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    COMPOSITIONS AND METHODS FOR IDENTIFYING LATENTLY INFECTED CELLS
    5.
    发明公开
    COMPOSITIONS AND METHODS FOR IDENTIFYING LATENTLY INFECTED CELLS 审中-公开
    组成和程序，以识别潜伏感染细胞

    公开(公告)号：EP3033087A4

    公开(公告)日：2017-03-15

    申请号：EP14836825

    申请日：2014-08-14

    申请人： THE J DAVID GLADSTONE INST

    发明人： VERDIN ERIC M ,  CALVANESE VINCENZO

    IPC分类号： A61K31/551

    CPC分类号： C12N15/86 ,  A61K35/17 ,  C12N7/00 ,  C12N2740/16011 ,  C12N2740/16043 ,  C12N2830/60 ,  C12Q1/6897 ,  C12Q1/703

    摘要： The present disclosure provides for recombinant nucleic acids, and cells and virions comprising the recombinant nucleic acids, that can be used to identify, isolate, and/or purify cells latently infected with immunodeficiency virus. A subject recombinant nucleic acid includes (a) a first nucleotide sequence encoding a first reporter polypeptide that produces a first detectable signal, where the first nucleotide sequence is operably linked to an immunodeficiency virus promoter and is translated as an early gene; and (b) a second nucleotide sequence encoding a second reporter polypeptide that produces a second detectable signal that is distinguishable from the first detectable signal, where the second nucleotide sequence is operably linked to a non-immunodeficiency virus promoter. In some aspects, the first and second nucleotide sequences are both positioned between a shared 5′ long terminal repeat (LTR) and a shared 3′ LTR. Also provided are related methods.

    COMPOSITIONS, SYSTEMS AND METHODS FOR GENE EXPRESSION NOISE DRUG SCREENING AND USES THEREOF
    8.
    发明公开
    COMPOSITIONS, SYSTEMS AND METHODS FOR GENE EXPRESSION NOISE DRUG SCREENING AND USES THEREOF 审中-公开
    ZUSAMMENSETZUNGEN，SYSTEME UND VERFAHRENFÜRGENEXPRESSIONSRAUSCHEN-WIRKSTOFFSCREENING UND VERWENDUNGEN DAVON

    公开(公告)号：EP3052662A4

    公开(公告)日：2017-07-05

    申请号：EP14851396

    申请日：2014-10-01

    申请人： THE J DAVID GLADSTONE INST

    发明人： WEINBERGER LEOR ,  DAR ROY

    IPC分类号： C12Q1/70

    CPC分类号： G01N33/5023 ,  A61K31/22 ,  A61K31/496 ,  A61K38/191 ,  C12Q1/6897 ,  C12Q1/703 ,  G01N2333/16

    摘要： HIV-1's ability to enter a transcriptionally dormant state and establish a reservoir of latently infected cells is considered the major barrier to eradicating the virus from infected patients. Stochastic noise (i.e. fluctuations) in an HIV-1 transcriptional positive-feedback loop is one mechanism that enables HIV-1 to establish latency. Here, Applicants demonstrate that small-molecule modulation of noise in HIV-1 gene expression radically perturbs HIV-1 latency.

    摘要翻译： HIV-1进入转录休眠状态并建立潜伏感染细胞库的能力被认为是从感染患者中根除病毒的主要障碍。 HIV-1转录正反馈环中的随机噪声（即波动）是使HIV-1建立潜伏期的一种机制。 在此，申请人证明HIV-1基因表达中的小分子噪声调制从根本上扰乱了HIV-1潜伏期。