公开(公告)号：EP1545752A2

公开(公告)日：2005-06-29

申请号：EP03752253.9

申请日：2003-09-09

申请人： Cytonome, Inc.

发明人： GILBERT, John, R. ,  DESHPANDE, Manish

IPC分类号： B01D63/00 ,  C02F1/44 ,  C12M1/34 ,  B01L11/00 ,  B01L3/00 ,  C23F1/00

CPC分类号： B01L3/502715 ,  B01L3/06 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2200/027 ,  B01L2200/028 ,  B01L2200/146 ,  B01L2300/041 ,  B01L2300/047 ,  B01L2300/0681 ,  B01L2400/0481 ,  B01L2400/0655 ,  B01L2400/0666 ,  C30B7/00 ,  C30B29/58

摘要： A system and method for integrating microfluidic components in a microfluidic system enables the microfluidic system to perform a selected microfluidic function. A capping module includes a microfluidic element for performing a microfluidic function. The capping module is stacked on a microfluidic substrate having microfluidic plumbing to incorporate the microfluidic function into the system.

公开(公告)号：EP1616189A2

公开(公告)日：2006-01-18

申请号：EP04749668.2

申请日：2004-03-31

申请人： Cytonome, Inc.

发明人： GILBERT, John, R. ,  DESHPANDE, Manish ,  TRIKHA, Jaishree

IPC分类号： G01N33/566 ,  B01D63/00 ,  B01L11/00 ,  C23F1/00 ,  C12Q1/00

CPC分类号： G01N30/84 ,  B01L3/502715 ,  B01L3/502753 ,  B01L2200/027 ,  B01L2200/028 ,  B01L2300/0681 ,  B01L2300/0887 ,  B01L2400/0421 ,  G01N30/02 ,  G01N30/6095 ,  G01N30/80 ,  G01N2030/8411 ,  B01D15/3804

摘要： A system and method for integrating microfluidic components in a microfluidic system enables the microfluidic system to perform a selected microfluidic function. A capping module includes a microfluidic element for performing a microfluidic function. The capping module is stacked on a microfluidic substrate having microfluidic plumbing to incorporate the microfluidic function into the system. The microfluidic element may comprise a matrix having an affinity for selected molecules in a sample. The matrix binds, reacts with and/or retains the selected molecules without affecting other molecules in the sample.

公开(公告)号：EP1499439A1

公开(公告)日：2005-01-26

申请号：EP03719665.6

申请日：2003-04-09

申请人： Cytonome, Inc.

发明人： GILBERT, John, R. ,  BÖHM, Sebastian

IPC分类号： B01L3/00

CPC分类号： B01L3/0248 ,  B01L3/0244 ,  B01L3/5088 ,  B01L9/543 ,  B01L2200/12 ,  B01L2200/142 ,  B01L2200/143 ,  B01L2200/147 ,  B01L2300/161 ,  B01L2300/1822 ,  B01L2400/0439 ,  G01N2035/1037 ,  Y10S977/888 ,  Y10T29/49 ,  Y10T436/2575

摘要： A cooled liquid sample dispensing system (10) comprises a pair of pins (11, 12) for holding a droplet (18) of liquid therebetween and a cooling element. (200) Each pin (11, 12) includes a tip (11a, 11b) spaced predetermined distance from the other pin (11, 12) to define a sample acquisition region (14). The pins (11, 12) acquire and hold a droplet (18) of the liquid sample in the sample acquisition region (14) formed in the space between the tips (11a, 12a) and apply the droplet (18) to a selected sample handing system. The cooling element (200), when activated, cools the droplet (18) of liquid to reduce evaporation.

摘要翻译： 冷却的液体样品分配系统（10）包括用于保持液体之间的液滴（18）的一对销（11,12）和冷却元件。 （200）每个销（11,12）包括与另一个销（11,12）隔开预定距离的尖端（11a，11b）以限定样本采集区域（14）。 销（11,12）在形成于尖端（11a，12a）之间的空间中的样本采集区域（14）中获取并保持液体样本的液滴（18），并将液滴（18）施加到选定的样本 处理系统。 冷却元件（200）在被激活时冷却液体的液滴（18）以减少蒸发。

公开(公告)号：EP1817568A2

公开(公告)日：2007-08-15

申请号：EP05853002.3

申请日：2005-12-05

申请人： Cytonome, Inc.

