公开(公告)号：EP2989113A2

公开(公告)日：2016-03-02

申请号：EP14788673.3

申请日：2014-04-25

申请人： Genomatica, Inc.

发明人： PHARKYA, Priti ,  BURGARD, Anthony P. ,  VAN DIEN, Stephen J. ,  OSTERHOUT, Robin E. ,  BURK, Mark J. ,  TRAWICK, John D. ,  KUCHINSKAS, Michael P. ,  STEER, Brian ,  ANDRAE, Stefan ,  SHAH, Amit

IPC分类号： C07H21/04 ,  C12P21/06 ,  C12P7/16 ,  C12N9/10 ,  C12N9/14 ,  C12N15/00

CPC分类号： C12P7/18 ,  C07C31/207 ,  C07C47/19 ,  C07C211/09 ,  C07D307/33 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/13 ,  C12N15/52 ,  C12P7/24 ,  C12P7/42 ,  C12P7/62 ,  C12P13/001 ,  C12P13/005 ,  C12P17/04 ,  C12P19/32 ,  C12Y208/03

摘要： The invention provides non-naturally occurring microbial organisms having a 4-hydroxybutyrate, gamma-butyrolactone, 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-CoA and/or putrescine pathway and being capable of producing 4-hydroxybutyrate, wherein the microbial organism comprises one or more genetic modifications. The invention additionally provides methods of producing 4-hydroxybutyrate, gamma-butyrolactone, 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-CoA and/or putrescine or related products using the microbial organisms.

摘要翻译： 本发明提供具有4-羟基丁酸酯，γ-丁内酯，1,4-丁二醇，4-羟基丁醛，4-羟基丁酰-CoA和/或腐胺途径并且能够产生4-羟基丁酸酯的非天然存在的微生物，其中 微生物生物体包含一种或多种遗传修饰。 本发明另外提供了使用微生物生产4-羟基丁酸酯，γ-丁内酯，1,4-丁二醇，4-羟基丁醛，4-羟基丁酰-CoA和/或腐胺或相关产物的方法。

公开(公告)号：EP4421181A2

公开(公告)日：2024-08-28

申请号：EP24162808.0

申请日：2015-09-18

申请人： Genomatica, Inc.

发明人： PHARKYA, Priti ,  BURGARD, Anthony P. ,  NUNEZ VAN NAME, Eric Roland

IPC分类号： C12P7/16

CPC分类号： C12Y401/02022 ,  C12N9/88 ,  C12P7/18 ,  C12P7/42 ,  C12N15/52 ,  C12N9/1205 ,  C12Y207/0104 ,  C12Y401/02009 ,  Y02E50/10 ,  Y02E50/30

摘要： The invention provides non-natural microbial organisms containing enzymatic pathways and/or metabolic modifications for enhancing carbon flux through acetyl-CoA, or oxaloacetate and acetyl-CoA. Embodiments of the invention include microbial organisms having a pathway to acetyl-CoA and oxaloacetate that includes phosphoketolase (a PK pathway). The organisms also have either (i) a genetic modification that enhances the activity of the non-phosphotransferase system (non-PTS) for sugar uptake, and/or (ii) a genetic modification(s) to the organism's electron transport chain (ETC) that enhances efficiency of ATP production, that enhances availability of reducing equivalents or both. The microbial organisms can optionally include (iii) a genetic modification that maintains, attenuates, or eliminates the activity of a phosphotransferase system (PTS) for sugar uptake. The enhanced carbon flux through acetyl-CoA and oxaloacetate can be used for production of a bioderived compound, and the microbial organisms can further include a pathway capable of producing the bioderived compound.

公开(公告)号：EP4273255A3

公开(公告)日：2024-01-03

申请号：EP23182826.0

申请日：2010-05-07

申请人： Genomatica, Inc.

发明人： BURK, Mark J. ,  BURGARD, Anthony P. ,  OSTERHOUT, Robin E. ,  PHARKYA, Priti

IPC分类号： C12N1/38 ,  C12N15/52 ,  C12P7/40 ,  C12P7/44 ,  C12P13/02 ,  C12P17/10 ,  C12P13/00 ,  C12N9/10

摘要： The invention provides a non-naturally occurring microbial organism having a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The invention additionally provides a method for producing 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. The method can include culturing a 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid.

