公开(公告)号：EP2812428B1

公开(公告)日：2018-07-25

申请号：EP13712360.0

申请日：2013-02-05

申请人： Centre National de la Recherche Scientifique

发明人： BAZOU, Despina ,  HOYOS, Mauricio ,  CASTRO CAMACHO, Luz Angelica

IPC分类号： C12N5/02 ,  G01N1/40 ,  G01N15/14

CPC分类号： C12N5/0062 ,  C12N5/0693 ,  C12N2513/00 ,  C12N2521/10 ,  G01N2001/4094 ,  G01N2015/142

摘要： A method of forming a multilayer aggregate (120) of objects (O) in a channel (2) comprising a liquid (L), said method comprising: a) providing objects (O) at first (50) and second (60) superposed regions of the channel (2), b) obtaining first (110) and second (111) aggregates of objects (O), optionally by applying transverse acoustic waves, preferably stationary waves, within each region to objects (O), and c) bringing said first (110) and second (111) aggregates into contact to form said multilayer aggregate (120) of objects (O) by submitting said first (110) and second (111) aggregates to: o gravity in absence of acoustic waves, or to o acoustic waves, optionally stationary waves, inducing displacement of said first (110) and second (111) aggregates toward each other.

公开(公告)号：EP3259596A1

公开(公告)日：2017-12-27

申请号：EP16707321.2

申请日：2016-02-16

申请人： Flodesign Sonics Inc.

发明人： GILMANSHIN, Rudolf ,  LIPKENS, Bart

IPC分类号： G01N33/543 ,  B01L3/00

CPC分类号： C07K1/045 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/12 ,  B01L2400/0436 ,  C07K16/00 ,  C12P19/34 ,  G01N1/31 ,  G01N15/1459 ,  G01N33/54313 ,  G01N2001/4094 ,  G01N2015/1006

摘要： Described herein are microreactors and methods of modifying support particles using acoustic standing waves. A liquid medium containing suspended support particles is flowed along a flow path through a channel, and an acoustic standing wave is applied to the channel to hold the suspended particles at a point in the channel. The held particles are then subjected to one or more reactions by flowing biochemical reactants through the channel. Unbound biological reactant is optionally washed from the channel. The reacted support particles can be released from the acoustic standing wave for further processing using, for example, flow cytometry or fluorescence microscopy.

公开(公告)号：EP3087194A4

公开(公告)日：2017-11-01

申请号：EP14874631

申请日：2014-12-11

申请人： BECTON DICKINSON CO

发明人： WARNER BRIAN DAVID ,  YU LIPING ,  GHANEKAR SMITA ,  CAO JIANYING

IPC分类号： G01N1/28 ,  B01L3/00 ,  C12Q1/02 ,  G01N1/40 ,  G01N15/10 ,  G01N21/64

CPC分类号： G01N1/286 ,  B01L3/502753 ,  B01L3/502776 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0487 ,  G01N2001/4094 ,  G01N2015/1006 ,  G01N2015/1418

摘要： Aspects of the present disclosure include methods for processing a biological sample. Methods according to certain embodiments include disrupting a biological sample to produce a disrupted biological sample and acoustically separating larger components from smaller components in the disrupted biological sample. In certain embodiments, methods may include monitoring aggregation while the biological sample is being processed. Methods, in certain instances, also include acoustically separating cells from cellular debris and non-cellular macromolecules as well as magnetically separating magnetically labelled moieties from unlabeled moieties. Systems, including a disrupter, one or more acoustic concentrator devices, feedback monitors and magnetic separation devices suitable for practicing the subject methods are also described.

公开(公告)号：EP3220131A1

公开(公告)日：2017-09-20

申请号：EP17161401.9

申请日：2017-03-16

申请人： COMMISSARIAT À L'ÉNERGIE ATOMIQUE ET AUX ÉNERGIES ALTERNATIVES

发明人： AUBERT, Vivian ,  CUBIZOLLES, Myriam-Laure ,  POULAIN, Cédric ,  HUET, Maxime ,  RABAUD, David

IPC分类号： G01N21/17 ,  G01N21/59 ,  G01N21/78 ,  G01N15/06 ,  G01N15/14 ,  G01N33/66 ,  C12Q1/54 ,  G01N21/27 ,  G01N1/40 ,  G01N21/80

