公开(公告)号：EP1618204A2

公开(公告)日：2006-01-25

申请号：EP04715777.1

申请日：2004-02-27

申请人： Bristol-Myers Squibb Company

发明人： SKONEZNY, Paul, M. ,  POLITINO, Michael ,  LIU, Suo, W. ,  BOYLE, Alfred, W. ,  CHEN, Jason, G. ,  STEIN, Gregory, L. ,  FRANCESCHINI, Thomas ,  ANDERSON, Wendy, L.

IPC分类号： C12Q1/00

CPC分类号： C07H19/167 ,  C12P19/40

摘要： A method of making didanosine (ddI) including the steps of: (a) obtaining an enzyme expressing ddA deaminase activity; (b) immobilizing the enzyme onto an insoluble support; (c) contacting the enzyme with a dideoxyadenosine (ddA) solution of at least about 4% weight volume ddA in water for a time and under conditions to produce a ddI solution; and (d) isolating the ddI from the ddI solution. Optionally, the ddI mother liquor is reused in subsequent runs to improve yield.

公开(公告)号：EP0990023A1

公开(公告)日：2000-04-05

申请号：EP98926385.0

申请日：1998-06-08

申请人： BRISTOL-MYERS SQUIBB COMPANY

发明人： LIU, Suo, W. ,  FRANCESCHINI, Thomas

IPC分类号： C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/81 ,  C07H21/04 ,  C12P21/00

CPC分类号： C12N9/0024 ,  C07K14/495 ,  C12N9/0012 ,  C12N9/16 ,  C12N9/18 ,  C12N9/86 ,  C12N15/81

摘要： A D-amino acid oxidases promoter isolated from T. variabilis has been incorporated into S. cerevisiae expression vectors to increase the expression of heterologous genes. The heterologous promoter has been placed upstream of selective markers, auxotrophic nutritional and/or dominant selectable markers. Because the promoter is from another yeast specie, it is weakly recognized by S. cerevisiae. To compensate for the weak transcripts of selectable marker genes transcribed from the weak heterologous promoter, the plasmid containing the gene of the interest is amplified leading to a higher expression of protein of interest.

公开(公告)号：EP1040184B1

公开(公告)日：2007-11-07

申请号：EP98962067.9

申请日：1998-12-11

申请人： Bristol-Myers Squibb Company

发明人： LIU, Suo, W. ,  FRANCESCHINI, Thomas

IPC分类号： C12N15/70 ,  C12N1/21 ,  C12N15/72 ,  C12P21/00

CPC分类号： C12N9/84 ,  C07K14/245 ,  C12N15/70 ,  C12N15/72

摘要： High expression vectors for expression of heterologous genes in Escherichia coli. The expression vectors contain, the tac promoter, an intergenic region, a restriction site, and, optionally, groES DNA.

公开(公告)号：EP1040184A1

公开(公告)日：2000-10-04

申请号：EP98962067.9

申请日：1998-12-11

申请人： BRISTOL-MYERS SQUIBB COMPANY

发明人： LIU, Suo, W. ,  FRANCESCHINI, Thomas

IPC分类号： C12N1/21 ,  C12N15/71 ,  C12N15/72 ,  C12P21/00

CPC分类号： C12N9/84 ,  C07K14/245 ,  C12N15/70 ,  C12N15/72

摘要： High expression vectors for expression of heterologous genes in Escherichia coli. The expression vectors contain, the tac promoter, an intergenic region, a restriction site, and, optionally, groES DNA.