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10 条记录 (耗时 0.016 秒)

    GENE THERAPY FOR TREATING OCULAR-RELATED DISORDERS
    1.
    发明公开
    GENE THERAPY FOR TREATING OCULAR-RELATED DISORDERS 审中-公开
    基因治疗眼睛投诉处理

    公开(公告)号：EP1253948A2

    公开(公告)日：2002-11-06

    申请号：EP01909022.4

    申请日：2001-02-09

    申请人： GENVEC, INC.

    发明人： KOVESDI, Imre ,  BROUGH, Douglas, E. ,  MCVEY, Duncan, L. ,  WEI, Lisa

    IPC分类号： A61K48/00

    CPC分类号： A61K48/0075 ,  A61K48/005 ,  C07K14/71 ,  C07K14/811 ,  C12N15/86 ,  C12N2710/10343

    摘要： The present invention is directed to a method of prophylactically or therapeutically treating an animal for at least one ocular-related disorder, e.g., ocular neovascularization or age-related macular degeneration. The method comprises contacting an ocular cell with an expression vector comprising a nucleic acid sequence encoding an inhibitor of angiogenesis and the same or different nucleic acid sequence encoding a neurotrophic agent. The method also can comprise contacting an ocular cell with different expression vectors, each comprising a nucleic acid sequence encoding an inhibitor of angiogenesis and/or a nucleic acid sequence encoding a neurotrophic agent. In addition, the present invention provides a viral vector comprising a nucleic acid sequence encoding pigment epithelium-derived factor (PEDF) or a therapeutic fragment thereof.

    METHODS AND VECTORS FOR SITE-SPECIFIC RECOMBINATION
    3.
    发明公开
    METHODS AND VECTORS FOR SITE-SPECIFIC RECOMBINATION 失效
    方法和载体为寻找重组

    公开(公告)号：EP0850312A1

    公开(公告)日：1998-07-01

    申请号：EP96929879.0

    申请日：1996-08-27

    申请人： GENVEC, INC.

    发明人： MCVEY, Duncan, L. ,  KOVESDI, Imre

    IPC分类号： C12N15 ,  C12N9

    CPC分类号： C12Y302/01031 ,  C12N9/2434 ,  C12N15/90 ,  C12N2800/108 ,  C12N2800/30 ,  C12N2840/20 ,  C12N2840/44

    摘要： The present invention provides methods for site-specific recombination in a cell, as well as vectors which can be employed in such methods. The methods and vectors of the present invention can be used to obtain persistent gene expression in a cell and to modulate gene expression. One preferred method according to the invention comprises contacting a cell with a vector comprising an origin of replication functional in mammalian cells located between first and second recombining sites located in parallel. Another preferred method comprises, in part, contacting a cell with a vector comprising first and second recombining sites in antiparallel orientations such that the vector is internalized by the cell. In both methods, the cell is further provided with a site-specific recombinase that effects recombination between the first and second recombining sites of the vector.

    ANTIGENIC COMPLEXES AND METHODS
    4.
    发明公开
    ANTIGENIC COMPLEXES AND METHODS 审中-公开
    抗原复合体和方法

    公开(公告)号：EP1259256A1

    公开(公告)日：2002-11-27

    申请号：EP01908903.6

    申请日：2001-02-07

    申请人： GENVEC, INC.

    发明人： ROELVINK, Petrus, W. ,  KOVESDI, Imre ,  BRUDER, Joseph, T. ,  BROUGH, Douglas, E. ,  MCVEY, Duncan, L. ,  WICKHAM, Thomas, J.

    IPC分类号： A61K39/00 ,  C12N7/01 ,  C12N15/861 ,  G01N33/53

    CPC分类号： C40B40/02 ,  A61K47/6901 ,  A61K2039/5256 ,  A61K2039/5258 ,  C12N15/1037 ,  C12N2799/022

    摘要： The present invention provides a complex that includes a virion having a ligand that recognizes an epitope present on an immune effector cell surface and at least a first nucleic acid encoding a first non-native antigen. The invention also provides a library including a plurality of such complexes, in which antigens of at least two of the plurality are different. Using such reagents, the invention provides a method of precipitating an immune response within an effector cell, wherein such a complex is delivered to the cell under conditions sufficient for the cell to mount an immune response to the antigen. When applied in vivo, the method can serve to immunize an animal from the pathogen. Moreover, using a library including a plurality of complexes, which contains at least one test antigen, the invention provides a method of assessing the antigenicity of the test antigen.