公开(公告)号：EP3450550A1

公开(公告)日：2019-03-06

申请号：EP18201495.1

申请日：2009-03-05

申请人： Genomatica, Inc.

发明人： SUN, Jun ,  PHARKYA, Priti ,  BURGARD, Anthony P.

IPC分类号： C12N9/02 ,  C12N9/04 ,  C12P7/02 ,  C12P7/04 ,  C12N15/52

摘要： The invention provides a non-naturally occurring microbial organism having a microbial organism having at least one exogenous gene insertion and/or one or more gene disruptions that confer production of primary alcohols. A method for producing long chain alcohols includes culturing these non-naturally occurring microbial organisms.

公开(公告)号：EP3792352A3

公开(公告)日：2021-06-16

申请号：EP20184529.4

申请日：2013-08-27

申请人： Genomatica, Inc.

发明人： BURGARD, Anthony P. ,  OSTERHOUT, Robin E. ,  VAN DIEN, Stephen J. ,  TRACEWELL, Cara Ann ,  PHARKYA, Priti ,  ANDRAE, Stefan

IPC分类号： C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C12N9/88 ,  C12N1/21 ,  C12P7/18

摘要： Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway (MMP) that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 1,4-butanediol (BDO). Also provided herein are methods for using such an organism to produce BDO.

公开(公告)号：EP3611254A1

公开(公告)日：2020-02-19

申请号：EP19186319.0

申请日：2010-05-07

申请人： Genomatica, Inc.

发明人： BURK, Mark J. ,  BURGARD, Anthony P. ,  OSTERHOUT, Robin E. ,  PHARKYA, Priti

IPC分类号： C12N1/38 ,  C12N15/52 ,  C12P7/40 ,  C12P7/44 ,  C12P13/02 ,  C12P17/10 ,  C12P13/00

摘要： The invention provides a non-naturally occurring microbial organism having a hexamethylenediamine pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the hexamethylenediamine pathway. The invention additionally provides a method for producing hexamethylenediamine. The method can include culturing a hexamethylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a hexamethylenediamine pathway enzyme in a sufficient amount to produce the hexamethylenediamine, under conditions and for a sufficient period of time to produce hexamethylenediamine.

公开(公告)号：EP2679676B1

公开(公告)日：2018-07-18

申请号：EP13001197.6

申请日：2008-08-06

申请人： Genomatica, Inc.

发明人： BURGARD, Anthony P. ,  VAN DIEN, Stephen J. ,  BURK, Mark J.

IPC分类号： C12N9/04 ,  C12P7/18

CPC分类号： C12P7/18 ,  B01D3/002 ,  C12N9/0006

摘要： The invention provides a non-naturally occurring microorganism comprising one or more gene disruptions, the one or more gene disruptions occurring in genes encoding an enzyme obligatory to coupling 1,4-butanediol production to growth of the microorganism when the gene disruption reduces an activity of the enzyme, whereby theone or more gene disruptions confers stable growth-coupled production of 1,4-butanediol onto the non-naturally occurring microorganism. The microorganism can further comprise a gene encoding an enzyme in a 1,4-butanediol (BDO) biosynthetic pathway. The invention additionally relates to methods of using microorganisms to produce BDO.

公开(公告)号：EP3135760A1

公开(公告)日：2017-03-01

申请号：EP16168278.6

申请日：2010-04-30

申请人： Genomatica, Inc.

发明人： BURGARD, Anthony P. ,  BURK, Mark J. ,  OSTERHOUT, Robin E. ,  PHARKYA, Priti

IPC分类号： C12N1/21 ,  C12P7/18 ,  C12N9/04

CPC分类号： C12P7/18 ,  C07C29/80 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/88 ,  C12N15/52 ,  C12Y101/01 ,  C12Y101/01157 ,  C12Y102/01 ,  C12Y203/01 ,  C12Y206/01 ,  C12Y401/01 ,  C12Y402/01 ,  C12Y403/01

