公开(公告)号：EP0420470A3

公开(公告)日：1991-07-10

申请号：EP90310172.3

申请日：1990-09-18

申请人： KYOWA HAKKO KOGYO KABUSHIKI KAISHA

发明人： Toki, Shinichiro ,  Nozawa, Mika ,  Yoshida, Mayumi ,  Sano, Hiroshi ,  Ando, Katsuhiko ,  Kawamoto, Isao ,  Matsuda, Yuzuru ,  Ikeda, Junichi ,  Kubo, Kazuhiro

IPC分类号： C07D311/92 ,  A61K31/35 ,  C12P17/16

CPC分类号： C12P17/162 ,  C07D311/92

摘要： A compound of formula (I)
wherein R₁ represents hydrogen, hydroxy or acetoxy and R₂ represents hydroxy or acetoxy. The compounds are capable of protecting the nerve cells and also antagonistic activity against N-methyl-D-­aspartic acid receptors. They may be prepared by fermentation of a microorganism of the genus Verticillium or a mutant thereof.

公开(公告)号：EP0390592A2

公开(公告)日：1990-10-03

申请号：EP90303445.2

申请日：1990-03-30

申请人： KYOWA HAKKO KOGYO KABUSHIKI KAISHA ,  CENTRAL INSTITUTE FOR EXPERIMENTAL ANIMALS

发明人： Sekine, Susumu ,  Ito, Seiga ,  Katsuki, Motoya

IPC分类号： C12N15/00 ,  C12N15/62 ,  C12N15/58 ,  C12N15/90

CPC分类号： C12N15/8509 ,  A01K67/0278 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2227/10 ,  A01K2227/105 ,  A01K2267/01 ,  C07K14/4732 ,  C07K14/595 ,  C07K2319/00 ,  C07K2319/02 ,  C12N9/6462 ,  C12N15/70 ,  C12N15/85 ,  C12Y304/21073

摘要： A recombinant vector suitable for industrially making an animal produce useful protein in the milk, which is constructed by extracting high molecular weight DNA from the bovine liver to prepare a chromosomal gene library, preparing a synthetic DNA probe corresponding to the publicly known bovine αS1 casein chromosomal gene, screening a clone carrying bovine αS1 casein chromosomal gene, confirming that the clone has a base sequence coincident with that of the publicly known bovine αS1 casein chromosomal gene, ligating human prourokinase cDNA with the promoter part and the intron 1 part of the gene, and then inserting the obtained plasmid into a suitable vector. A transgenic mouse prepared by using this recombinant vector secreted a considerable amount of human prourokinase as useful protein in its milk after delivery.

摘要翻译： 适用于工业制造动物的重组载体通过从牛肝中提取高分子量DNA以制备染色体基因文库，制备对应于公知牛αS1酪蛋白的合成DNA探针而在乳中产生有用的蛋白质 染色体基因，筛选携带牛αS1酪蛋白染色体基因的克隆，证实该克隆具有与公知的牛α1S酪蛋白染色体基因一致的碱基序列，将人类prourokinase cDNA与启动子部分和内含子1部分连接 该基因，然后将获得的质粒插入合适的载体中。 通过使用该重组载体制备的转基因小鼠在分娩后在其牛奶中分泌大量的人类尿激酶作为有用的蛋白质。

