公开(公告)号：EP3008168A4

公开(公告)日：2017-05-31

申请号：EP14819763

申请日：2014-06-11

申请人： HARVARD COLLEGE ,  JANSSEN BIOTECH INC

发明人： PETERSON QUINN P ,  PAGLIUCA FELICIA J ,  MELTON DOUGLAS A ,  MILLMAN JEFFREY R ,  SEGEL MICHAEL SARIS ,  GÜRTLER MADS ,  REZANIA ALIREZA ,  FRYER BENJAMIN

IPC分类号： C12N5/02 ,  C12N5/00 ,  C12N5/071 ,  C12N5/0735 ,  C12N5/074

CPC分类号： C12N5/0678 ,  C12N5/0606 ,  C12N5/0676 ,  C12N5/0696 ,  C12N2501/11 ,  C12N2501/117 ,  C12N2501/15 ,  C12N2501/16 ,  C12N2501/375 ,  C12N2501/385 ,  C12N2501/395 ,  C12N2501/40 ,  C12N2501/41 ,  C12N2501/727 ,  C12N2501/998 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/22 ,  C12N2506/45

摘要： Disclosed herein is a method for differentiating PDX1-positive pancreatic progenitor cells into NKX6.1 positive pancreatic progenitor cells with a culture medium comprising a) KGF and b) SANT1, wherein the culture medium is substantially free of (1) a Protein Kinase C (PKC) Activator or (2) a BMP signaling pathway inhibitor.

摘要翻译： 本文公开了用于诱导β细胞成熟的方法，组合物，试剂盒和试剂，以及用于各种应用（例如细胞疗法）的分离的SC-β细胞群。

公开(公告)号：EP2611909A4

公开(公告)日：2014-03-05

申请号：EP11822349

申请日：2011-08-17

申请人： JANSSEN BIOTECH INC

发明人： FRYER BENJAMIN

IPC分类号： C12N5/0735 ,  C12N5/02 ,  C12N5/071 ,  C12N5/074

CPC分类号： C12N5/0678 ,  C12N2500/34 ,  C12N2500/90 ,  C12N2501/115 ,  C12N2501/19 ,  C12N2501/727 ,  C12N2506/02 ,  C12N2533/54

摘要： The present invention provides methods to promote the differentiation of pluripotent stem cells into insulin producing cells. In particular, the present invention provides a method to produce a population of cells, wherein greater than 80% of the cells in the population express markers characteristic of the definitive endoderm lineage.

公开(公告)号：EP2494035A4

公开(公告)日：2013-09-25

申请号：EP10830437

申请日：2010-10-28

申请人： JANSSEN BIOTECH INC

发明人： FUNG RAMIE ,  FRYER BENJAMIN

IPC分类号： C12N5/074 ,  C12N5/02

CPC分类号： C12N5/0696 ,  C12N2500/90 ,  C12N2501/115 ,  C12N2501/117 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/605 ,  C12N2501/608 ,  C12N2501/70 ,  C12N2502/13 ,  C12N2506/02 ,  C12N2510/00 ,  C12N2533/90 ,  C12N2799/027

摘要： The present invention provides methods to produce pluripotent stem cells from adult cells. In particular, the present invention provides methods to produce pluripotent stem cells from somatic cells without the use of a feeder-cell layer or an agent that increases efficiency of retroviral transfection.

摘要翻译： 本发明提供从成体细胞产生多能干细胞的方法。 特别地，本发明提供了从体细胞产生多能干细胞而不使用饲养细胞层或提高逆转录病毒转染效率的试剂的方法。

公开(公告)号：EP3063268A4

公开(公告)日：2017-11-29

申请号：EP14857034

申请日：2014-05-21

申请人： JANSSEN BIOTECH INC

发明人： FRYER BENJAMIN ,  LANIAUSKAS DAINA ,  BLACKMOORE MARCIA ,  WANG HAIYUN ,  LILOVA KOSTADINKA ,  NELSON SHELLEY ,  ROSOCHA ELIZABETH

IPC分类号： C12N5/071 ,  A61K31/41 ,  C07D403/00 ,  C12N5/02 ,  C12N5/0735 ,  C12N5/077 ,  C12N5/079

CPC分类号： C12N5/0606 ,  C12N5/0618 ,  C12N5/0657 ,  C12N5/0676 ,  C12N2500/02 ,  C12N2501/117 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/19 ,  C12N2501/385 ,  C12N2501/41 ,  C12N2501/415 ,  C12N2501/42 ,  C12N2501/727 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/45 ,  C12N2509/00 ,  C12N2513/00 ,  C12N2531/00

摘要： The present invention provides methods of preparing aggregated pluripotent stem cell clusters for differentiation. Specifically, the invention discloses methods of differentiating pluripotent cells into beta cell, cardiac cell and neuronal cell lineages using suspension clustering. The methods involve preparing the aggregated cell clusters followed by differentiation of these clusters.

