38/17

CPC分类号： C07K14/005 ,  A61K38/00 ,  C07K14/4705 ,  C07K14/4736 ,  C07K14/82 ,  C12N9/12 ,  C12N9/1205 ,  C12N2710/10322 ,  C12N2710/10332 ,  C12Q1/485 ,  C12Q1/6883 ,  C12Q2600/158 ,  G01N2500/02

摘要： Disclosed are human mitogen-activated (MAP) kinase kinase isoforms (MKKs). MKKs mediate unique signal transduction pathways that activate human MAP kinases p38 and JNK, which result in activation of other factors, including activating transcription factor-2 (ATF2) and c-Jun. The pathways are activated by a number of factors, including cytokines and environmental stress. Methods are provided for identifying reagents that modulate MKK function or activity and for the use of such reagents in the treatment of MKK-mediated disorders.

公开(公告)号：EP1600510A3

公开(公告)日：2006-02-22

申请号：EP05076209.5

申请日：2002-06-14

申请人： ChromaGenics B.V.

发明人： Otte, Arie Pieter ,  Kruckeberg, Arthur Leo

IPC分类号： C12N15/67

CPC分类号： C12Q1/6897 ,  C07K14/4702 ,  C07K14/4705 ,  C12N15/1034 ,  C12N15/635 ,  C12N15/8216 ,  C12N15/8218

摘要： The invention is concerned with the systematic elucidation and identification of regulatory sequences. The invention provides among others screenings and detection methods with which regulatory sequences can be identified. The invention further provides regulatory sequences and use thereof in various fields such as, but not limited to protein production, diagnostics, transgenic plants and animals, and the therapeutic field.

公开(公告)号：EP1475436A4

公开(公告)日：2005-12-07

申请号：EP03703156

申请日：2003-02-04

申请人： NAT INST OF AGROBIO SCIENCES

发明人： YASUE HIROSHI ,  WATANABE SATOSHI ,  HONMA DAISUKE ,  TAKAGAKI YOHTAROH ,  HATSUSE HIROMI

IPC分类号： A01K67/027 ,  C07K14/47 ,  C07K16/18 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/00 ,  C12N15/09 ,  C12P21/02 ,  A01K67/00 ,  C12N5/16

CPC分类号： C07K14/4705 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2227/108 ,  A01K2267/02

摘要： By screening a swine genomic DNA library, a BAC clone containing a swine RAG-1 gene is obtained. From this BAC clone, RAG-1 gene-containing regions are subcloned to give 3 clones. These clones are sequenced to determine the base sequences and mapped by digesting with restriction enzymes, thereby form gene maps. Based on the thus obtained data, a targeting vector for knockouting the RAG-1 gene is constructed. By regulating the function of the endogenous swine RAG-1 gene with the use of this vector, a swine having no acquired immunity can be constructed.

公开(公告)号：EP1600510A2

公开(公告)日：2005-11-30

申请号：EP05076209.5

申请日：2002-06-14

申请人： ChromaGenics B.V.

发明人： Otte, Arie Pieter ,  Kruckeberg, Arthur Leo

IPC分类号： C12N15/67 ,  C12N15/85

CPC分类号： C12Q1/6897 ,  C07K14/4702 ,  C07K14/4705 ,  C12N15/1034 ,  C12N15/635 ,  C12N15/8216 ,  C12N15/8218

摘要： The invention is concerned with the systematic elucidation and identification of regulatory sequences. The invention provides among others screenings and detection methods with which regulatory sequences can be identified. The invention further provides regulatory sequences and use thereof in various fields such as, but not limited to protein production, diagnostics, transgenic plants and animals, and the therapeutic field.

摘要翻译： 本发明涉及系统阐明和鉴定调控序列。 本发明提供了其中可以鉴定调节序列的筛选和检测方法。 本发明还提供了调节序列及其在各种领域中的应用，例如但不限于蛋白质生产，诊断，转基因植物和动物以及治疗领域。

公开(公告)号：EP1206542B1

公开(公告)日：2005-11-09

申请号：EP00954207.7

申请日：2000-08-17

申请人： Centre for Translational Research in Cancer

发明人： ALAOUI-JAMALI, Moulay, A. ,  CHO, John Myung-Jae

IPC分类号： C12N15/12 ,  C12N15/62 ,  C07K14/47 ,  A61K48/00

CPC分类号： C07K14/4705 ,  A61K48/00 ,  C07K2319/00

摘要： The present invention relates to replication protein A (RPA) transcriptional factors. There is provided a nucleotide sequence encoding a replication protein A transcriptional activator 1 (RBT1) and a protein encoded by such a nucleotide sequence. RBT1 has a high activity and is highly transactivated in cancer cells. The sequence may be used to treat neoplastic disorders and in gene therapy.

公开(公告)号：EP1183355B1

公开(公告)日：2005-11-02

申请号：EP00939546.8

申请日：2000-06-01

申请人： PRESIDENT AND FELLOWS OF HARVARD COLLEGE

发明人： GLIMCHER, Laurie, H. ,  SZABO, Susanne, J.

IPC分类号： C12N15/12 ,  C12N5/10 ,  C07K14/47 ,  C07K16/18 ,  G01N33/50 ,  C12Q1/68 ,  A01K67/027 ,  A61K38/17

CPC分类号： C07K14/4705 ,  A01K2217/05 ,  A61K38/00 ,  C07K2319/00

摘要： Isolated nucleic acid molecules encoding T-bet, and isolated T-bet proteins, are provided. The invention further provides antisense nucleic acid molecules, recombinant expression vectors containing a nucleic acid molecule of the invention, host cells into which the expression vectors have been introduced and non-human transgenic animals carrying a T-bet transgene. The invention further provides T-bet fusion proteins and anti-T-bet antibodies. Methods of using the T-bet compositions of the invention are also disclosed, including methods for detecting T-bet activity in a biological sample, methods of modulating T-bet activity in a cell, and methods for identifying agents that modulate the activity of T-bet.