公开(公告)号：EP0156578B1

公开(公告)日：1990-03-07

申请号：EP85301678.0

申请日：1985-03-11

申请人： KYOWA HAKKO KOGYO KABUSHIKI KAISHA

发明人： Yoshida, Hajime ,  Hanai, Nobuo

IPC分类号： C12N15/00 ,  C12N5/00 ,  C12P21/00 ,  G01N33/577 ,  A61K39/395

CPC分类号： C07K16/3023 ,  C07K16/3046 ,  Y10S424/807 ,  Y10S530/806 ,  Y10S530/808 ,  Y10S530/828 ,  Y10S530/864

公开(公告)号：EP0336713A3

公开(公告)日：1990-01-24

申请号：EP89303321.7

申请日：1989-04-04

申请人： KYOWA HAKKO KOGYO KABUSHIKI KAISHA

发明人： Itoh, Kunio ,  Sugiyama, Kiyoshi ,  Ohta, Motohiro

IPC分类号： A61K9/52 ,  A23K1/18

CPC分类号： A61K9/5047 ,  A23K40/35 ,  A61K9/5015 ,  A61K9/5026 ,  Y10S426/807

摘要： A coating agent for delaying the release of a physiologically active substance to be administered per os to ruminants, comprising a veterinarially acceptable water-soluble, synthetic high molecular compound and ethylcellulose, said coating agent being stable in the first stomach of ruminants and capable of being effectively disintegrated in the fourth stomach of ruminants, characterized in that said coating agent further comprises at least one substance which is miscible with both of said high molecular weight compound and ethylcellulose and is insoluble in water. The coating agent protects the active substance from degradation in the first stomach of a ruminant at a pH of about 5.5 and rapidly breaks down in the fourth stomach at a pH of about 3, so releasing the active ingredient for absorption by the animal.

公开(公告)号：EP0272064A3

公开(公告)日：1989-06-07

申请号：EP87310938.3

申请日：1987-12-11

申请人： KYOWA HAKKO KOGYO KABUSHIKI KAISHA

发明人： Fujio, Tatsuro ,  Maruyama, Akihiko

IPC分类号： C12P17/04

CPC分类号： C12P17/04

摘要： The invention relates to a process for the preparation of ascorbic acid-2-phosphate, which comprises reacting ascorbic acid or araboascorbic acid with ATP to produce ascorbic acid-2-phosphate in an aqueous medium in the presence of an effective amount of an enzyme derived from a microorganism and capable of catalyzing the enzymatic reaction of ascorbic acid or araboascorbic acid with ATP to produce ascorbic acid-2-phosphate and recovering the resultant ascorbic acid-2-phosphate from the reaction solution.

公开(公告)号：EP0242160A3

公开(公告)日：1989-05-10

申请号：EP87303208.0

申请日：1987-04-13

申请人： KYOWA HAKKO KOGYO KABUSHIKI KAISHA

发明人： Tokuyuki, Kuroda ,  Koji, Hisamura ,  Tohru, Sugaya ,  Hideo, Ueno ,  Tadashi, Ashizawa ,  Yukata, Ohsawa ,  Makoto, Morimoto

IPC分类号： C07D487/14 ,  C07D487/04 ,  A61K31/40

CPC分类号： C07D487/14

摘要： Mitomycin derivatives having potent antitumour activity have the formula:
wherein R represents lower alkyl, cycloalkyl or unsubstituted or substituted aralkyl;
    X represents hydrogen or carbamoyl;
    Y and Z each independently represents hydrogen or methyl;
    and represents an α or β bond.

摘要翻译： 具有有效抗肿瘤活性的丝裂霉素衍生物具有下式：其中R代表低级烷基，环烷基或未取代或取代的芳烷基; X代表氢或氨基甲酰基; Y和Z各自独立地表示氢或甲基; 并表示α或β键。

公开(公告)号：EP0299065A1

公开(公告)日：1989-01-18

申请号：EP86903566.7

申请日：1986-05-23

申请人： KYOWA HAKKO KOGYO KABUSHIKI KAISHA

发明人： MORIMOTO, Kiyoshi ,  MORIYAMA, Masuo

IPC分类号： B01F15/04

CPC分类号： B01F15/0445 ,  B01F3/18 ,  B30B11/08 ,  B30B15/302

摘要： Apparatus for automatic weighing, feeding and mixing of raw granular or powder materials, consisting of storage tanks for at least two different kinds of materials, means for weighing desired quantities of the raw materials from the storage tanks, means for discharging and transferring the weighed granular or powder materials into a mixing tank, a means for mixing the weighed granular or powder materials, and a means for holding a constant quantity of the resultant mixture, all of these means being integrally arranged.

摘要翻译： 自动称粒，供应和混合约会。 包括单元，用于存储和称出至少两种类型的原料颗粒，一混合单元，单位为传递环称出的颗粒的混合单元和用于存储一组AMT一个单元的。混合材料在整体组件中提供了。

公开(公告)号：EP0294762A1

公开(公告)日：1988-12-14

申请号：EP88109054.2

申请日：1988-06-07

申请人： KYOWA HAKKO KOGYO KABUSHIKI KAISHA

发明人： Saito, Yutaka ,  Kasai, Masaji

IPC分类号： C07C103/19 ,  C07C102/00

CPC分类号： C07C237/26

摘要： This invention provides a new process for producing 7-amino-6-demethyl-6-deoxytetracycline (Compound 3) which is an intermediate for the synthesis of minocycline. Compound 3 can be obtained by reduction of Compound 1 using a dithionite as reducing agent. This invention also provides a method to produce Compound 3 from Compound 2 in one pot.
(wherein Ar denotes a substituted aryl group, and X stands for the residue of a mineral acid from which hydrogen atom has been eliminated).

