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    NUCLEASE INHIBITOR COCKTAIL
    3.
    发明公开
    NUCLEASE INHIBITOR COCKTAIL 有权
    COCKTAIL核酸酶

    公开(公告)号：EP1220944A1

    公开(公告)日：2002-07-10

    申请号：EP00966924.3

    申请日：2000-09-25

    申请人： AMBION, INC.

    发明人： WINKLER, Matthew, M. ,  KUDLICKI, W., Antoni ,  PASLOSKE, Brittan, L.

    IPC分类号： C12Q1/34 ,  C12Q1/68 ,  C12N9/00

    CPC分类号： C12N15/1003 ,  C07K16/40 ,  C12N15/1096 ,  C12Q1/34 ,  G01N33/573 ,  G01N2333/922

    摘要： Methods and compositions for inhibiting and/or inactivating nucleases by employing nuclease inhibitors are provided. The nuclease inhibitors comprise anti-nuclease antibodies and non-antibody nuclease inhibitors. The anti-nuclease antibodies of the present invention may be a polyclonal or monoclonal antibodies, and may be antiribonuclease antibodies, anti-deoxyribonuclease antibodies, or antibodies to a non-specific nucleases. A preferred embodiment comprises at least two nuclease inhibitors, and is referred to as a nuclease inhibitor cocktail. In some specific embodiments, the invention concerns methods of performing in vitro translation comprising obtaining a first nuclease inhibitor, which inhibitor is further defined as an anti-nuclease antibody, and placing the anti-nuclease antibody in an in vitro translation reaction. In many cases, the in vitro translation reaction comprises at least one nuclease, which may be a ribonuclease, a deoxyribonuclease, or a nonspecific nuclease. The invention also relates to kits for the performance of various microbiological procedures, which kits comprise the nuclease inhibitors described herein.

    COMPARATIVE ANALYSIS OF NUCLEIC ACIDS USING POPULATION TAGGING
    4.
    发明公开
    COMPARATIVE ANALYSIS OF NUCLEIC ACIDS USING POPULATION TAGGING 审中-公开
    与人口TAGGING NUCLEIC的比较分析

    公开(公告)号：EP1360333A2

    公开(公告)日：2003-11-12

    申请号：EP02720894.1

    申请日：2002-01-31

    申请人： AMBION, INC.

    发明人： WINKLER, Matthew, M. ,  BROWN, David

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6809 ,  C12Q2531/143 ,  C12Q2525/161 ,  C12Q2525/155

    摘要： Disclosed are methods that allow one or more nucleic acid targets to be compared across two or more nucleic acid samples. Nucleic acid tags are appended to the samples to be assessed, such that each sample has a unique tag. The tagged nucleic acids are then mixed, and the targets within the mixture are amplified. The amplification products are distinguished using the unique tag domains to reveal the abundance of the amplification products derived from each sample, which correlates to the relative abundance of the target in the samples.

    NUCLEASE INHIBITOR COCKTAIL
    6.
    发明授权
    NUCLEASE INHIBITOR COCKTAIL 有权
    COCKTAIL核酸酶

    公开(公告)号：EP1220944B1

    公开(公告)日：2010-09-15

    申请号：EP00966924.3

    申请日：2000-09-25

    申请人： Ambion, Inc.

    发明人： WINKLER, Matthew, M. ,  KUDLICKI, W., Antoni ,  PASLOSKE, Brittan, L.

    IPC分类号： C12Q1/34 ,  C12N9/00 ,  C07K16/40

    CPC分类号： C12N15/1003 ,  C07K16/40 ,  C12N15/1096 ,  C12Q1/34 ,  G01N33/573 ,  G01N2333/922

    摘要： Methods and compositions for inhibiting and/or inactivating nucleases by employing nuclease inhibitors are provided. The nuclease inhibitors comprise anti-nuclease antibodies and non-antibody nuclease inhibitors. The anti-nuclease antibodies of the present invention may be a polyclonal or monoclonal antibodies, and may be antiribonuclease antibodies, anti-deoxyribonuclease antibodies, or antibodies to a non-specific nucleases. A preferred embodiment comprises at least two nuclease inhibitors, and is referred to as a nuclease inhibitor cocktail. In some specific embodiments, the invention concerns methods of performing in vitro translation comprising obtaining a first nuclease inhibitor, which inhibitor is further defined as an anti-nuclease antibody, and placing the anti-nuclease antibody in an in vitro translation reaction. In many cases, the in vitro translation reaction comprises at least one nuclease, which may be a ribonuclease, a deoxyribonuclease, or a nonspecific nuclease. The invention also relates to kits for the performance of various microbiological procedures, which kits comprise the nuclease inhibitors described herein.