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    TSG-LIKE GENE
    2.
    发明公开
    TSG-LIKE GENE 审中-公开
    TSG-äHNLICHE基因

    公开(公告)号：EP1215284A1

    公开(公告)日：2002-06-19

    申请号：EP00956961.7

    申请日：2000-09-06

    申请人： CHUGAI SEIYAKU KABUSHIKI KAISHA ,  Kitamura, Toshio

    发明人： KITAMURA, Toshio ,  MORITA, Sumiyo

    IPC分类号： C12N15/12 ,  C07K14/475 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12P21/02 ,  C07K16/18 ,  C12Q1/68

    CPC分类号： C07K14/475 ,  A61K38/00

    摘要： A gene encoding a novel protein that is homologous to Drosophila TSG was isolated from a cDNA library derived from the AGM region of mouse embryos by using an originally developed cloning method specific to a gene encoding a membrane secretory protein. This gene is useful in developing drugs that regulate hematopoietic stem cell generation, immune and hematopoietic functions, etc.

    摘要翻译： 通过使用对编码膜分泌蛋白的基因特异的最初开发的克隆方法，从源自小鼠胚胎的AGM区域的cDNA文库中分离编码与果蝇TSG同源的新蛋白质的基因。 该基因可用于开发调节造血干细胞产生，免疫和造血功能等的药物。

    SIGNAL SEQUENCE TRAPPING METHOD
    9.
    发明公开
    SIGNAL SEQUENCE TRAPPING METHOD 有权
    VERFAHREN ZUM EINFANGEN VON SIGNALSEQUENZEN

    公开(公告)号：EP1033374A1

    公开(公告)日：2000-09-06

    申请号：EP98955942.2

    申请日：1998-11-26

    申请人： CHUGAI SEIYAKU KABUSHIKI KAISHA ,  Kitamura, Toshio

    发明人： KITAMURA, Toshio ,  KOJIMA, Tetsuo, Chugai Res. Inst. Molec. Med. Inc

    IPC分类号： C07K14/715 ,  C12N15/12 ,  C12N5/16 ,  C12Q1/68

    CPC分类号： C12N15/625 ,  C07K2319/02 ,  C07K2319/75 ,  C12N2799/027 ,  C12Q1/6897

    摘要： A DNA encoding human mpI lacking the secretory ability, prepared by deleting the secretory signal of homeostatically active mpl and the major part of the extracellular region thereof. This DNA is ligated with a test cDNA, a cDNA encoding a known secretory protein and another cDNA encoding the same protein from which the secretory signal region is removed, each of the resulting chimeric genes is expressed in cells to examine cell proliferation capability. Cell proliferation is detected in the cells containing the cDNA encoding the known secretory protein as a test cDNA, whereas no cell proliferation is detected for the cDNA encoding the same protein from which the secretory signal region is removed. Thus, cDNAs encoding secretory proteins including type I membrane proteins and type II membrane proteins can be detected and isolated by constructing a cDNA library and screening it by the above method.

    摘要翻译： 通过缺失平衡活性mpl的分泌信号及其细胞外区域的主要部分而制备的编码人mpI缺乏分泌能力的DNA。 将该DNA与测试cDNA连接，编码已知分泌蛋白的cDNA和编码与其分离信号区域相同的蛋白质的另一个cDNA，每个所得嵌合基因在细胞中表达以检测细胞增殖能力。 在含有编码已知分泌蛋白的cDNA的细胞中检测到细胞增殖作为测试cDNA，而对于从其中除去分泌信号区域的相同蛋白质的cDNA，未检测到细胞增殖。 因此，可以通过构建cDNA文库并通过上述方法进行筛选来检测和分离包括I型膜蛋白和II型膜蛋白的分泌蛋白的cDNA。