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    DETERMINATION OF LIGHT ABSORPTION PATHLENGTH IN A VERTICAL-BEAM PHOTOMETER
    1.
    发明授权
    DETERMINATION OF LIGHT ABSORPTION PATHLENGTH IN A VERTICAL-BEAM PHOTOMETER 失效
    垂直光束测光仪中光吸收路径的测定

    公开(公告)号：EP0771417B1

    公开(公告)日：2009-09-16

    申请号：EP95928681.6

    申请日：1995-07-25

    申请人： MOLECULAR DEVICES CORPORATION

    发明人： HAFEMAN, Dean, G.

    IPC分类号： G01N21/25

    CPC分类号： G01N21/253 ,  G01N21/3151

    摘要： Disclosed are photometric methods and devices for determining optical pathlength of liquid samples containing analytes dissolved or suspended in a solvent. The methods and devices rely on determining a relationship between the light absorption properties of the solvent and the optical pathlength of liquid samples containing the solvent. This relationship is used to establish the optical pathlength for samples containing an unknown concentration of analyte but having similar solvent composition. Further disclosed are methods and devices for determining the concentration of analyte in such samples where both the optical pathlength and the concentration of analyte are unknown. The methods and devices rely on separately determining, at different wavelengths of light, light absorption by the solvent and light absorption by the analyte. Light absorption by the analyte, together with the optical pathlength so determined, is used to calculate the concentration of the analyte. Devices for carrying out the methods particularly advantageously include vertical-beam photometers containing samples disposed within the wells of multi-assays plates, wherein the photometer is able to monitor light absorption of each sample at multiple wavelengths, including in the visible or UV-visible region of the spectrum, as well as in the near-infrared region of the electromagnetic spectrum. Novel photometer devices are described which automatically determine the concentration of analytes in such multi-assay plates directly without employing a standard curve.

    摘要翻译： 公开了用于确定含有溶解或悬浮在溶剂中的分析物的液体样品的光程长度的光度测量方法和装置。 该方法和装置依赖于确定溶剂的光吸收性质和含有溶剂的液体样品的光程长度之间的关系。 该关系用于确定含有未知浓度分析物但具有相似溶剂组成的样品的光程长度。 还公开了用于测定这样的样品中分析物浓度的方法和装置，其中光路长度和分析物浓度都是未知的。 该方法和装置依赖于在不同波长的光下分别确定溶剂对光的吸收和被分析物的光吸收。 被分析物的光吸收以及如此确定的光程长度被用于计算分析物的浓度。 用于执行所述方法的设备特别有利地包括含有设置在多测定板的孔内的样品的垂直束光度计，其中该光度计能够监测多个波长处的每个样品的光吸收，包括在可见光或UV可见光区域 的光谱，以及电磁波谱的近红外区域。 描述了新的光度计装置，其直接自动确定这种多重测定板中分析物的浓度，而无需使用标准曲线。

    Fluorescent pH indicators for intracellular assays
    3.
    发明公开
    Fluorescent pH indicators for intracellular assays 审中-公开
    Fluoreszierende PH IndikatorenfürintrazelluläreAssays

    公开(公告)号：EP1281712A1

    公开(公告)日：2003-02-05

    申请号：EP02255414.1

    申请日：2002-08-01

    申请人： Molecular Devices Corporation

    发明人： Diwu, Zhenjun ,  Twu, Jesse J. ,  Yi, Guoliang ,  Lavis, Luke D. ,  Chen, Yen-Wen ,  Cassutt, Kelly J.

    IPC分类号： C07D493/10 ,  C07D311/82 ,  G01N33/84 ,  C12Q1/44

    CPC分类号： C07D493/10 ,  C07D311/82 ,  G01N1/30 ,  G01N33/84 ,  G01N2001/302 ,  G01N2800/04

    摘要： Systems, including compositions and methods, for measuring pH, particularly in cells, organelles, and other samples. The compositions include pH-sensitive fluorescent and fluorogenic 2',7'-dialkylfluorescein derivatives and associated nonfluorescent precursor compounds of the formula shown below. The compositions may permit ratiometric measurement in the excitation spectrum and the emission spectrum. The methods include adding a precursor compound to a sample cell, incubating the sample cell to release the free indicator, illuminating the sample cell, and detecting the fluorescence response of the free indicator.

    摘要翻译： 用于测量pH的系统，包括组合物和方法，特别是在细胞，细胞器和其它样品中。 组合物包括pH敏感的荧光和荧光2'，7'-二烷基荧光素衍生物和下列所示式的相关非荧光前体化合物。 组合物可以允许在激发光谱和发射光谱中进行比例测量。 所述方法包括向样品池中加入前体化合物，孵育样品池以释放游离指示剂，照射样品池，以及检测游离指示剂的荧光响应。