公开(公告)号：EP2724144A4

公开(公告)日：2015-03-11

申请号：EP12826157

申请日：2012-08-24

申请人： SONY CORP ,  SONY CORP AMERICA

发明人： DURACK GARY ,  FLEISCHER STEPHEN D ,  HATCHER JEREMY ,  ROBERTS DAVID ,  ZORDAN MICHAEL

IPC分类号： G01N21/00 ,  G01N15/14

CPC分类号： G01N15/1404 ,  G01N15/1427 ,  G01N15/1429 ,  G01N15/1459 ,  G01N2015/1006 ,  G01N2015/1075 ,  G01N2015/1415 ,  G01N2015/1438 ,  G01N2015/1477 ,  G01N2015/149

摘要： Apparatus and methods for detecting, characterizing, and compensating motion-related error of moving micro-entities are described. Motion-related error may occur in streams of moving micro-entities, and may represent a deviation in and expected arrival time or an uncertainty in position of a micro-entity within the stream. Motion-related error of micro-entities is observed in a flow cytometer, e.g., as pulse jitter, and is found to have a functional dependence on a parameter of the system. The pulse jitter can be compensated, according to one embodiment, by adjusting data acquisition observation windows. For the flow cytometer, a reduction of pulse jitter can improve measurement accuracy, resolution of doublets, system throughput, and enable an increase in an interrogation region for probing the micro-entities.

公开(公告)号：EP2696190A4

公开(公告)日：2014-09-24

申请号：EP13768656

申请日：2013-02-13

申请人： SONY CORP

发明人： MURAKI YOSUKE

IPC分类号： G01N15/14

CPC分类号： G01N15/1404 ,  B03C7/003 ,  B03C7/12 ,  B07C5/02 ,  B07C5/344 ,  G01N15/1484 ,  G01N2015/1415 ,  G01N2015/149 ,  G06T7/0012

摘要： There is provided a microparticle sorting device that automatically optimizes a fluid stream. There is provided a microparticle sorting device that includes a voltage supply unit that supplies a driving voltage to a vibratory element that applies vibration to an orifice that produces a fluid stream, a charge unit that imparts charge to at least some droplets ejected from the orifice, deflecting plates, arranged opposing each other with the fluid stream S therebetween, that vary a travel direction of the droplets, and a first image sensor that acquires an image of the droplets passing between the deflecting plates. The microparticle sorting device is equipped with a controller that detects the droplets in the image, sets a standard band corresponding to a width of the droplets before imparting the charge, and controls the driving voltage of the voltage supply unit so as to further decrease a quantity of the droplets detected in areas within a designated number of pixels from the standard band from among the droplets after imparting the charge.

公开(公告)号：EP2619545A2

公开(公告)日：2013-07-31

申请号：EP11827272.3

申请日：2011-09-16

申请人： The Regents of the University of California

发明人： DI CARLO, Dino ,  GOSSETT, Daniel R. ,  TSE, Henry T.K.

IPC分类号： G01N15/10 ,  G01N21/29 ,  G01N33/48 ,  G01N35/08

CPC分类号： G01N15/1463 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502776 ,  B01L2200/0605 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2300/0654 ,  B01L2300/0864 ,  C12Q1/02 ,  G01N15/1404 ,  G01N15/1459 ,  G01N15/1484 ,  G01N33/487 ,  G01N33/574 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1415 ,  G01N2015/149 ,  G01N2015/1495 ,  G01N2015/1497 ,  G01N2800/7028 ,  G06F19/10

摘要： A system is disclosed that enables the automated measurement of cellular mechanical parameters at high throughputs. The microfluidic device uses intersecting flows to create an extensional flow region where the cells undergo controlled stretching. Cells are focused into streamlines prior to entering the extensional flow region. In the extensional region, each cell's deformation is measured with an imaging device. Automated image analysis extracts a range of independent biomechanical parameters from the images. These may include cell size, deformability, and circularity. The single cell data that is obtained may then be used to in a variety of ways. Scatter density plots of deformability and circularity may be developed and displayed for the user. Mechanical parameters such as deformability and circularity may be gated or thresholded to identify certain cells of interest or sub-populations of interest. Similarly, the mechanical data obtained using the device may be used as cell signatures.

