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    METHOD OF PRODUCING SHORT-CHAIN PEPTIDE IMMOBILIZATION CARRIER AND SHORT-CHAIN PEPTIDE IMMOBILIZATION CARRIER
    71.
    发明公开
    METHOD OF PRODUCING SHORT-CHAIN PEPTIDE IMMOBILIZATION CARRIER AND SHORT-CHAIN PEPTIDE IMMOBILIZATION CARRIER 审中-公开

    公开(公告)号：EP3284757A4

    公开(公告)日：2018-02-21

    申请号：EP16779934

    申请日：2016-04-01

    申请人： FUJIFILM CORP

    发明人： MINAMI KOICHI

    IPC分类号： C07K17/06

    CPC分类号： C07K17/10 ,  C07K17/06

    摘要： Provided is a method for producing a short-chain peptide-immobilized carrier that maintains a secondary structure of a short-chain peptide, the method including a step of preparing an alcohol solution containing an alcohol solvent, and a short-chain peptide having a plurality of immobilizing functional groups, the short-chain peptide having a secondary structure induced in the alcohol solvent; and a step of bringing a carrier coupled with a spacer having a reactive group that reacts with the immobilizing functional group, into contact with the alcohol solution, and thereby immobilizing the short-chain peptide to the spacer.

    PEPTIDE-LIPID CONSTRUCTS AND THEIR USE IN A FC-FUNCTION ASSAY
    75.
    发明公开
    PEPTIDE-LIPID CONSTRUCTS AND THEIR USE IN A FC-FUNCTION ASSAY 审中-公开
    肽 - 脂质构建物及其在FC功能测定中的用途

    公开(公告)号：EP2681559A1

    公开(公告)日：2014-01-08

    申请号：EP12753030.1

    申请日：2012-03-05

    申请人： Kode Biotech Limited

    发明人： HENRY, Stephen Micheal ,  KOMARRAJU, Venkata Sarvani

    IPC分类号： G01N33/555 ,  C07K14/00 ,  C07F9/10 ,  C07K17/06 ,  G01N33/53 ,  G01N33/531 ,  G01N33/536 ,  C07K14/045 ,  G01N33/68 ,  A61K39/385 ,  A61K31/683 ,  A61K38/16

    CPC分类号： G01N33/6854 ,  C07F9/65583 ,  C07K14/005 ,  C12N2710/16122 ,  G01N33/6857 ,  G01N2333/045 ,  G01N2333/4716

    摘要： The present invention discloses a method of assaying Fc-function in an immunoglobulin containing sample by contacting the sample, in the presence of a complement, with red blood cells modified to incorporate a F-S-L peptide- lipid construct. The F-S-L peptide construct comprises a functional group (F) that is a target antigen for a complement activating immunoglobulin, a spacer group (S) covalently linking F to L and a lipid group (L). The present invention further discloses a specific construct, DOPE-Ad-CMG(2)-hCMV2 (see below) where the F group is an antigen of cytomegalovirus, that is used in assaying the Fc function of an intravenous immunoglobulin (IVIG) product.

    摘要翻译： 本发明公开了通过使样品在补体存在下与经修饰以掺入F-S-L肽 - 脂质构建体的红血细胞接触来测定含免疫球蛋白样品中的Fc-功能的方法。 F-S-L肽构建体包含作为补体活化免疫球蛋白的靶抗原的官能团（F），共价连接F至L的间隔基团（S）和脂质基团（L）。 本发明还公开了特异性构建体DOPE-Ad-CMG（2）-hCMV2（见下文），其中F基团是巨细胞病毒的抗原，其用于测定静脉内免疫球蛋白（IVIG）产物的Fc功能。

    Process of production of a recombinant polypeptide by fusion with an autoprotease
    80.
    发明公开
    Process of production of a recombinant polypeptide by fusion with an autoprotease 有权
    生产方法von einem rekombinant多肽纯合酶融合蛋白酶自身蛋白酶。

    公开(公告)号：EP2366719A2

    公开(公告)日：2011-09-21

    申请号：EP11167687.0

    申请日：2006-04-25

    申请人： Sandoz AG ,  Boehringer Ingelheim RCV GmbH & Co KG

    发明人： Werther, Florian ,  Achmüller, Clemens ,  Wechner, Philipp ,  Auer, Bernhard ,  Podmirseg, Silvio

    IPC分类号： C07K17/06 ,  C07K1/22 ,  C12N15/62 ,  C12P21/02 ,  C12N9/50 ,  C12P21/06

    CPC分类号： C12P21/02 ,  C07K1/22 ,  C07K17/06 ,  C12N9/506 ,  C12N15/62

    摘要： The invention relates to a process for the recombinant production of a heterologous polypeptide of interest, comprising,
    (i) cultivation of a bacterial host cell which is transformed with an expression vector which comprises a nucleic acid molecule which codes for a fusion polypeptide, the fusion polypeptide comprising a derivative of an autoprotease N pro of Pestivirus, wherein at least one cysteine residue of the naturally occuring autoprotease N pro of Pestivirus is replaced by another amino acid residue, and a second polypeptide which is connected to the first polypeptide at the C-terminus of the first polypeptide in a manner such, that the second polypeptide is capable of being cleaved from the fusion polypeptide by the autoproteolytic activity of the first polypeptide, said second polypeptide being a heterologous polypeptide, wherein cultivation occurs under conditions which cause expression of the fusion polypeptide and formation of corresponding cytoplasmic inclusion bodies,
    (ii) isolation of the inclusion bodies from the host cell,
    (iii) solubilization of the isolated inclusion bodies,
    (iv) induction of autoproteolytic cleavage of the heterologous polypeptide of interest from the fusion polypeptide, and
    (v) isolation of the cleaved heterologous polypeptide of interest.

    摘要翻译： 本发明涉及重组产生感兴趣的异源多肽的方法，其包括（i）培养用表达载体转化的细菌宿主细胞，所述表达载体包含编码融合多肽的核酸分子，所述融合 其包含天蛾病毒自身蛋白酶N pro的至少一个半胱氨酸残基被另一个氨基酸残基替代，第二个多肽与第一多肽在C-末端相连， 第一多肽的第一个多肽的末端以第二多肽能够通过第一多肽的自身蛋白水解活性从融合多肽切割，所述第二多肽是异源多肽，其中培养发生在引起表达的条件下 融合多肽和形成相应的细胞质包涵体，（i i）从宿主细胞中分离包涵体，（iii）分离的包涵体的溶解，（iv）从融合多肽诱导感兴趣的异源多肽的自体蛋白水解切割，和（v）分离的切割的异源多肽 出于兴趣。