CPC分类号： G01N21/1717 ,  G01N1/44 ,  G01N15/06 ,  G01N21/03 ,  G01N21/274 ,  G01N21/51 ,  G01N21/59 ,  G01N21/78 ,  G01N21/80 ,  G01N33/49 ,  G01N33/66 ,  G01N33/84 ,  G01N2001/4094 ,  G01N2015/0065 ,  G01N2015/0687 ,  G01N2015/0693 ,  G01N2021/1723 ,  G01N2021/1729 ,  G01N2021/1761

摘要： L'invention est un procédé de caractérisation d'un échantillon liquide (20), ledit échantillon comportant des particules baignant dans un milieu liquide (22), le procédé comportant les étapes suivantes :
a) illumination dudit échantillon à l'aide d'une source de lumière (11), apte à émettre une onde lumineuse incidente (12) vers l'échantillon ;
b) détection, à l'aide d'un photodétecteur (30), d'une onde lumineuse (14) transmise par l'échantillon ainsi illuminé ;
c) caractérisation de l'échantillon en fonction d'une intensité de l'onde lumineuse détectée par le photodétecteur.
Le procédé comprend, préalablement à l'étape c), une application d'une onde acoustique (45) dans l'échantillon, ladite onde acoustique formant des noeuds de pression (46) et des ventres de pression (47) dans l'échantillon, de manière à séparer, dans ce dernier, une partie appauvrie en particules et une partie enrichie en particules, l'étape c) comportant la caractérisation du milieu liquide à partir de l'intensité de l'onde lumineuse transmise par la partie appauvrie.

摘要翻译： 本发明是一种用于表征液体样品（20）的方法，所述样品包括浸入液体介质（22）中的颗粒，所述方法包括以下步骤：a）借助于 光源（11），适于向所述样品发射入射光波（12）; b）借助于光检测器（30）检测由如此照射的样品透射的光波（14）; c）将样品表征为由光电检测器检测到的光波强度的函数。 该方法包括在步骤c）之前在样本中施加声波（45），样本中的所述声波形成压力节点（46）和压力腹部（47） ，以便在后者中分离颗粒耗尽部分和富含颗粒的部分，阶段c）包括从耗尽部分透射的光波的强度表征液体介质。

公开(公告)号：EP2607881B1

公开(公告)日：2017-02-22

申请号：EP11195044.0

申请日：2011-12-21

申请人： Grundfos Holding A/S

发明人： Andersen Gad, Jens ,  Enemark, Heidi L. ,  Al-Sabi, Mohammad Nafi Solaiman ,  Kurtzhals, Jørgen

IPC分类号： G01N1/40

CPC分类号： G01N1/4077 ,  G01N2001/4088 ,  G01N2001/4094

公开(公告)号：EP2607881A1

公开(公告)日：2013-06-26

申请号：EP11195044.0

申请日：2011-12-21

申请人： Grundfos Holding A/S

发明人： Andersen Gad, Jens ,  Enemark, Heidi L. ,  Al-Sabi, Mohammad Nafi Solaiman ,  Kurtzhals, Jørgen

IPC分类号： G01N1/40

CPC分类号： G01N1/4077 ,  G01N2001/4088 ,  G01N2001/4094

摘要： A concentration device (2) for filter filtration concentration of particles (4) from a volume of a fluid (6). The concentration device (2) comprises a filter (8) configured to filter particles (4) of a predefined size in the volume of the fluid (6). The concentration device (2) comprises an ultrasonic transducer (10) that is configured to clean the filter (8).

摘要翻译： 一种用于从流体（6）的体积过滤过滤颗粒（4）浓度的浓缩装置（2）。 浓缩装置（2）包括过滤器（8），其配置成过滤流体（6）的体积中预定尺寸的颗粒（4）。 浓缩装置（2）包括被配置为清洁过滤器（8）的超声波换能器（10）。

公开(公告)号：EP2348322A2

公开(公告)日：2011-07-27

申请号：EP11151366.9

申请日：2011-01-19

申请人： SYSMEX CORPORATION

发明人： Ebi, Ryuichiro ,  Tajima, Koki

IPC分类号： G01N35/04 ,  G01N1/38

CPC分类号： G01N35/04 ,  B01F3/1242 ,  B01F11/0266 ,  B01F11/0283 ,  B01F2003/0042 ,  G01N1/38 ,  G01N15/14 ,  G01N15/1459 ,  G01N2001/4094 ,  G01N2015/1006 ,  G01N2015/149 ,  G01N2015/1493 ,  G01N2035/00465 ,  G01N2035/00554

摘要： A sample preparation apparatus, comprising: an ultrasonic vibrating u nit which applies ultrasonic vibration to a sample including an analyte held in a measurement sample container; a sample preparation unit which prepares a measurement sample by mixing the sample including the analyte to which th e ultrasonic vibration is applied and a predetermined reagent; and a container transporting unit which transports the measurement sample container holding the sample including the analyte from the ultrasonic vibrating unit to the sampl e preparation unit.