公开(公告)号：EP2180307A3

公开(公告)日：2012-10-03

申请号：EP10001278.0

申请日：2000-11-29

申请人： XY, LLC

发明人： Buchanan, Kristopher S. ,  Herickhoff, Lisa

IPC分类号： G01N15/14

CPC分类号： G01N15/1404 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1409 ,  G01N2015/1413 ,  G01N2015/1415 ,  G01N2015/149 ,  Y10T436/101666

摘要： An improved nozzle system for a flow cytometer have been invented for a high efficiency orientation and sorting process of a flat sample and dedicates items such as equine or bovine sperm cells. This improved nozzle system comprises a nozzle (16) with a novel interior surface geometry that can both gently accelerate the cells and can include an elliptical-like, single torsional interior surface element within (c) the nozzle, i.e., a single torsional orientation nozzle (6). The elliptical-like, single torsional interior surface element (e.g.) (8, 9, 10) may have a laminar flow surface and may produce the simplest flow path for applying minimal forces which act in either an accelerative nature or orienting hydrodynamic forces, namely, the single torsional orientation forces, to orient a flat sample (16) such as animal sperm cells into a proper direction for an analyzing and efficiently sorting process in clinical use, for research and for the animal insemination industry.

公开(公告)号：EP2479552A1

公开(公告)日：2012-07-25

申请号：EP11194709.9

申请日：2008-04-02

申请人： Acoustic Cytometry Systems, Inc.

发明人： Kaduchak, Gregory ,  Ward, Michael

IPC分类号： G01N15/14 ,  G01N29/22

CPC分类号： G01N15/1404 ,  A61M1/3678 ,  G01N15/12 ,  G01N15/147 ,  G01N21/1702 ,  G01N21/64 ,  G01N21/6428 ,  G01N21/645 ,  G01N27/447 ,  G01N29/222 ,  G01N33/56966 ,  G01N2015/1409 ,  G01N2015/1415 ,  G01N2015/142 ,  G01N2291/02416 ,  G01N2291/02809 ,  G01N2291/02836 ,  Y10T436/101666 ,  Y10T436/25 ,  Y10T436/25375 ,  Y10T436/25625 ,  Y10T436/2575

摘要： A system and method for analyzing one or more particles in a flow cell comprises focusing the one or more particles in a fluid stream with an acoustic standing wave to acoustically focus the one or more particles in a flow cell. The fluid is transited within the flow cell with a linear flow velocity ranging between about 0 meters/second and about 10 meters/second in the forward or reverse direction.

公开(公告)号：EP2275799A1

公开(公告)日：2011-01-19

申请号：EP10183127.9

申请日：2004-08-13

申请人： Luminex Corporation

发明人： Roth, Wayne D. ,  Moore, Douglas E.

IPC分类号： G01N15/14

CPC分类号： G01N15/1404 ,  G01N15/1012 ,  G01N21/645 ,  G01N2015/1415 ,  G01N2015/1477 ,  G01N2201/12746

摘要： A method for controlling one or more parameters of a flow cytometer type measurement system, is disclosed. The comprises the following steps
- measuring a time in which a microsphere (10) travels from a first detection window of the flow cytometer type measurement system to a second detection window of the measurement system; and
- altering an applied pressure of the measurement system such that the time is substantially constant.

摘要翻译： 一种用于控制流式细胞计型测量系统的流程的一个或多个参数的方法，是游离缺失盘。 该方法包括以下步骤 - 测量时间，其中，微球（10）从流流式细胞计型测量系统的测量系统的第二探测窗口的第一检测窗口行进; 和 - 检测所述测量系统的施加压力的改变做的时间基本上是恒定的。

公开(公告)号：EP2264430A2

公开(公告)日：2010-12-22

申请号：EP10182264.1

申请日：2000-11-29

申请人： XY, LLC

发明人： Buchanan, Kristopher S. ,  Herickhoff, Lisa

IPC分类号： G01N15/14

CPC分类号： G01N15/1404 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1409 ,  G01N2015/1413 ,  G01N2015/1415 ,  G01N2015/149 ,  Y10T436/101666

摘要： A flow cytometer system, comprising a sample injection tube having an injection point through which a sample may be introduced, a sheath fluid container having a bottom end and wherein said sample injection tube is located within said sheath fluid container, a sheath fluid port connected to said sheath fluid container, a first axial motion surface in a nozzle, a second axial motion surface in said nozzle, a limited maximal acceleration differentiation transition area between said first axial motion surface in said nozzle and said second axial motion surface in said nozzle wherein said limited maximal acceleration differentiation transition area is coordinated with said sample so as to be affirmatively limited to not exceed the practical capabilities of said sample over its length and an analytical system which senses below said nozzle.

