Porcine GRF receptor
    1.
    发明公开
    Porcine GRF receptor 失效
    Rekombinanter Schweine-GRF-Rezeptor。

    公开(公告)号:EP0604224A1

    公开(公告)日:1994-06-29

    申请号:EP93310446.5

    申请日:1993-12-22

    CPC分类号: C07K14/71 A01K2217/05

    摘要: The instant invention provides the porcine GRF receptor protein. The invention also provides DNA compounds encoding the pGRF receptor, recombinant DNA cloning and expression vectors comprising said DNA sequence, and recombinant host cells comprising said vectors. The invention also provides methods of making the pGRF receptor in recombinant and solid phase systems. The invention also provides methods of using the pGRF receptor in assay systems. The invention also provides methods of using said DNA sequences as probes to isolate and characterize GRF receptors and corresponding DNA sequences from other species.

    摘要翻译: 本发明提供了猪GRF受体蛋白。 本发明还提供编码pGRF受体的DNA化合物,包含所述DNA序列的重组DNA克隆和表达载体,以及包含所述载体的重组宿主细胞。 本发明还提供了在重组和固相体系中制备pGRF受体的方法。 本发明还提供了在测定系统中使用pGRF受体的方法。 本发明还提供了使用所述DNA序列作为探针以分离和表征GRF受体和来自其他物种的相应DNA序列的方法。

    Vectors for expressing bovine growth hormone derivatives
    2.
    发明公开
    Vectors for expressing bovine growth hormone derivatives 失效
    表达方式是Rinderwachstumshormonderivate。

    公开(公告)号:EP0159123A2

    公开(公告)日:1985-10-23

    申请号:EP85301468.6

    申请日:1985-03-04

    IPC分类号: C12N15/62 C12N15/69 C07K15/00

    摘要: The present invention relates to novel selectable and autonomously replicating recombinant DNA expression vectors which code for bovine growth hormone derivatives. The invention further comprises novel transformants of the aforementioned vectors, a method of making proteinaceous granules and a method of use.

    摘要翻译: (1)可选择和自主复制的重组DNA表达载体包含(a)失控复制子时是新的; 和(b)编码生物活性牛生长激素(BGH)衍生物的基因的阅读框中的转录和翻译活化序列。 该基因是6个确定的核苷酸三联体序列之一,每个序列携带编码与定义的编码转录停止信号的DNA序列的BGH氨基酸9-191的DNA序列。 (2)BGH衍生。 由载体编码的是式(I) - (II),并且是新的。 Met-Phe-Pro-Ala-Met-Ala-R2(I)Met-Asp-Asp-Lys-R3(II)Met-Asp-R3(III)(R2 = BGH的天然氨基酸序列, N末端; R3 =来自N-末端(第一)苯丙氨酸的BGH的天然氨基酸序列)。 (3)用如上定义的载体转化的宿主细胞是新的。 (4)质粒pCZ103也是新的。 (5)产品 的细胞内高度均匀的蛋白质颗粒物质包含(a)用可选择和自主复制的重组DNA表达载体转化感受态细胞,所述重组DNA表达载体包含失控复制子以及转录和翻译活化序列,所述转录和翻译活化序列在编码 功能性多肽(IV)。 该序列包含紧邻(IV)的羧基末端的核苷酸三联体的紧邻和下游的平移停止信号; 和(b)在适合于基因表达和激活或诱导失控复制子的条件下培养转化的细胞。 -

    Process for de-cyanoethylating blocked nucleotides
    3.
    发明公开
    Process for de-cyanoethylating blocked nucleotides 失效
    Verfahren zur De-cyanoethylierung von blockierten Nucleotiden。

    公开(公告)号:EP0075392A1

    公开(公告)日:1983-03-30

    申请号:EP82304434.2

    申请日:1982-08-23

    IPC分类号: C07H21/00

    CPC分类号: C07H21/00

    摘要: A process for selectively removing a β-cyanoethyl blocking group from the phosphate moiety of a nucleotide or polynucleotide, which comprises treating said nucleotide or polynucleotide with a primary amine having from 3 to 5 carbon atoms or diethylamine.