摘要： A non-naturally occurring microbial organism includes a microbial organism having a 1,3-butanediol (1,3-BDO) pathway having at least one exogenous nucleic acid encoding a 1,3-BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO. The pathway includes an enzyme selected from a 2-amino-4-ketopentanoate (AKP) thiolase, an AKP dehydrogenase, a 2-amino-4-hydroxypentanoate aminotransferase, a 2-amino-4-hydroxypentanoate oxidoreductase (deaminating), a 2-oxo-4-hydroxypentanoate decarboxylase, a 3-hydroxybutyraldehyde reductase, an AKP aminotransferase, an AKP oxidoreductase (deaminating), a 2,4-dioxopentanoate decarboxylase, a 3-oxobutyraldehyde reductase (ketone reducing), a 3-oxobutyraldehyde reductase (aldehyde reducing), a 4-hydroxy-2-butanone reductase, an AKP decarboxylase, a 4-aminobutan-2-one aminotransferase, a 4-aminobutan-2- one oxidoreductase (deaminating), a 4-aminobutan-2-one ammonia-lyase, a butenone hydratase, an AKP ammonia-lyase, an acetylacrylate decarboxylase, an acetoacetyl-CoA reductase (CoA-dependent, aldehyde forming), an acetoacetyl-CoA reductase (CoA-dependent, alcohol forming), an acetoacetyl-CoA reductase (ketone reducing), a 3-hydroxybutyryl-CoA reductase (aldehyde forming), a 3-hydroxybutyryl-CoA reductase (alcohol forming), a 4-hydroxybutyryl- CoA dehydratase, and a crotonase. A method for producing 1,3-BDO, includes culturing such microbial organisms under conditions and for a sufficient period of time to produce 1,3-BDO.

摘要翻译： 非天然存在的微生物体包括具有1,3-丁二醇（1,3-BDO）途径的微生物生物，其具有至少一种编码1,3-BDO途径酶的外源核酸，其表达量足以产生1 ，3-BDO。 途径包括选自2-氨基-4-酮戊酸酯（AKP）硫解酶，AKP脱氢酶，2-氨基-4-羟基戊酸转氨酶，2-氨基-4-羟基戊酸酯氧化还原酶（脱氨基），2- 4-羟基戊酸脱羧酶，3-羟基丁醛还原酶，AKP氨基转移酶，AKP氧化还原酶（脱氨基），2,4-二氧代戊酸脱羧酶，3-氧代丁醛还原酶（还原酮），3-氧代丁醛还原酶 ），4-羟基-2-丁酮还原酶，AKP脱羧酶，4-氨基丁-2-酮氨基转移酶，4-氨基丁-2-酮氧化还原酶（脱氨基），4-氨基丁-2-酮氨裂解酶 乙酰乙酰辅酶A还原酶（CoA依赖性醛形成），乙酰乙酰辅酶A还原酶（CoA依赖性醇成型），乙酰乙酰辅酶A还原酶（酮 还原），3-羟基丁酰辅酶A还原酶（醛形成），3-羟基丁酸 yyl-CoA还原酶（醇形成），4-羟基丁酰辅酶A脱水酶和巴豆酸酶。 一种生产1,3-BDO的方法包括在条件和足够的时间内培养这些微生物生产1,3-BDO。

公开(公告)号：EP4273255A2

公开(公告)日：2023-11-08

申请号：EP23182826.0

申请日：2010-05-07

申请人： Genomatica, Inc.

发明人： BURK, Mark J. ,  BURGARD, Anthony P. ,  OSTERHOUT, Robin E. ,  PHARKYA, Priti

IPC分类号： C12P13/00

摘要： The invention provides a non-naturally occurring microbial organism having a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The invention additionally provides a method for producing 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. The method can include culturing a 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid.

公开(公告)号：EP3862421A1

公开(公告)日：2021-08-11

申请号：EP21150544.1

申请日：2013-12-16

申请人： Genomatica, Inc.

发明人： BURGARD, Anthony P. ,  OSTERHOUT, Robin E. ,  VAN DIEN, Stephen J. ,  TRACEWELL, Cara Ann ,  PHARKYA, Priti ,  ANDRAE, Stefan

IPC分类号： C12N1/21 ,  C08G63/78 ,  C12N9/04 ,  C12P7/24 ,  C12P7/44 ,  C08G69/08 ,  C08G69/16 ,  C08G73/02 ,  C12N15/52 ,  C12P13/00 ,  C12P17/10

摘要： Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as adipate, 6-aminocaproate, hexamethylenediamine or caprolactam. Also provided herein are methods for using such an organism to produce adipate, 6-aminocaproate, hexamethylenediamine or caprolactam.