摘要翻译： 一种样品制备装置，包括：超声波振动装置，其对包含保存在测量样品容器中的分析物的样品施加超声波振动; 样品制备单元，其通过将包括施加超声波振动的分析物的样品与预定试剂混合来制备测量样品; 以及容器搬运单元，其将将包含分析物的样本的测量样本容器从超声波振动单元传送到样品制备单元。

公开(公告)号：EP1377811B1

公开(公告)日：2008-07-16

申请号：EP02719428.1

申请日：2002-04-03

申请人： Micronics, Inc.

发明人： WEIGL, Bernard, H. ,  BARDELL, Ronald, L. ,  BATTRELL, C., Frederick

IPC分类号： G01N15/14

CPC分类号： G01N15/05 ,  A61M1/14 ,  A61M2206/11 ,  B01D21/0012 ,  B01D21/283 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502746 ,  B01L3/502753 ,  B01L3/502761 ,  B01L3/502776 ,  B01L2200/027 ,  B01L2200/028 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/0829 ,  B01L2300/0861 ,  B01L2300/0874 ,  B01L2300/0883 ,  B01L2400/0406 ,  B01L2400/0436 ,  B01L2400/0457 ,  B01L2400/0487 ,  B01L2400/084 ,  F16K7/17 ,  F16K99/0001 ,  F16K99/0015 ,  F16K99/0025 ,  F16K99/0059 ,  F16K2099/008 ,  F16K2099/0084 ,  G01N15/0255 ,  G01N15/1456 ,  G01N2001/4016 ,  G01N2001/4061 ,  G01N2001/4094 ,  G01N2015/0288 ,  G01N2015/1411 ,  G01N2015/1413 ,  G01N2015/144 ,  G01N2015/1486 ,  G01N2035/00247 ,  Y10T436/25375 ,  Y10T436/2575

摘要： A microcytometer which combines lysing and cytometry into a unified system that achieves blood lysis and white blood cell count in a single device. The device focuses the white cells into a thin ribbon which is then focused into a single stream for analysis.

公开(公告)号：EP1774283A2

公开(公告)日：2007-04-18

申请号：EP05814034.4

申请日：2005-07-27

申请人： The Regents of the University of California

发明人： KADUCHAK, Gregory ,  GODDARD, Greg ,  SALZMAN, Gary ,  SINHA, Dipen ,  MARTIN, John C. ,  KWIATKOWSKI, Christopher ,  GRAVES, Steven

IPC分类号： G01N15/06

CPC分类号： B01D21/28 ,  G01N15/1459 ,  G01N2001/4094 ,  G01N2015/1413 ,  G01N2015/142 ,  Y10T137/0324 ,  Y10T137/206

摘要： The present invention includes an apparatus and corresponding method for concentrating analytes within a fluid flowing through a tube using acoustic radiation pressure. The apparatus includes a function generator that outputs a radio frequency electrical signal to a transducer that transforms the radio frequency electric signal to an acoustic signal and couples the acoustic signal to the tube. The acoustic signal is converted within the tube to acoustic pressure that concentrates the analytes within the fluid.

公开(公告)号：EP2820393B1

公开(公告)日：2017-12-06

申请号：EP12716598.3

申请日：2012-02-27

申请人： Centre National de la Recherche Scientifique ,  Ecole Supérieure de Physique et de Chimie Industrielles de la Ville de Paris

发明人： HOYOS, Mauricio ,  CASTRO CAMACHO, Luz Angelica

IPC分类号： G01N15/00 ,  B01F5/00 ,  C12N5/00

CPC分类号： G01N1/4077 ,  C12N5/0062 ,  C12N2521/10 ,  G01N2001/4094

摘要： A method of forming an aggregate of objects in a channel including a liquid, the method including: a) providing objects in at least a region of the channel, and b) forming an aggregate of the objects by submitting them to a modulated pulsed acoustic field, wherein the modulated pulsed acoustic field applied at step b) is modulated in amplitude.