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    A method of selectively producing male or female sterile plants
    73.
    发明公开
    A method of selectively producing male or female sterile plants 审中-公开
    赫尔斯捷兰德·韦斯特伦

    公开(公告)号：EP2287322A1

    公开(公告)日：2011-02-23

    申请号：EP10178004.7

    申请日：2003-02-14

    申请人： Syngenta Limited

    发明人： Hadfield, Stephen Thomas ,  Hawkes, Timothy Robert ,  Mitchell, Glynn ,  Thompson, Paul Anthony ,  Viner, Russell Colin ,  Zhang, Yan

    IPC分类号： C12N15/82 ,  C12N9/18 ,  C12N9/06 ,  C07F9/38 ,  C12P13/04 ,  C12P17/12

    CPC分类号： C12N9/0024 ,  C12N9/18 ,  C12N15/8287 ,  C12N15/8289 ,  C12N15/829 ,  C12P13/04 ,  C12P41/007

    摘要： A method of producing male or female sterile plants comprising the steps of transforming plant material with a polynucleotide which encodes at least one enzyme which reacts with a non-phytotoxic substance to produce a phytotoxic one, and regenerating the thus transformed material into a plant, wherein the said non-phytotoxic substance is applied to the plant up to the time of male or female gamete formation and/ or maturation, so that the non-phytotoxic substance provides for the production of a phytotoxic one which selectively prevents the formation of or otherwise renders the said gametes non-functional, wherein the enzyme is expressed preferentially in either male or female reproductive structures, characterised in that (i) the non-phytotoxic substance is selected from the group consisting of ester derivatives of non-phosphonate herbicides which herbicides are directly phytotoxic to non-green tissue, D-alpha amino acids, peptide derivatives of non-protein D-alpha amino acids, S-enantiomers of aryloxyphenoxypropionates and S-enantiomers of ester derivatives of aryloxyphcnoxypropionatcs and (ii) the enzyme is selected from the group consisting of carboxylesterases, D-amino acid oxidases, D-amino acid dehydrogenases, D-amino acid racemases, 2-arylpropionyl-CoA epimerases, alpha-methylacyl-CoA racemases, thioesterases and acyl-CoA synthetases.

    摘要翻译： 一种产生雄性或雌性不育植物的方法，包括以编码至少一种与非植物毒性物质反应产生植物毒性物质的酶的多核苷酸转化植物材料并将由此转化的材料再生成植物的步骤，其中 所述非植物毒性物质直到雄性或雌性配子形成和/或成熟时被施用于植物，使得非植物毒性物质提供了植物毒性物质的生产，其选择性地防止形成或以其他方式形成 所述配子是非功能性的，其中所述酶优先在雄性或雌性生殖结构中表达，其特征在于（i）非植物毒性物质选自除草剂直接的非膦酸酯除草剂的酯衍生物 对非绿色组织的植物毒性，D-α氨基酸，​​非蛋白D-α氨基酸的肽衍生物，S-enan 芳氧基丙氧基丙酸酯的酯衍生物的芳氧基苯氧基丙酸酯和S-对映异构体的一部分和（ii）该酶选自羧酸酯酶，D-氨基酸氧化酶，D-氨基酸脱氢酶，D-氨基酸消旋酶，2-芳基丙酰辅酶A 差向异构酶，α-甲基酰基-CoA消旋酶，硫酯酶和酰基辅酶A合成酶。

    Esterases and related nucleic acids and methods
    74.
    发明公开
    Esterases and related nucleic acids and methods 审中-公开
    酯酶和相关的核酸和方法

    公开(公告)号：EP2216403A3

    公开(公告)日：2010-11-24

    申请号：EP10001560.1

    申请日：2007-02-02

    申请人： Verenium Corporation

    发明人： Mathur, Eric J. ,  Callen, Walter ,  Fielding, Roderick

    IPC分类号： C12N15/00 ,  C12N9/16 ,  C12N9/18 ,  C12N9/20 ,  C12P21/06

    CPC分类号： C12N9/14 ,  Y02E50/16

    摘要： The invention provides hydrolases, polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides, e.g., enzymes, having a hydrolase activity, e.g., an esterase, acylase, lipase, phospholipase (e.g. phosphplipase A, B, C and D activity, patatin activity, lipid acyl hydrolase (LAH) activity) or protease activity, including thermostable and thermotolerant hydrolase activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The hydrolase activities of the polypeptides and peptides of the invention include esterase activity, lipase activity (hydrolysis of lipids), acidolysis reactions (to replace an esterified fatty acid with a free fatty acid), transesterification reactions (exchange of fatty acids between triglycerides), ester synthesis, ester interchange reactions, phospholipase activity and protease activity (hydrolysis of peptide bonds). The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts, including the manufacture of cosmetics and nutraceuticals. In another aspect, the polypeptides of the invention are used to synthesize enantiomerically pure chiral products.

    A NOVEL GROUP OF ESTERASES FOR THE PRODUCTION OF FINE AND SPECIALITY CHEMICALS
    79.
    发明公开
    A NOVEL GROUP OF ESTERASES FOR THE PRODUCTION OF FINE AND SPECIALITY CHEMICALS 有权
    酯酶为精细和专用化学品生产新型GROUP

    公开(公告)号：EP2013336A2

    公开(公告)日：2009-01-14

    申请号：EP07724699.9

    申请日：2007-04-27

    申请人： B.R.A.I.N. AG

    发明人： ELEND, Christian ,  JAEGER, Karl-Erich ,  LEGGEWIE, Christian ,  SCHMEISSER, Christel ,  STREIT, Wolfgang

    IPC分类号： C12N9/18

    CPC分类号： C12N9/18

    摘要： The present invention relates to a polynucleotide encoding an enzyme having carboxyl esterase [E. C. 3.1.1.1] activity, wherein the coding sequence is selected from the group consisting of (a) a polynucleotide encoding an amino acid sequence as depicted in any one of SEQ ID NOs: 2, 4, 6 and 8; (b) a polynucleotide having or comprising a nucleotide sequence encoding an enzyme, wherein the nucleic acid sequence is as shown in any one of SEQ ID NOs: 1, 3, 5 and 7; (c) a polynucleotide having or comprising a nucleotide sequence encoding a fragment or derivative of an enzyme encoded by a polynucleotide of (a) or (b), wherein in said derivative one or more amino acid residues are conservatively substituted compared to the amino acid sequence of (a); (d) a polynucleotide encoding an enzyme having carboxyl esterase activity which polynucleotide is at least 65% identical to a polynucleotide encoding an enzyme as shown in one of SEQ ID NOs: 2, 4, 6 and 8; (e) a polynucleotide having or comprising a nucleotide sequence the complementary strand of which hybridizes to a polynucleotide as defined in any one of (a) to (d); and (T) a polynucleotide having or comprising a nucleotide sequence being degenerate to the nucleotide sequence of the polynucleotide of (d) or (e); or the complementary strand of such a polynucleotide of (a) to (f) or fragments thereof useful as specific probes or primers. The present invention also relates to a host, genetically engineered with the polynucleotide of the present invention or the vector of the present invention. The present invention also relates to a polypeptide comprising the amino acid sequence encoded by a polynucleotide of the present invention or which is obtainable by the process of the present invention. Moreover, the present invention relates to a process for producing said polypeptide and for producing bacteria expressing said polypeptide. Finally, the present invention relates to a composition comprising the polynucleotide of the present invention, the vector of the present invention, the host of the present invention, the polypeptide of the present invention, the antibody of the present invention and/or one or more primers of the present invention.