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公开(公告)号：EP3228705A1
公开(公告)日：2017-10-11
申请号：EP15864800.6
申请日：2015-12-04
发明人： HORIGUCHI, Hirofumi , IYOTANI, Ai , SHIOTA, Kazuma
CPC分类号： C12N9/2411 , C12N5/10 , C12N15/09 , C12N15/70 , C12N15/746 , C12N15/81 , C12P19/12 , C12P19/16 , C12P19/22 , C12Y302/01068 , C13K7/00
摘要： An isoamylase having improved heat resistance and an industrial method for producing maltose from starch.
The isoamylase is an isoamylase consisting of the amino acid sequence represented by SEQ ID NO: 1 or an isoamylase resulting from deletion, substitution, or insertion of one to several amino acid residues in the amino acid sequence represented by SEQ ID NO: 1, wherein at least valine at amino acid number 515 and methionine at amino acid number 570 are mutated to other amino acids.摘要翻译： 具有改进的耐热性的异淀粉酶和由淀粉生产麦芽糖的工业方法。 异淀粉酶是由序列号1表示的氨基酸序列组成的异淀粉酶，或由序列号1表示的氨基酸序列中的1〜数个氨基酸残基的缺失，置换或插入而得到的异淀粉酶， 至少氨基酸号515的缬氨酸和氨基酸号570的甲硫氨酸突变为其他氨基酸。
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公开(公告)号：EP2687598A1
公开(公告)日：2014-01-22
申请号：EP12758277.3
申请日：2012-03-13
CPC分类号： C12Q1/34 , A23C9/1206 , C12N9/16 , C12N9/2402 , C12N9/2468 , C12N9/2471 , C12Q1/44 , C12Y301/06001 , C12Y302/01023 , C12Y302/01108 , G01N2333/916
摘要： Provided is a method for assaying arylsulfatase activity in aqueous system, which comprises a step for reacting, in an aqueous reaction system with a high ionic strength, arylsulfatase with a substrate thereof, said substrate releasing a fluorophore or a chromophore when subjected to the action of arylsulfatase. Also provided are: a lactase preparation having a lactase activity of 4,000 NLU/g or greater, said lactase activity being assayed by the FCC IV method, and an arylsulfatase activity not greater than 0.1%, said arylsulfatase activity being assayed by the fluorescence method according to the present invention for assaying arylsulfatase activity in aqueous system on the basis of the lactase activity; a method for producing said preparation; and a dairy product with the use of said preparation.
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公开(公告)号：EP2998395B1
公开(公告)日：2019-04-10
申请号：EP14798365.4
申请日：2014-05-12
发明人： YOSHIKAWA, Jun , SHIOTA, Kazuma
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公开(公告)号：EP2687598B1
公开(公告)日：2017-02-15
申请号：EP12758277.3
申请日：2012-03-13
IPC分类号： C12N15/09
CPC分类号： C12Q1/34 , A23C9/1206 , C12N9/16 , C12N9/2402 , C12N9/2468 , C12N9/2471 , C12Q1/44 , C12Y301/06001 , C12Y302/01023 , C12Y302/01108 , G01N2333/916
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公开(公告)号：EP2998395A1
公开(公告)日：2016-03-23
申请号：EP14798365.4
申请日：2014-05-12
发明人： YOSHIKAWA, Jun , SHIOTA, Kazuma
CPC分类号： A23C9/1206 , A23V2002/00 , C12N9/2402 , C12N9/2468 , C12Y302/01108 , A23V2300/30
摘要： A method for producing a lactase-containing composition which is purified by selectively removing protease contaminating the lactase using simple and easy means; a lactase-containing composition; and a dairy product containing the lactase-containing composition.
A method for producing a lactase-containing composition having a reduced protease content includes: dissolving a composition containing lactase and protease in an aqueous salt solution having an electric conductivity of from 2 to 45 mS/cm; bringing the resultant solution into contact with an ion exchange resin; and collecting a fraction which is not adsorbed onto the ion exchange resin.摘要翻译： 一种生产含乳糖酶组合物的方法，所述组合物通过使用简单和容易的手段选择性地去除污染乳糖酶的蛋白酶而被纯化; 含乳糖酶的组合物; 和含有含乳糖酶组合物的乳制品。 生产蛋白酶含量降低的含有乳糖酶的组合物的方法包括：将含有乳糖酶和蛋白酶的组合物溶解在电导率为2-45mS / cm的盐水溶液中; 使得到的溶液与离子交换树脂接触; 并收集未被吸附到离子交换树脂上的部分。
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公开(公告)号：EP2503333A1
公开(公告)日：2012-09-26
申请号：EP10831568.0
申请日：2010-11-17
发明人： SHIOTA, Kazuma , FUJITA, Tomohiro
IPC分类号： G01N33/483 , C12N9/54 , G01N27/447
CPC分类号： C12N9/52 , C07K1/26 , G01N27/447
摘要： Provided is a means for accurately analyzing a protease by electrophoresis.
Disclosed is an electrophoretic analysis method for analyzing a protease-containing sample, is characterized by exposing a sample containing a protease to be analyzed, to pH conditions under which the protease is rapidly deactivated, and then subjecting the sample to electrophoresis.摘要翻译： 提供了通过电泳准确分析蛋白酶的方法。 公开了一种用于分析含蛋白酶的样品的电泳分析方法，其特征在于将含有待分析蛋白酶的样品暴露于蛋白酶快速失活的pH条件下，然后对样品进行电泳。
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7.发明公开
公开(公告)号：EP4379049A1
公开(公告)日：2024-06-05
申请号：EP22849552.9
申请日：2022-07-27
发明人： IIZAKA MORIE, Kyoko , ARAYA, Naho , SHIOTA, Kazuma
CPC分类号： C12N9/0004 , A23C7/04 , C12N9/50 , A23C9/152 , A23C9/13
摘要： To provide an enzyme-containing composition, less likely to generate curds by the action of peroxidase even when the peroxidase is reacted with the milk for a long time, a method for producing milk, and a method for producing fermented milk. An enzyme-containing composition, in which a ratio of the protease activity to the peroxidase activity is 10 or less.
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公开(公告)号：EP2503333B1
公开(公告)日：2016-08-03
申请号：EP10831568.0
申请日：2010-11-17
发明人： SHIOTA, Kazuma , FUJITA, Tomohiro
IPC分类号： G01N33/483 , C12N9/54 , G01N27/447
CPC分类号： C12N9/52 , C07K1/26 , G01N27/447
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