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    PRODUCTION OF GLYCOPROTEINS WITH MAMMALIAN-LIKE N-GLYCANS IN FILAMENTOUS FUNGI
    3.
    发明公开
    PRODUCTION OF GLYCOPROTEINS WITH MAMMALIAN-LIKE N-GLYCANS IN FILAMENTOUS FUNGI 审中-公开
    哺乳动物丝状真菌中哺乳动物样N-糖苷生产糖蛋白

    公开(公告)号：EP3172333A1

    公开(公告)日：2017-05-31

    申请号：EP15738703.6

    申请日：2015-07-21

    申请人： Glykos Finland Oy

    发明人： NATUNEN, Jari ,  LEPPÄNEN, Anne ,  SALMINEN, Heidi ,  HILTUNEN, Jukka ,  KANERVA, Anne ,  HEISKANEN, Annamari ,  WESTERHOLM-PARVINEN, Ann ,  SCHMIDT, Georg ,  HUUSKONEN, Anne ,  MUSTALAHTI, Eero ,  LANDOWSKI, Christopher ,  SALOHEIMO, Markku ,  SAARINEN, Juhani ,  SOMMER, Benjamin Patrick ,  WAHL, Ramon ,  OSTERMEIER, Christian ,  HELK, Bernhard

    IPC分类号： C12P21/00 ,  C12N9/10 ,  C12N9/24

    摘要： The present disclosure relates to compositions and methods useful for the production of recombinant glycoproteins in filamentous fungal cells, such as Trichoderma cells, wherein at least 90% (mol %), preferably at least 95% of the total neutral N-glycans of said produced recombinant glycoprotein are mammalian-like N-glycans. More specifically, the invention provides a filamentous fungal cell comprising i. one or more mutations that reduces or eliminates one or more endogenous protease activity compared to a parental filamentous fungal cell which does not have said mutation(s); ii.a polynucleotide encoding a heterologous catalytic subunit of oligosaccharyl transferase; iii. a recombinant polynucleotide for increasing α1, 2 mannosidase activity;and, iv. a recombinant polynucleotide encoding said heterologous glycoprotein.

    摘要翻译： 本公开涉及用于在丝状真菌细胞例如木霉属细胞中产生重组糖蛋白的组合物和方法，其中所述产生的全部中性N-聚糖的至少90％（mol％），优选至少95％ 重组糖蛋白是哺乳动物样N-聚糖。 更具体地说，本发明提供了一种丝状真菌细胞，其包含i。 与不具有所述突变的亲本丝状真菌细胞相比，减少或消除一种或多种内源性蛋白酶活性的一种或多种突变; ii。编码寡糖基转移酶的异源催化亚基的多核苷酸; III。 用于增加α1,2甘露糖苷酶活性的重组多核苷酸;和iv。 编码所述异源糖蛋白的重组多核苷酸。

    PRODUCTION OF GLYCOPROTEINS HAVING INCREASED N-GLYCOSYLATION SITE OCCUPANCY
    5.
    发明公开
    PRODUCTION OF GLYCOPROTEINS HAVING INCREASED N-GLYCOSYLATION SITE OCCUPANCY 审中-公开
    枸杞子

    公开(公告)号：EP3019621A1

    公开(公告)日：2016-05-18

    申请号：EP14738496.0

    申请日：2014-07-10

    申请人： Novartis AG ,  Glykos Finland Oy

    发明人： NATUNEN, Jari ,  LANDOWSKI, Christopher ,  SALOHEIMO, Markku ,  OSTERMEIER, Christian ,  SOMMER, Benjamin Patrick ,  WAHL, Ramon

    IPC分类号： C12P21/00 ,  C12N9/10

    CPC分类号： C12P21/005 ,  C07K16/00 ,  C12N9/1051 ,  C12N9/1081 ,  C12N15/80 ,  C12Y204/01

    摘要： The present disclosure relates to compositions and methods useful for the production of heterologous proteins with increased N-glycosylation site occupancy in filamentous fungal cells, such as Trichoderma cells. More specifically, the invention provides a filamentous fungal cell comprising i. one or more mutation that reduces or eliminates one or more endogenous protease activity compared to a parental filamentous fungal cell which does not have said mutation(s), ii. a polynucleotide encoding a heterologous catalytic subunit of oligosaccharyl transferase, and iii. a polynucleotide encoding a heterologous glycoprotein, wherein said catalytic subunit of oligosaccharyl transferase is selected from Leishmania oligosaccharyl transferase catalytic subunits.

    摘要翻译： 本公开内容涉及用于生产丝状真菌细胞如木霉属细胞中具有增加的N-糖基化位点占据的异源蛋白质的组合物和方法。 更具体地，本发明提供丝状真菌细胞，其包含i。 一种或多种与不具有所述突变的亲本丝状真菌细胞相比减少或消除一种或多种内源蛋白酶活性的突变，ii。 编码寡糖基转移酶的异源催化亚基的多核苷酸，和iii。 编码异源糖蛋白的多核苷酸，其中所述寡糖基转移酶的催化亚单位选自利什曼原虫寡糖基转移酶催化亚基。

    O-MANNOSYLTRANSFERASE DEFICIENT FILAMENTOUS FUNGAL CELLS AND METHODS OF USE THEREOF
    6.
    发明公开
    O-MANNOSYLTRANSFERASE DEFICIENT FILAMENTOUS FUNGAL CELLS AND METHODS OF USE THEREOF 审中-公开
    O-MANNOSYLTRANSFERASEDEFIZIENTEFILAMENTÖSEPILZZELLEN UND VERFAHREN ZUR VERWENDUNG DAVON

    公开(公告)号：EP3016970A1

    公开(公告)日：2016-05-11

    申请号：EP14734515.1

    申请日：2014-07-03

    申请人： Novartis AG ,  Glykos Finland Oy

    发明人： NATUNEN, Jari ,  HILTUNEN, Jukka ,  HUUSKONEN, Anne ,  SALOHEIMO, Markku ,  OSTERMEIER, Christian ,  SOMMER, Benjamin Patrick ,  WAHL, Ramon

    IPC分类号： C07K14/37 ,  C07K16/00

    CPC分类号： C12N9/1051 ,  C07K14/37 ,  C07K16/00 ,  C07K16/18 ,  C07K2317/41 ,  C12Y204/01109

    摘要： The present disclosure relates to compositions and methods useful for the production of heterologous proteins with reduced O-mannosylation in filamentous fungal cells, such as Trichoderma cells. More specifically, the invention provides a PMT-deficient filamentous fungal cell comprising a) at least a first mutation that reduces an endogenous protease activity compared to a parental filamentous fungal cell which does not have said first mutation, and, b) at least a second mutation in a PMT gene that reduces endogenous O-mannosyltransferase activity compared to a parental filamentous fungal cell which does not have said second mutation, wherein said filamentous fungal cell is selected from the group consisting of Trichoderma, Neurospora, Myceliophthora or Chrysosporium cell.

    摘要翻译： 本公开涉及用于生产丝状真菌细胞如木霉属细胞中具有降低的O-甘露糖基化的异源蛋白质的组合物和方法。 更具体地，本发明提供了一种缺乏PMT的丝状真菌细胞，其包含a）至少一种与不具有所述第一突变的亲本丝状真菌细胞相比降低内源蛋白酶活性的第一突变，和b）至少第二突变 与不具有第二突变的亲本丝状真菌细胞相比，降低内源性O-甘露糖基转移酶活性的PMT基因中的突变，其中所述丝状真菌细胞选自木霉属，孢子孢子虫，丝霉菌属或孢子孢子菌属细菌。