    摘要翻译: 选择性地从核苷酸或多核苷酸的磷酸酯部分除去β-氰基乙基封闭基团的方法,其包括用具有3至5个碳原子的伯胺或二乙胺处理所述核苷酸或多核苷酸。

    Obesity gene product
    5.
    发明公开
    Obesity gene product 失效
    肥胖基因产物

    公开(公告)号:EP0743321A3

    公开(公告)日:1997-01-15

    申请号:EP96303478.0

    申请日:1996-05-16

    IPC分类号: C07K14/00

    CPC分类号: C07K14/5759 A61K38/00

    摘要: This invention is in the general field of molecular biology as applied to pharmaceutical or animal health research and development. The invention includes proteins, DNA compounds, vectors, and methods useful for expressing proteins that are regulate the body's volume of adipose tissue.

    摘要翻译: 本发明属于应用于制药或动物健康研究和开发的分子生物学领域。 本发明包括蛋白质,DNA化合物,载体和用于表达调节身体脂肪组织体积的蛋白质的方法。

    Improved bacteriophage lambda pL promoters
    6.
    发明公开
    Improved bacteriophage lambda pL promoters 失效
    动物园Bakteriophage-lambda-pL-Promotoren。

    公开(公告)号:EP0471514A2

    公开(公告)日:1992-02-19

    申请号:EP91307325.0

    申请日:1991-08-09

    IPC分类号: C12N15/73

    CPC分类号: C07K14/61 C12N15/73

    摘要: The present invention concerns novel DNA compounds which function as transcriptional activating sequences. Recombinant DNA expression vectors which contain the transcriptional activating sequences and host cells transformed with these expression vectors are also provided. When properly positioned in an expression vector, the novel transcriptional activating sequences enable regulatable expression of an operably linked gene and enhance the structural stability of the expression vector.

    摘要翻译: 本发明涉及用作转录激活序列的新型DNA化合物。 还提供了含有转录激活序列和用这些表达载体转化的宿主细胞的重组DNA表达载体。 当正确位于表达载体中时,新的转录激活序列使得可操纵连接的基因的可调节表达增强了表达载体的结构稳定性。

    Precursor forms of porcine growth hormone releasing factor and related DNA compounds
    8.
    发明公开
    Precursor forms of porcine growth hormone releasing factor and related DNA compounds 失效
    Vorläuferformendes Schweinewachstumshormonfreisetzungsfaktors und verwandte DNS-Verbindungen。

    公开(公告)号:EP0459747A1

    公开(公告)日:1991-12-04

    申请号:EP91304796.5

    申请日:1991-05-28

    IPC分类号: C12N15/16 C07K7/10 A61K37/43

    CPC分类号: C07K14/60 A61K38/00

    摘要: The present invention provides polypeptide compounds which are two forms of the porcine growth hormone releasing factor (pGRF) precursor and the pGRF leader peptide, and a new form of mature pGRF. The invention also provides DNA compounds encoding these polypeptide compounds and mature pGRF, pharmaceutical compositions and methods for the inducement of growth hormone release.

    摘要翻译: 本发明提供了多种化合物,其是猪生长激素释放因子(pGRF)前体和pGRF前导肽的两种形式,以及新形式的成熟pGRF。 本发明还提供了编码这些多肽化合物和成熟pGRF的DNA化合物,用于诱导生长激素释放的药物组合物和方法。

    Growth hormone releasing factor analogs
    9.
    发明公开
    Growth hormone releasing factor analogs 失效
    Wachstumshormon-Releasingfaktor-类比。

    公开(公告)号:EP0454367A2

    公开(公告)日:1991-10-30

    申请号:EP91303507.7

    申请日:1991-04-19

    IPC分类号: C07K7/10

    CPC分类号: C07K14/60 A61K38/00

    摘要: The present invention provides novel analogs of growth hormone releasing factor (GRF). Chemically modified versions of these molecules are also provided as are DNA compounds encoding GRF analogs. Some of the polypeptide compounds can be obtained in large yields by production of the compounds in E . coli . The invention also provides pharmaceutical compositions and methods for the inducement of growth hormone release.

    摘要翻译: 本发明提供了生长激素释放因子(GRF)的新型类似物。 还提供了这些分子的化学修饰形式,就像编码GRF类似物的DNA化合物一样。 通过在大肠杆菌中生产化合物可以大量获得一些多肽化合物。 本发明还提供用于诱导生长激素释放的药物组合物和方法。