发明人： GILBERT, John, R. ,  LEWIS, Hugh ,  BEAUPRE, Derek ,  DESHPANDE, Manish ,  TRIKHA, Jaishree

IPC分类号： G01N15/10 ,  B01L3/00

CPC分类号： G01N33/577 ,  B01L3/502761 ,  B01L3/502776 ,  B01L5/00 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/082 ,  B01L2200/10 ,  B01L2300/0809 ,  B01L2300/0864 ,  B01L2400/0487 ,  C12M47/04 ,  G01N15/1056 ,  G01N15/1484 ,  G01N2015/1486 ,  G01N2015/149 ,  Y10T436/2575

摘要： A single disposable cartridge for performing a process on a particle, such as particle sorting, encapsulates all fluid contact surfaces in the cartridge for use with microfluidic particle processing technology. The cartridge interfaces with an operating system for effecting particle processing. The encapsulation of the fluid contact surfaces insures, improves or promotes operator isolation and/or product isolation. The cartridge may employ any suitable technique for processing particles.

公开(公告)号：EP1661165A2

公开(公告)日：2006-05-31

申请号：EP04786510.0

申请日：2004-08-16

申请人： Cytonome, Inc.

发明人： GILBERT, John, R. ,  SINOFSKY, Ed. ,  DESHPANDE, Manish

IPC分类号： H01L21/00

CPC分类号： G01N15/1434 ,  G01N15/1459 ,  G01N15/1484 ,  G01N21/6452 ,  G01N2015/1081

摘要： An optical system for acquiring fast spectra from spatially channel arrays includes a light source for producing a light beam that passes through the microfluidic chip or the channel to be monitored, one or more lenses or optical fibers for capturing the light from the light source after interaction with the particles or chemicals in the microfluidic channels, and one or more detectors. The detectors, which may include light amplifying elements, detect each light signal and transducer the light signal into an electronic signal. The electronic signals, each representing the intensity of an optical signal, pass from each detector to an electronic data acquisition system for analysis. The light amplifying element or elements may comprise an array of phototubes, a multianode phototube, or a multichannel plate based image intensifier coupled to an array of photodiode detectors.

公开(公告)号：EP1546672A1

公开(公告)日：2005-06-29

申请号：EP03752252.1

申请日：2003-09-09

申请人： Cytonome, Inc.

发明人： GILBERT, John, R. ,  TRIKHA, Jaishree

IPC分类号： G01N1/00 ,  G01N1/18

CPC分类号： B01D61/243 ,  B01D61/28 ,  B01D63/087 ,  B01D63/088 ,  B01L3/06 ,  B01L3/5027 ,  B01L2300/0681 ,  C30B7/00 ,  C30B29/58

摘要： A system for crystallizing a protein and other biomolecules, the general scheme of which is represented in Figure 1, comprises a flow channel (12) for conveying a solution containing the biomolecule to be crystallized and one or more substations (40, 50, 60, 70) related to the crystallization process formed along the flow channel. The system operates by flowing a biomolecule solution through the flow channel (12), then performing dialysis on a micro-scale to set crystallization conditions. After dialysis, the concentration of the biomolecule solution is changed by removing water or solvent from the biomolecule solution to promote formation of crystals in a segment of the flow channel. Formed crystals may be observed and harvested from the flow channel.

摘要翻译： 用于使蛋白质和其它生物分子结晶的系统（其总体方案如图1所示）包括用于输送含有待结晶的生物分子的溶液的流动通道（12）和一个或多个变电站（40,50,60,70,80,90） 70）与沿着流动通道形成的结晶过程有关。 该系统通过使生物分子溶液流过流动通道（12），然后在微尺度上进行透析以设定结晶条件来操作。 透析后，通过从生物分子溶液中除去水或溶剂来改变生物分子溶液的浓度，以促进在一部分流动通道中形成晶体。 可以观察到形成的晶体并从流动通道收获。

公开(公告)号：EP1682438A2

公开(公告)日：2006-07-26

申请号：EP04810241.2

申请日：2004-11-01

申请人： Cytonome, Inc.