    DUAL SELECTION CASSETTE AND PLASMIDS CONTAINING SAME
    8.
    发明授权
    DUAL SELECTION CASSETTE AND PLASMIDS CONTAINING SAME 有权
    双选择盒和含有PLASMIDE

    公开(公告)号：EP1015620B1

    公开(公告)日：2005-08-31

    申请号：EP98950669.6

    申请日：1998-09-23

    申请人： GenVec, Inc.

    发明人： MCVEY, Duncan, L. ,  BROUGH, Douglas, E. ,  KOVESDI, Imre

    IPC分类号： C12N15/86

    CPC分类号： C12N15/86 ,  C12N15/65 ,  C12N2710/10343 ,  C12N2800/30 ,  C12N2830/001 ,  C12N2830/15 ,  C12N2830/55

    摘要： The present invention provides a dual selection cassette (DSC) comprising first and second DNA segments having homology to a eukaryotic viral vector, positive and negative selection genes, each operably linked to their own promoter, and one or more unique restriction enzyme sites (URES) or site-directed homologous recombination sites. The present invention also provides a plasmid, pN/P, comprising an independent positive selection marker gene, an origine of replication, and a dual selection cassette. The dual selection cassette and pN/P plasmid can be used to produce eukaryotic gene transfer vectors without requiring temporally-linked double recombination events or the use of specialized bacterial strains that allow the replication of plasmids comprising defective origins of replication. This method usefully increases the ratio of desired to undesired plasmid and vector constructs. Additionally, this invention provides a method for the creation of eukaryotic viral vector libraries.

    METHODS OF PREPARING AND USING A VIRAL VECTOR LIBRARY
    9.
    发明公开
    METHODS OF PREPARING AND USING A VIRAL VECTOR LIBRARY 审中-公开
    工艺编制和病毒载体的文库

    公开(公告)号：EP1254212A2

    公开(公告)日：2002-11-06

    申请号：EP01907171.1

    申请日：2001-02-09

    申请人： GENVEC, INC.

    发明人： KOVESDI, Imre ,  MCVEY, Duncan, L. ,  WICKHAM, Thomas, J. ,  BRUDER, Joseph, T. ,  BROUGH, Douglas, E.

    IPC分类号： C12N5/10

    CPC分类号： C12N15/1093 ,  A61K48/00 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2710/10345 ,  C12N2810/40

    摘要： The present invention provides a library of viral vectors, wherein each member comprises a first heterologous DNA encoding a first gene product and a second heterologous DNA encoding a second gene product. The first heterologous DNA is common to each member of the library, while the second heterologous DNA varies between members of the library. The present invention additionally provides a method of constructing a library of viral vectors. The method comprises carrying out homologous recombination between a first DNA molecule and a second DNA molecule to form a pool of intermediate viral vector genomes. One or more linear third DNA molecules are ligated into the pool of intermediate viral geneomes to produce a library of viral vector genomes. Alternatively, homologous recombination between linear DNA molecules and recipient DNA molecules produces a library of viral vector genomes. The library of viral vector genomes is converted into a library of viral vectors.

    PLASMIDS FOR CONSTRUCTION OF EUKARYOTIC VIRAL VECTORS
    10.
    发明公开
    PLASMIDS FOR CONSTRUCTION OF EUKARYOTIC VIRAL VECTORS 有权
    双选择盒和含有PLASMIDE

    公开(公告)号：EP1015620A1

    公开(公告)日：2000-07-05

    申请号：EP98950669.6

    申请日：1998-09-23

    申请人： GENVEC, INC.

    发明人： MCVEY, Duncan, L. ,  BROUGH, Douglas, E. ,  KOVESDI, Imre

    IPC分类号： C12N15/86

    CPC分类号： C12N15/86 ,  C12N15/65 ,  C12N2710/10343 ,  C12N2800/30 ,  C12N2830/001 ,  C12N2830/15 ,  C12N2830/55

    摘要： The present invention provides a dual selection cassette (DSC) comprising first and second DNA segments having homology to a eukaryotic viral vector, positive and negative selection genes, each operably linked to their own promoter, and one or more unique restriction enzyme sites (URES) or site-directed homologous recombination sites. The present invention also provides a plasmid, pN/P, comprising an independent positive selection marker gene, an origine of replication, and a dual selection cassette. The dual selection cassette and pN/P plasmid can be used to produce eukaryotic gene transfer vectors without requiring temporally-linked double recombination events or the use of specialized bacterial strains that allow the replication of plasmids comprising defective origins of replication. This method usefully increases the ratio of desired to undesired plasmid and vector constructs. Additionally, this invention provides a method for the creation of eukaryotic viral vector libraries.