公开(公告)号：EP0773993A1

公开(公告)日：1997-05-21

申请号：EP95928825.0

申请日：1995-08-10

申请人： THE GENERAL HOSPITAL CORPORATION ,  BIOTRANSPLANT, INC

发明人： SACHS, David H. ,  SYKES, Megan ,  BAETSCHER, Manfred

IPC分类号： A01K67 ,  A61K35 ,  C07K14 ,  C12N5 ,  C12N15 ,  A61K38 ,  C12R1

CPC分类号： C12N15/8509 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2227/108 ,  A01K2267/01 ,  A01K2267/025 ,  A61K38/00 ,  C07K14/70503 ,  C07K14/71

摘要： A genetically engineered cell for inducing tolerance.

公开(公告)号：EP0669829A4

公开(公告)日：1997-05-07

申请号：EP93925071

申请日：1993-10-26

申请人： BIOTRANSPLANT INC

发明人： PONATH PAUL D ,  ROSA MARGARET D ,  MONROY RODNEY L ,  SCHACTER BERNICE Z ,  HAWLEY ROBERT J

IPC分类号： C12N15/09 ,  A61K35/28 ,  A61K38/00 ,  A61K38/19 ,  A61K39/00 ,  A61P37/06 ,  C07H21/04 ,  C07K14/475 ,  C07K14/52 ,  C07K14/535 ,  C07K14/54 ,  C12N5/10 ,  C12N15/19 ,  C12P21/02 ,  C12R1/19 ,  C12R1/91

CPC分类号： A61K35/28 ,  A61K38/00 ,  A61K39/00 ,  A61K39/001 ,  C07K14/475 ,  C07K14/52 ,  C07K14/535 ,  C07K14/54 ,  C07K2319/00 ,  Y10S514/885

摘要： A method of enhancing tolerance of a porcine transplant in a xenogeneic recipient by administering porcine bone marrow cells to the recipient and of enhancing proliferation and engraftment of the porcine bone marrow cells by exposing said cells to at least one substantially pure porcine cytokine and porcine cytokines that are substantially free of other porcine proteins and preferentially enhance the proliferation and engraftment of porcine bone marrow cells in the presence of bone marrow cells of other species. Protein and DNA sequence(s) for such porcine cytokines.

公开(公告)号：EP1210111A1

公开(公告)日：2002-06-05

申请号：EP00947121.0

申请日：2000-07-07

申请人： BIOTRANSPLANT, INC

发明人： PATIENCE, Clive

IPC分类号： A61K39/12 ,  C12N7/00

CPC分类号： C07K14/005 ,  A61K39/00 ,  C12N2710/16022 ,  C12N2710/16222

摘要： Isolated polynucleotides and polypeptides derived from the genome of swine gamma-herpesviruses are disclosed, including recombinant cells and vectors encoding such polypeptides and expressing such polynucleotides. Use of the novel polynucleotides as probes of the swine genome is also described. Assay methods employing antibodies against the isolated polypeptides are also disclosed.

公开(公告)号：EP0755402A1

公开(公告)日：1997-01-29

申请号：EP95915459.0

申请日：1995-03-31

申请人： BIOTRANSPLANT, INC ,  THE GENERAL HOSPITAL CORPORATION ,  INSTITUTE OF CHILD HEALTH

发明人： BAETSCHER, Manfred, W. ,  SACHS, David, H. ,  GUSTAFSSON, Kenth, T.

IPC分类号： A01K67 ,  C07H21 ,  C12N9 ,  C12N15 ,  A61K35

CPC分类号： C12N15/113 ,  A01K67/0275 ,  A01K67/0276 ,  A01K2217/05 ,  A01K2217/072 ,  A01K2217/075 ,  A01K2227/108 ,  A01K2267/02 ,  A01K2267/025 ,  A61K2035/122 ,  C12N9/1051 ,  C12N15/1137 ,  C12N15/8509 ,  C12N2310/111 ,  C12N2310/121 ,  C12N2310/315 ,  C12N2517/02 ,  C12N2830/52 ,  C12N2840/203 ,  C12Y204/01228

摘要： Transgenic swine in which the normal expression of alpha(1,3) galactosyltransferase is prevented in at least one organ of tissue type. The absence or inactivation of this enzyme prevents the production of carbohydrate moieties having the distinctive terminal Galalpha1-3Galbeta1-4GlcNAc epitope that is a significant factor in xenogeneic, particularly human, transplant rejection of swine grafts.

公开(公告)号：EP0706319A1

公开(公告)日：1996-04-17

申请号：EP94909464.0

申请日：1994-01-17

申请人： BIOTRANSPLANT, INC ,  THE GENERAL HOSPITAL CORPORATION

发明人： LE GUERN, Christian, A. ,  SEED, Brian ,  BANERJEE, Papia, T.

IPC分类号： C12N15 ,  A61K31 ,  A61K35 ,  A61K38 ,  A61K48 ,  A61P37 ,  C07K14 ,  C12N5 ,  C12N7 ,  C12P21 ,  C12R1

CPC分类号： C12N15/86 ,  C07K14/70539 ,  C12N15/79 ,  C12N2740/13043 ,  C12N2800/108 ,  C12N2840/203 ,  C12N2840/206 ,  C12N2840/44 ,  C12N2840/85

摘要： This invention relates to retroviral vectors that express multiple polypeptide subunits of a eukaryotic protein from a single polycistronic mRNA and the proteins produced from these vectors. The expressed proteins are particularly useful for inducing transplantation tolerance and the vectors are useful as agents for mediating gene transfer into eukaryotic cells.