摘要翻译： 一种流式细胞仪系统，包括具有注入点的样本注入管，样本可以通过该注入点引入;鞘液流体容器，具有底端，并且其中所述样本注入管位于所述鞘液流体容器内;鞘流体端口，连接到 所述鞘液容器，喷嘴中的第一轴向运动表面，所述喷嘴中的第二轴向运动表面，所述喷嘴中的所述第一轴向运动表面和所述喷嘴中的所述第二轴向运动表面之间的有限的最大加速度区分过渡区，其中所述 有限的最大加速度区分过渡区域与所述样本协调，以便被肯定地限制为不超过所述样本在其长度上的实际能力以及在所述喷嘴下面感测的分析系统。

公开(公告)号：EP2232212A2

公开(公告)日：2010-09-29

申请号：EP08868972.4

申请日：2008-12-19

申请人： Los Alamos National Security, LLC

发明人： KADUCHAK, Gregory ,  WARD, Michael, D.

IPC分类号： G01J3/00

CPC分类号： G01N29/14 ,  B01L3/502761 ,  B01L2200/0636 ,  B01L2200/0652 ,  B01L2300/0838 ,  B01L2300/0864 ,  B01L2400/0439 ,  B01L2400/0487 ,  G01N15/1404 ,  G01N15/1459 ,  G01N15/1463 ,  G01N33/4833 ,  G01N2001/4094 ,  G01N2015/1081 ,  G01N2015/1415 ,  G01N2015/142 ,  G01N2015/149

摘要： The present invention is a method and apparatus for acoustically manipulating one or more particles. Acoustically manipulated particles may be separated by size. The particles may be flowed in a flow stream and acoustic radiation pressure, which may be radial, may be applied to the flow stream. This application of acoustic radiation pressure may separate the particles. In one embodiment, the particles may be separated by size, and as a further example, the larger particles may be transported to a central axis.

公开(公告)号：EP2156178A1

公开(公告)日：2010-02-24

申请号：EP08733084.1

申请日：2008-04-02

申请人： Acoustic Cytometry Systems, Inc.

发明人： KADUCHAK, Gregory ,  WARD, Michael, D.

IPC分类号： G01N33/48

CPC分类号： G01N15/1404 ,  A61M1/3678 ,  G01N15/12 ,  G01N15/147 ,  G01N21/1702 ,  G01N21/64 ,  G01N21/6428 ,  G01N21/645 ,  G01N27/447 ,  G01N29/222 ,  G01N33/56966 ,  G01N2015/1409 ,  G01N2015/1415 ,  G01N2015/142 ,  G01N2291/02416 ,  G01N2291/02809 ,  G01N2291/02836 ,  Y10T436/101666 ,  Y10T436/25 ,  Y10T436/25375 ,  Y10T436/25625 ,  Y10T436/2575

摘要： A system and method for analyzing one or more particles in a flow cell comprises focusing the one or more particles in a fluid stream with an acoustic standing wave to acoustically focus the one or more particles in a flow cell. The fluid is transited within the flow cell with a linear flow velocity ranging between about 0 meters/second and about 10 meters/second in the forward or reverse direction.

公开(公告)号：EP2089723A2

公开(公告)日：2009-08-19

申请号：EP07873852.3

申请日：2007-11-13

申请人： University of Wyoming

发明人： JOHNSON, Paul, E.

IPC分类号： G01N35/10 ,  G01N15/14 ,  G01N1/00

CPC分类号： G01N35/1095 ,  G01N1/14 ,  G01N15/1404 ,  G01N21/05 ,  G01N21/64 ,  G01N2015/1415

摘要： Apparatus and methods for measuring the fluorescence of large multi-cellular organisms in a sample (102, 804) of liquid includes a pumping mechanism, a fluorescence measuring device (104), a method of analyzing (100, 300, 508) the measurements, and optionally, a sorting mechanism (502). The pumping mechanism transfers large multi-cellular organisms from a reservoir (402, 836) through a fluorescence-measuring device (104) causing minimum physical damage and/or stress. The pressure differential driving the organisms from a sample container/reservoir through the measuring device can be derived from gravity (800), air pressure (412), or liquid pressure, or some combination of the three. The fluorescence can be measured in a cytometer using a light detector or imager (106). Generally the detection element will include a filter (114), isolating the wavelength of fluorescent emission. The illumination may be provided by a laser (112) or by an LED (302), combined with the use of dichroic mirrors to allow multiple wavelength simultaneous illumination. In addition, an LED will typically be used with a filter to isolate the optimum excitation wavelength while blocking scattered/reflected LED light from the detection element (but not the fluorescence).