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    Identification of genes altered in multiple myeloma
    85.
    发明公开
    Identification of genes altered in multiple myeloma 失效
    识别冯·Genen，多次死亡Myelomgeändertsind

    公开(公告)号：EP1731144A1

    公开(公告)日：2006-12-13

    申请号：EP06016570.1

    申请日：1997-05-28

    申请人： The Trustees of Columbia University in the City of New York

    发明人： Dalla-Favera, Riccardo

    IPC分类号： A61K31/00 ,  C07H21/02 ,  C07H21/04 ,  C07K2/00 ,  C07K14/435 ,  C07K16/18 ,  C12N15/00 ,  C12N15/09 ,  C12N15/63

    CPC分类号： C07K16/32 ,  A61K38/00 ,  C07K14/4702 ,  C07K14/82

    摘要： This invention provides a method of determining a chromosomal breakpoint in a subject suffering from multiple myeloma which comprises steps of: (a) obtaining a DNA sample from the subject suffering from multiple myeloma; (b) determining whether there is J and C disjunction in the immunoglobulin heavy chain gene in the obtained DNA sample; (c) obtaining a genomic library having clones which contain genomic DNA fragments from the DNA sample which shows positive J and C disjunction; (d) selecting and isolating clones of the obtained library which show positive hybridization with a probe which is capable of specifically hybridizing with the C but not the J region of the immunoglobulin heavy chain gene; (e) preparing fluorescent probes from the genomic DNA fragments of the isolated clones from step (d); (f) hybridizing said fluorescent probes with metaphase chromosomes; and (g) determining the identity of the chromosomes which are capable of hybridizing to said fluorescent probes, wherein the identification of a chromosome other than chromosome 14 would indicate that the chromosomal breakpoint is between chromosome 14 and the identified chromosome, thereby determining a chromosomal breakpoint in a subject suffering from multiple myeloma. This invention also provides the identified gene altered by a chromosomal breakpoint and various uses thereof.

    摘要翻译： 本发明提供了确定患有多发性骨髓瘤的受试者的染色体断裂点的方法，其包括以下步骤：（a）从患有多发性骨髓瘤的受试者获得DNA样品; （b）确定获得的DNA样品中免疫球蛋白重链基因中是否存在J和C分离; （c）获得具有克隆的基因组文库，所述克隆含有显示阳性J和C分离的DNA样品的基因组DNA片段; （d）选择和分离所获得的文库的克隆，其与能够与免疫球蛋白重链基因的C而不是J区特异性杂交的探针显示阳性杂交; （e）从步骤（d）的分离克隆的基因组DNA片段制备荧光探针; （f）将所述荧光探针与中期染色体杂交; 和（g）确定能够与所述荧光探针杂交的染色体的身份，其中除染色体14之外的染色体的鉴定将指示染色体断裂点在染色体14和所鉴定的染色体之间，由此确定染色体断点 在患有多发性骨髓瘤的受试者中。 本发明还提供了通过染色体断裂点改变的鉴定的基因及其各种用途。