公开(公告)号：EP1250594A4

公开(公告)日：2003-05-07

申请号：EP01903145

申请日：2001-01-19

申请人： BIOTRANSPLANT INC

发明人： PATIENCE CLIVE

IPC分类号： A01K67/02 ,  A01K67/027 ,  A61K35/12 ,  A61L27/00 ,  C12N5/06 ,  C12Q1/48 ,  C12Q1/68 ,  C12Q1/70 ,  G01N33/53 ,  G01N33/569 ,  G01N33/00 ,  A61K35/00 ,  C12N15/85 ,  C12P19/34

CPC分类号： A01K67/0271 ,  A01K67/027 ,  A61K35/12 ,  C12Q1/702

摘要： Miniature swine whose genomes contain sequences characteristic of pig endogenous retrovirus genes but which are non-infectious to humans are disclosed as sources of organs, tissues and cells for introduction into human recipients afflicted with diseases, or at risk of diseases, whose etiology involves the presence of inadequately functioning organs and for which xenotransplantation of such organs, tissues and cells would have a palliative effect. Methods of producing such animals and for screening animals for the desired properties are also disclosed.

公开(公告)号：EP0877941B1

公开(公告)日：2003-02-26

申请号：EP96943478.6

申请日：1996-11-05

申请人： BIOTRANSPLANT, INC ,  THE UNIVERSITY OF MIAMI

发明人： KENYON, Norma, S. ,  RUSSELL, Thomas, R. ,  RICORDI, Camillo ,  ZWERNER, Robert, K.

IPC分类号： G01N33/569 ,  G01N33/546 ,  G01N33/553

CPC分类号： G01N33/5375 ,  G01N33/54313 ,  G01N33/553 ,  G01N33/56966 ,  G01N33/56972

摘要： A cell population or subpopulation enrichment procedure (10) for separating undesired populations or subpopulations from a biological sample (12) utilizing relatively heavy, dense particles (14) and gravity sedimentation. The particles have one or more reactants bound thereto which are specific to and will bind with the selected population or subpopulation to be eliminated from the sample. The particles preferably are mixed with the sample by repeatedly causing the particles to settle through a substantial portion of the sample to bind to the selected population. The particles with the bound selected population to be eliminated then are allowed to preferentially settle in the sample and the supernatant including the enriched population or subpopulation is separated from the particles with the population to be eliminated bound thereto. The enriched population supernatant can then be analyzed, utilized as is, have other populations or subpopulations removed or have additional amounts of remaining cells of the previously eliminated populations removed in additional steps.

公开(公告)号：EP0669829B1

公开(公告)日：2001-08-08

申请号：EP93925071.8

申请日：1993-10-26

申请人： BIOTRANSPLANT, INC

发明人： PONATH, Paul, D. ,  ROSA, Margaret, D. ,  MONROY, Rodney, L. ,  SCHACTER, Bernice, Z. ,  HAWLEY, Robert, J.

IPC分类号： C12N15/19 ,  C12N15/62 ,  A61K35/28 ,  C07K14/52 ,  A61K38/19 ,  C12N15/85 ,  C12N5/10

CPC分类号： A61K35/28 ,  A61K38/00 ,  A61K39/00 ,  A61K39/001 ,  C07K14/475 ,  C07K14/52 ,  C07K14/535 ,  C07K14/54 ,  C07K2319/00 ,  Y10S514/885

摘要： A method of enhancing tolerance of a porcine transplant in a xenogeneic recipient by administering porcine bone marrow cells to the recipient and of enhancing proliferation and engraftment of the porcine bone marrow cells by exposing said cells to at least one substantially pure porcine cytokine and porcine cytokines that are substantially free of other porcine proteins and preferentially enhance the proliferation and engraftment of porcine bone marrow cells in the presence of bone marrow cells of other species. Protein and DNA sequence(s) for such porcine cytokines.

公开(公告)号：EP0773993A4

公开(公告)日：2000-07-05

申请号：EP95928825

申请日：1995-08-10

申请人： GEN HOSPITAL CORP ,  BIOTRANSPLANT INC

发明人： SACHS DAVID H ,  SYKES MEGAN ,  BAETSCHER MANFRED

IPC分类号： A01K67/027 ,  A61K35/12 ,  A61K38/00 ,  C07K14/705 ,  C07K14/71 ,  C12N5/00 ,  C12N5/10 ,  C12N15/09 ,  C12N15/85 ,  C12R1/91 ,  C12N15/00 ,  C07K14/715

CPC分类号： C12N15/8509 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2227/108 ,  A01K2267/01 ,  A01K2267/025 ,  A61K38/00 ,  C07K14/70503 ,  C07K14/71

摘要： A genetically engineered cell for inducing tolerance.

公开(公告)号：EP0969872A2

公开(公告)日：2000-01-12

申请号：EP98907366.3

申请日：1998-02-05

申请人： BIOTRANSPLANT, INC

发明人： THALL, Aron

IPC分类号： A61K47/48

CPC分类号： A61K9/1271 ,  A61K47/58 ,  A61K47/61 ,  A61K47/6825 ,  A61K47/6849

摘要： The invention provides methods and compositions for promoting in a first species a state of tolerance against Galα1,3Gal epitopes present on a xenograft from a second species, thereby preventing hyperacute rejection (HAR) of the xenograft. The methods and compositions according to the invention cause the elimination or anergy of specific lymphoid according cells which are responsible for the production of xenoreactive natural antibodies (XNAs) which cause HAR of the xenograft. In a first aspect, the invention provides methods and tolerogenic compositions for inducing anergy in B cells which are specific for the Galα1,3Gal epitope. In a second aspect, the invention provides methods and tolerogenic compositions for inducing apoptosis in B cells specific for the Galα1,3Gal epitope.