公开(公告)号：EP4400589A1

公开(公告)日：2024-07-17

申请号：EP22867379.4

申请日：2022-09-07

申请人： Godo Shusei Co., Ltd.

发明人： ODAKA, Rei ,  SANO, Ryoko ,  IWASAKI, Takumi ,  BABA, Masahiro ,  SABURI, Wataru ,  MORI, Haruhide

IPC分类号： C12N15/56 ,  A23C7/04 ,  A23C9/12 ,  A23C21/02 ,  A23C23/00 ,  A23L33/00 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/38 ,  C12N15/63

CPC分类号： A23C7/04 ,  A23C9/12 ,  A23C21/02 ,  A23C23/00 ,  A23L33/00 ,  C12N5/10 ,  C12N9/24 ,  C12N9/2468 ,  C12N15/63 ,  C12N15/80 ,  C12N15/81 ,  C12N15/74

摘要： Provided is a lactase preparation having an excellent lactose decomposing action in milk. A lactase according to an aspect of the present invention has, as lactase activity, any one of the following activities (1) to (6) or a combination of two or more thereof:
(1) when the lactase is added to cow's milk, an added amount of protein in the lactase at which a residual lactose concentration reaches 0.1% or less after 16 hours at 10°C is 15.1 mg/L or less;
(2) when the lactase is added to cow's milk, the added amount of protein in the lactase at which the residual lactose concentration reaches 0.01% or less after 16 hours at 10°C is 30.2 mg/L or less;
(3) when the lactase is added to cow's milk, the added amount of protein in the lactase at which the residual lactose concentration reaches 0.1% or less after 24 hours at 10°C is 10.1 mg/L or less;
(4) when the lactase is added to cow's milk, the added amount of protein in the lactase at which the residual lactose concentration reaches 0.01% or less after 24 hours at 10°C is 15.1 mg/L or less;
(5) when the lactase is added to cow's milk in an amount that makes a final concentration 1.4 LYU/ml, the residual lactose concentration reaches 0.1% or less after 24 hours at 10°C; and
(6) when the lactase is added to cow's milk in the amount that makes a final concentration 2.1 LYU/ml, the residual lactose concentration reaches 0.01% or less after 24 hours at 10°C.

公开(公告)号：EP3778625A1

公开(公告)日：2021-02-17

申请号：EP19782446.9

申请日：2019-03-28

申请人： Godo Shusei Co., Ltd.

发明人： ODAKA, Rei ,  BABA, Yasuhiro ,  OGASAWARA, Junki ,  YOSHIKAWA, Jun

IPC分类号： C07K14/195 ,  C07H3/06 ,  C12N1/13 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/56 ,  C12P19/04

摘要： [Problem]
To provide: a novel enzyme which is derived from a microorganism other than Bacillus circulans and is highly selective for galactotrisaccharide production and with which the galactooligosaccharide can be highly efficiently produced; and a novel method capable of producing the galactooligosaccharide. [Solution] A method for producing a galatooligosaccharide, the method including bringing an enzyme having an amino acid sequence selected from the group consisting of (a) to (f) and cells of a bacterium belonging to the genus Paenibacillus and/or a galactooligosaccharide-producing enzyme for the bacterium, into contact with lactose,
(a) An amino acid sequence of sequence number 1.
(b) An amino acid sequence of sequence number 2.
(c) An amino acid sequence of an enzyme having galactooligosaccharide-producing activity, the amino acid sequence being the amino acid sequence of sequence number 1 in which one to ten amino acids have been replaced, removed, or inserted,
(d) An amino acid sequence of an enzyme having galactooligosaccharide-producing activity, the amino acid sequence being the amino acid sequence of sequence number 2 in which one to ten amino acids have been replaced, removed, or inserted,
(e) An amino acid sequence of an enzyme having galactooligosaccharide-producing activity, the amino acid sequence having a homology of 80% or higher but less than 100% to the amino acid sequence of sequence number 1.
(f) An amino acid sequence of an enzyme having galactooligosaccharide-producing activity, the amino acid sequence having a homology of 80% or higher but less than 100% to the amino acid sequence of sequence number 2.