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公开(公告)号：EP1974057A2
公开(公告)日：2008-10-01
申请号：EP06847988.0
申请日：2006-12-20
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q2525/186 , C12Q2521/525 , C12Q2521/319 , C12Q2521/301
摘要： The invention provides a method of determining the nucleotide sequence of a target nucleic acid using a reversibly terminating nucleotide that is modified at the 2' position.
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公开(公告)号：EP0543942B1
公开(公告)日：2001-01-24
申请号：EP91917050.6
申请日：1991-08-06
CPC分类号： C12Q1/686 , C07H21/00 , C12Q1/6818 , C12Q1/6823 , C12Q1/6827 , C12Q1/703 , Y10S435/805 , Y10S435/81 , Y10S436/815 , C12Q2521/101 , C12Q2565/1015 , C12Q2531/113 , C12Q2521/319 , C12Q2535/131 , C12Q2521/325 , C12Q2537/157 , C12Q2561/101 , C12Q2525/186 , C12Q2525/131
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公开(公告)号：EP0550687B1
公开(公告)日：1999-06-09
申请号：EP91919358.1
申请日：1991-09-30
CPC分类号： C12N9/1252
摘要： The present invention relates to thermostable DNA polymerases which exhibit a different level of 5' to 3' exonuclease activity than their respective native polymerases. Particular conserved amino acid domains in thermostable DNA polymerases are mutated or deleted to alter the 5' to 3' exonuclease activity of the polymerases. The present invention also relates to means for isolating and producing such altered polymerases.
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4.发明授权
公开(公告)号：EP0437459B1
公开(公告)日：1997-10-29
申请号：EP89910787.4
申请日：1989-09-19
IPC分类号： C12Q1/68
CPC分类号： C12Q1/701 , C12Q1/686 , C12Q1/6869 , Y10S435/81 , Y10S436/808 , C12Q2535/101
摘要： Dideoxynucleotide DNA sequencing methods can be dramatically improved by utilizing the DNA polymerase from Thermus aquaticus to catalyze the primer extension reactions.
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5.发明授权THE REDUCTION OF NON-SPECIFIC AMPLIFICATION DURING $i(IN VITRO) NUCLEIC ACID AMPLIFICATION USING MODIFIED NUCLEIC ACID BASES 失效非特异性扩增的A（体外）核酸扩增时的压下使用修改NUCLEIC BASES
公开(公告)号：EP0540693B1
公开(公告)日：1999-01-20
申请号：EP91916353.5
申请日：1991-07-23
CPC分类号： C12N9/1276 , C12Q1/6844 , C12Q2527/101 , C12Q2525/117 , C12Q2521/531
摘要： Improved methods for amplifying nucleic acids can reduce non-specific amplification and minimize the effects of contamination of nucleic acid amplification reaction assays due to amplified product from previous amplifications. The methods involve the introduction of unconventional nucleotide bases into the amplification reaction products and treating the products by enzymatic (e.g., glycosylases) and/or physical-chemical means to render the product incapable of acting as a template for subsequent amplifications.
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公开(公告)号：EP0395736B1
公开(公告)日：1996-03-20
申请号：EP89902422.8
申请日：1989-01-12
CPC分类号： C12P19/34 , C12N9/1252 , C12N9/1276 , C12N15/70
摘要： Recombinant DNA sequences encoding a thermostable DNA polymerase from Thermus aquaticus can be used to produce a recombinant protein with a molecular weight of about 86,000-95,000 daltons. The thermostable recombinant enzyme can be used in a temperature-cycling chain reaction wherein at least one nucleic acid sequence is amplified in quantity from an existing sequence with the aid of selected primers and nucleotide triphosphates. The enzyme is preferably stored in a buffer containing non-ionic detergents that lends stability to the enzyme.
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7.发明公开RECOMBINANT EXPRESSION VECTORS AND PURIFICATION METHODS FOR $i(THERMUS THERMOPHILUS) DNA POLYMERASE 失效重组表达载体和清洗过程DNA聚合酶OFF嗜热菌。
公开(公告)号：EP0506825A1
公开(公告)日：1992-10-07
申请号：EP91902036.0
申请日：1990-12-21
CPC分类号： C12Q1/686 , C12N9/1252 , C12N9/1276
摘要： On peut utiliser des séquences d'ADN recombiné codant l'activité de polymérase I d'ADN de Thermus Thermophilus afin d'élaborer des vecteurs recombinés, ainsi que des cellules hôtes transformées destinées à la production de l'activité. La polymérase I d'ADN de T. thermophilus est une protéine de ~94 kDa, utile notamment dans la technique d'amplification d'ADN appelée réaction en chaîne de polymérase.
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公开(公告)号：EP0506889B1
公开(公告)日：1997-04-02
申请号：EP91904087.3
申请日：1990-12-21
CPC分类号： C12Q1/6876 , C12N9/1252 , C12N9/1276 , C12Q1/6844 , C12Q1/686 , C12Q2600/158 , C12Q2527/101 , C12Q2521/107
摘要： Methods are provided for the replication and amplification of RNA sequences by thermoactive DNA polymerases. Reverse transcription of RNA is catalyzed by, for example, 94 kDa Taq, 62 kDa Taq, nTth and recombinant Tth DNA polymerase. Reverse transcription is coupled to PCR amplification in a one enzyme procedure using a thermostable polymerase.
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公开(公告)号：EP1641943B1
公开(公告)日：2013-03-20
申请号：EP04756455.4
申请日：2004-06-29
发明人： GELFAND, David, H. , REICHERT, Fred, L. , BODEPUDI, Veeraiah , GUPTA, Amar , WILL, Stephen, G. , MYERS, Thomas, B.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , Y10S435/975 , C12Q2535/101 , C12Q2525/101 , C12Q2525/186
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公开(公告)号：EP0543942B2
公开(公告)日：2006-11-15
申请号：EP91917050.6
申请日：1991-08-06
CPC分类号： C12Q1/686 , C07H21/00 , C12Q1/6818 , C12Q1/6823 , C12Q1/6827 , C12Q1/703 , Y10S435/805 , Y10S435/81 , Y10S436/815 , C12Q2521/101 , C12Q2565/1015 , C12Q2531/113 , C12Q2521/319 , C12Q2535/131 , C12Q2521/325 , C12Q2537/157 , C12Q2561/101 , C12Q2525/186 , C12Q2525/131
摘要： A process of detecting a target nucleic acid using labeled oligonucleotides uses the 5' to 3' nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and release labeled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification assay.
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