公开(公告)号：EP2938722A4

公开(公告)日：2017-03-22

申请号：EP13868527

申请日：2013-12-30

申请人： JANSSEN BIOTECH INC

发明人： FRYER BENJAMIN ,  LANIAUSKAS DAINA ,  BLACKMOORE MARCIA ,  WANG HAIYUN ,  LILOVA KOSTADINKA ,  ROSOCHA ELIZABETH ,  NELSON SHELLEY

IPC分类号： C12N5/071 ,  C12N5/02 ,  C12N5/0735

CPC分类号： C12N5/0677 ,  C12N5/0606 ,  C12N2500/02 ,  C12N2500/25 ,  C12N2500/34 ,  C12N2501/117 ,  C12N2501/15 ,  C12N2501/16 ,  C12N2501/19 ,  C12N2501/385 ,  C12N2501/415 ,  C12N2501/42 ,  C12N2501/727 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/45 ,  C12N2509/00 ,  C12N2513/00 ,  C12N2531/00

摘要： The present invention provides methods of preparing aggregated pluripotent stem cell clusters for differentiation.

公开(公告)号：EP3234109A4

公开(公告)日：2018-06-27

申请号：EP15870718

申请日：2015-12-09

申请人： JANSSEN BIOTECH INC

发明人： FRYER BENJAMIN ,  LANIAUSKAS DAINA

IPC分类号： C12N5/071 ,  C12N5/02

CPC分类号： C12N5/0676 ,  C12N2500/02 ,  C12N2500/25 ,  C12N2500/38 ,  C12N2500/60 ,  C12N2501/117 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/19 ,  C12N2501/385 ,  C12N2501/395 ,  C12N2501/41 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2501/91 ,  C12N2501/999 ,  C12N2506/02

摘要： The present invention provides methods of differentiating pluripotent cells into beta cell using suspension clustering. The methods of the invention use control of one or more of pH, cell concentration, and retinoid concentration to generate a nearly homogenous population of PDX1/NKX6.1 co-expressing cells by suppressing precocious NGN3 expression and promoting NKX6.1 expression. Also, the nearly homogenous population of PDX1/NKX6.1 co-expressing cells may be further differentiated in vitro to form a population of pancreatic endocrine cells that co-express PDX1, NKX6.1, insulin and MAFA.

公开(公告)号：EP3063269A4

公开(公告)日：2017-07-12

申请号：EP14859059

申请日：2014-06-17

申请人： JANSSEN BIOTECH INC

发明人： FRYER BENJAMIN ,  LANIAUSKAS DAINA ,  BLACKMOORE MARCIA ,  WANG HAIYUN ,  LILOVA KOSTADINKA ,  NELSON SHELLEY ,  ROSOCHA ELIZABETH

IPC分类号： C12N5/071 ,  A61K31/41 ,  C07D403/00 ,  C12N5/02 ,  C12N5/0735 ,  C12N5/077 ,  C12N5/079

CPC分类号： C12N5/0606 ,  C12N5/0618 ,  C12N5/0657 ,  C12N5/0676 ,  C12N2500/02 ,  C12N2501/117 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/19 ,  C12N2501/385 ,  C12N2501/41 ,  C12N2501/415 ,  C12N2501/42 ,  C12N2501/727 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/45 ,  C12N2509/00 ,  C12N2513/00 ,  C12N2531/00

摘要： The present invention provides methods of preparing aggregated pluripotent stem cell clusters for differentiation. Specifically, the invention discloses methods of differentiating pluripotent cells into beta cell, cardiac cell and neuronal cell lineages using suspension clustering. The methods involve preparing the aggregated cell clusters followed by differentiation of these clusters.

摘要翻译： 本发明提供了制备用于分化的聚集的多能干细胞簇的方法。 具体而言，本发明公开了使用悬浮聚类将多能细胞分化成β细胞，心脏细胞和神经元细胞谱系的方法。 这些方法涉及制备聚集的细胞簇，然后分化这些簇。