摘要翻译： 本发明提供了用于合成米诺环素的中间体7-氨基-6-脱甲基-6-脱氧四环素（化合物3）的新方法。 可以使用连二亚硫酸盐还原剂还原化合物1来获得化合物3。 本发明还提供了在一锅中从化合物2制备化合物3的方法。 （其中Ar表示取代的芳基，X表示除去氢原子的无机酸的残基）。

公开(公告)号：EP0289616A1

公开(公告)日：1988-11-09

申请号：EP87906789.0

申请日：1987-10-19

申请人： KYOWA HAKKO KOGYO KABUSHIKI KAISHA

发明人： HOSOI, Shinji ,  MIOH, Hiroyuki ,  SATO, Seiji ,  FUJIYOSHI, Nobuo

IPC分类号： C12N5/00 ,  C12N5/02

CPC分类号： C12M27/02 ,  C12M23/34

摘要： Improved process for multiplication on animal cells, which comprises culturing animal cells in a medium containing blood serum or a cell multiplication factor in one section separated from another section by a partition wall, and conducting perfusion culturing in a medium not containing serum nor cell multiplication factor in the another section. Substances useful as medicines can by yielded in large amounts by multiplication of the obtained animal cells.

摘要翻译： 动物细胞的繁殖方法，其中将动物细胞在分成两部分的培养基（例如通过膜，多孔壁或微胶囊化）中培养，一部分的培养基。 血清和/或细胞增殖因子，另一方面不限于此。 这些，灌注后者。 膜可以是M.W. 3000-100000的合成树脂; 多孔壁可以是金属烧结体或陶瓷; 微胶囊化可以是藻酸，琼脂糖或k-卡拉海南。 细胞增殖因子可以是肽或蛋白质（例如白细胞介素-2，生长调节素，皮肤细胞生长因子，血小板生长因子或α或β肿瘤细胞衍生的生长因子）。

公开(公告)号：EP0283521A1

公开(公告)日：1988-09-28

申请号：EP87906220.6

申请日：1987-09-28

申请人： KYOWA HAKKO KOGYO KABUSHIKI KAISHA

发明人： SAITO, Hiromitsu ,  UOSAKI, Yoichi ,  SATO, Akira ,  HIRATA, Tadashi ,  MORIMOTO, Makoto ,  ASHIZAWA, Tadashi

IPC分类号： C07D471/18 ,  C07D498/22 ,  A61K31/495

CPC分类号： C07D471/18 ,  C07D498/22

摘要： Novel DC-52 derivatives represented by formula (I). These compounds and their pharmaceutically acceptable salts have an excellent oncostatic activity. In the formula, X represents chlorine, bromine, iodine, hydroxy, formyl, hydroxyiminomethyl, cyano, nitro, amino or lower alkanolyamine, Y represents hydroxy, and Z represents cyano, or Y and Z are bound to each other to represent -O- as -Y-Z-.

摘要翻译： 05-52派生。 式（I）化合物及其药学上可接受的盐是新的，其中X是Cl，Br，I，OH，甲酰基，氰基，硝基，-CH = NOH，氨基或（低级烷酰基） Y是OH，Z是CN，或Y和Z一起是-O-。 已知衍生物的卤化。 DX-52-1（I，X = H，Y = OH，Z = CN）得到（I，X =卤素）; DX-52-1与路易斯酸/甲氧基二氯甲烷的反应得到（I，X = CHO）。 （I X = CHO）可以通过标准方法转化为肟，然后转化为X = CN; （I，X = CHO）上的Baeyer-Villiger给出（I，X = OH）。 硝化和还原DX-52-1得到（I，X =硝基和氨基）; （I，X =氨基）可以被酰化。 （I，Y = OH，Z = CN）可以通过水解/脱羧或使用银盐转化为（I，Y + Z = O）。