PROCESSES FOR THE PREPARATION OF CEPHALOSPORIN DERIVATIVES
    5.
    发明公开
    PROCESSES FOR THE PREPARATION OF CEPHALOSPORIN DERIVATIVES 审中-公开
    用于生产头孢菌素衍生物

    公开(公告)号:EP1678187A4

    公开(公告)日:2007-06-13

    申请号:EP04793628

    申请日:2004-10-30

    申请人: CJ CORP

    IPC分类号: C07D501/00 C07D501/22

    CPC分类号: C07D501/00 Y02P20/55

    摘要: Provided is a process for preparing a compound of formula 1 or its salt. The process includes reacting a compound of formula 4 with acetaldehyde in a mixed solvent including water, isopropanol, and methylenechloride in a volume ratio of 1:3-6:11-14 in the presence of a first base to stereospecifically prepare a compound of formula 3 and reacting the compound of the formula 3 with an anhydrous compound of formula 2 in the presence of a second base.

    GLYCOSYLATED HUMAN GRANULOCYTE COLONY-STIMULATING FACTOR (G-CSF) ISOFORM
    7.
    发明公开
    GLYCOSYLATED HUMAN GRANULOCYTE COLONY-STIMULATING FACTOR (G-CSF) ISOFORM 审中-公开
    糖基化的人粒细胞集落刺激因子的同种型(G-CSF)

    公开(公告)号:EP1551870A2

    公开(公告)日:2005-07-13

    申请号:EP03748751.9

    申请日:2003-08-29

    申请人: CJ Corp.

    IPC分类号: C07K14/47

    CPC分类号: C07K14/535 A61K38/00

    摘要: Disclosed are human G-CSF isoforms having a modified amino acid sequence containing a glycosylation sequence Asn-X-Ser/Thr (N-X-S/T) at one or more specific amino acid positions according to the present invention, genes encoding the human G-CSF isoforms, and expression vectors carrying the genes, eukaryotic cells transformed or transfected with the expression vectors. Also, the present invention discloses a method of preparing a glycosylated human G-CSF isoform, comprising the steps of culturing the transformant or transfectant and isolating a glycosylated human G-CSF isoform from the culture supernatant or cell lysates, a human G-CSF isoform prepared by the method, and a pharmaceutical composition comprising the human G-CSF isoform.