发明人： GILBERT, John, R. ,  DESHPANDE, Manish ,  BUNNER, Bernard

IPC分类号： B65G51/00

CPC分类号： B65G51/08 ,  B01F13/0062 ,  B01F13/0064 ,  B01J2219/00822 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/0086 ,  B01J2219/00891 ,  B01L3/502776 ,  B01L2200/0636 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0887 ,  B01L2400/0487 ,  B65G51/00 ,  G01N15/1404 ,  G01N15/1484 ,  G01N2015/1409

摘要： A microfabricated sheath flow structure for producing a sheath flow includes a primary sheath flow channel for conveying a sheath fluid, a sample inlet for injecting a sample into the sheath fluid in the primary sheath flow channel, a primary focusing region for focusing the sample within the sheath fluid and a secondary focusing region for providing additional focusing of the sample within the sheath fluid. The secondary focusing region may be formed by a flow channel intersecting the primary sheath flow channel to inject additional sheath fluid into the primary sheath flow channel from a selected direction. A sheath flow system may comprise a plurality of sheath flow structures operating in parallel on a microfluidic chip.

摘要翻译： 用于产生鞘流的微制造鞘流结构包括用于输送鞘流体的主鞘流动通道，用于将样品注入到主鞘流动通道中的鞘液中的样本入口，用于将样本聚焦在 鞘液和辅助聚焦区，用于提供样品在鞘液内的附加聚焦。 次聚焦区域可以由与主鞘流动通道相交的流动通道形成，以将额外的鞘流体从选定方向注入主鞘流动通道。 鞘流系统可以包括在微流体芯片上平行操作的多个鞘流结构。

公开(公告)号：EP1581350A2

公开(公告)日：2005-10-05

申请号：EP03752432.9

申请日：2003-09-16

申请人： Cytonome, Inc.

发明人： GILBERT, John, R. ,  BÖHM, Sebastian ,  DESHPANDE, Manish

IPC分类号： B07C5/00

CPC分类号： G01N15/1459 ,  G01N2015/1477 ,  G01N2015/149 ,  G01N2015/1497

摘要： A method and apparatus for sorting particles moving through a closed channel system of capillary size comprises a bubble valve for selectively generating a pressure pulse to separate a particle having a predetermined characteristic from a stream of particles. The particle sorting system may further include a buffer for absorbing the pressure pulse. The particle sorting system may include a plurality of closely coupled sorting modules which are combined to further increase the sorting rate. The particle sorting system may comprise a multi-stage sorting device for serially sorting streams of particles, in order to decrease the error rate.

公开(公告)号：EP1499440A1

公开(公告)日：2005-01-26

申请号：EP03721599.3

申请日：2003-04-09

申请人： Cytonome, Inc.

发明人： GILBERT, John, R. ,  TRIKHA, Jaishree

IPC分类号： B01L3/00

CPC分类号： C40B50/08 ,  B01L3/0244 ,  B01L3/0248 ,  B01L3/0255 ,  B01L3/06 ,  B01L3/5088 ,  B01L2200/142 ,  B01L2200/143 ,  B01L2200/147 ,  B01L2300/1822 ,  B01L2400/0439 ,  C40B40/10 ,  G01N2035/1037 ,  Y10T436/25 ,  Y10T436/2575

摘要： A two-pin liquid handling system (10) for crystallizing biomolecules comprises a pair of interacting pins (11, 12) for holding a droplet of liquid therebetween. Each pin (11, 12) includes a tip (11a, 12a) spaced predetermined distance from the other pin to define a sample acquisition region (14). The two-pin liquid handling system (10) includes a temperature control element (200) for controlling the temperature of the sample acquisition region (14) to cause transpiration of water to and from a droplet (18) of biomolecule solution to promote crystallization of the biomolecule.