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公开(公告)号：EP3734279A1
公开(公告)日：2020-11-04
申请号：EP20181620.4
申请日：2014-03-11
发明人： REY, Diego Ariel , ROY, Shaunak , TEIXEIRA, Leonardo M. , GRISWOLD, Ryan C. , MATTHEWS, Damian S. , OLSON, Kenneth G. , RICHARDSON, Bruce J. , STELLMACHER, Rick V. , YEE, Victor H.
IPC分类号： G01N33/53 , G01N33/542 , C12P19/34 , C12Q1/04 , C12Q1/6897 , C12Q1/70 , G01N35/00
摘要： Systems and methods for detecting and/or identifying target cells (e.g., bacteria) using engineered transduction particles are described herein. In some embodiments, a method includes mixing a quantity of transduction particles within a sample. The transduction particles are associated with a targe t cell. The tran sduction particles are non -replicative, and are engineered to include a nucleic acid molecule formulated to cause the target cell to produce a series of re porter molecules. The s ample and the transduction particles are maintained to express the series of the reporter molecules when target cell is present in the sample. A signal associated with a quantity of the reporter molecules is rece ived. In some embodiments, a magnitude of the signal is in dependent from a quantity of the transduction particle above a predetermined quantity.
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2.发明公开SYSTEMS AND METHODS FOR DETECTION OF CELLS USING ENGINEERED TRANSDUCTION PARTICLES 审中-公开系统VERFAHREN ZUR DETEKTION VON ZELLEN MITHILFE MANIPULERERTER TRANSDUKTIONSPARTIKEL
公开(公告)号：EP2972354A1
公开(公告)日：2016-01-20
申请号：EP14779477.0
申请日：2014-03-11
发明人： REY, Diego Ariel , ROY, Shaunak , TEIXEIRA, Leonardo M. , GRISWOLD, Ryan C. , MATTHEWS, Damian S. , OLSON, Kenneth G. , RICHARDSON, Bruce J. , STELLMACHER, Rick V. , YEE, Victor H.
IPC分类号： G01N33/53 , G01N33/542 , C12P19/34
CPC分类号： C12Q1/6897 , B01L3/502 , B01L3/50825 , B01L3/52 , B01L3/523 , B01L3/54 , B01L3/545 , B01L7/00 , B01L2200/028 , B01L2200/16 , B01L2300/021 , B01L2300/042 , B01L2300/047 , B01L2300/049 , B01L2300/0672 , B01L2400/0478 , B01L2400/06 , B01L2400/0683 , C12Q1/02 , C12Q1/04 , C12Q1/06 , C12Q1/10 , C12Q1/14 , C12Q1/66 , G01J1/00 , G01N21/01 , G01N21/03 , G01N21/13 , G01N21/76 , G01N21/763 , G01N2035/00346 , G01N2035/0405 , G01N2333/195
摘要： Systems and methods for detecting and/or identifying target cells (e.g., bacteria) using engineered transduction particles are described herein. In some embodiments, a method includes mixing a quantity of transduction particles within a sample. The transduction particles are associated with a target cell. The transduction particles are non-replicative, and are engineered to include a nucleic acid molecule formulated to cause the target cell to produce a series of reporter molecules. The sample and the transduction particles are maintained to express the series of the reporter molecules when target cell is present in the sample. A signal associated with a quantity of the reporter molecules is received. In some embodiments, a magnitude of the signal is independent from a quantity of the transduction particle above a predetermined quantity.
摘要翻译： 本文描述了使用工程改造的转导颗粒检测和/或鉴定靶细胞（例如细菌）的系统和方法。 在一些实施方案中，一种方法包括将一定量的转导颗粒混合在样品内。 转导颗粒与靶细胞相关联。 转导颗粒是非复制型的，并被工程化以包括配制成使靶细胞产生一系列报道分子的核酸分子。 当样品中存在靶细胞时，保持样品和转导颗粒以表达一系列报告分子。 接收与一定量的报道分子相关的信号。 在一些实施例中，信号的幅度与高于预定量的转导颗粒的数量无关。
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公开(公告)号：EP3401680B1
公开(公告)日：2020-09-30
申请号：EP18179228.4
申请日：2014-03-11
发明人： REY, Diego Ariel , ROY, Shaunak , TEIXEIRA, Leonardo M. , GRISWOLD, Ryan C. , MATTHEWS, Damian S. , OLSON, Kenneth G. , RICHARDSON, Bruce J. , STELLMACHER, Rick V. , YEE, Victor H.
IPC分类号： G01N33/53 , G01N33/542 , C12P19/34
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公开(公告)号：EP2972354B1
公开(公告)日：2018-10-03
申请号：EP14779477.0
申请日：2014-03-11
发明人： REY, Diego Ariel , ROY, Shaunak , TEIXEIRA, Leonardo M. , GRISWOLD, Ryan C. , MATTHEWS, Damian S. , OLSON, Kenneth G. , RICHARDSON, Bruce J. , STELLMACHER, Rick V. , YEE, Victor H.
IPC分类号： G01N33/53 , G01N33/542 , C12P19/34
CPC分类号： C12Q1/6897 , B01L3/502 , B01L3/50825 , B01L3/52 , B01L3/523 , B01L3/54 , B01L3/545 , B01L7/00 , B01L2200/028 , B01L2200/16 , B01L2300/021 , B01L2300/042 , B01L2300/047 , B01L2300/049 , B01L2300/0672 , B01L2400/0478 , B01L2400/06 , B01L2400/0683 , C12Q1/02 , C12Q1/04 , C12Q1/06 , C12Q1/10 , C12Q1/14 , C12Q1/66 , G01J1/00 , G01N21/01 , G01N21/03 , G01N21/13 , G01N21/76 , G01N21/763 , G01N2035/00346 , G01N2035/0405 , G01N2333/195
摘要： Systems and methods for detecting and/or identifying target cells (e.g., bacteria) using engineered transduction particles are described herein. In some embodiments, a method includes mixing a quantity of transduction particles within a sample. The transduction particles are associated with a target cell. The transduction particles are non-replicative, and are engineered to include a nucleic acid molecule formulated to cause the target cell to produce a series of reporter molecules. The sample and the transduction particles are maintained to express the series of the reporter molecules when target cell is present in the sample. A signal associated with a quantity of the reporter molecules is received. In some embodiments, a magnitude of the signal is independent from a quantity of the transduction particle above a predetermined quantity.
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