公开(公告)号：EP1041151A4

公开(公告)日：2002-09-18

申请号：EP98961523

申请日：1998-12-24

申请人： KIKKOMAN CORP

发明人： HATTORI NORIAKI ,  MURAKAMI SEIJI

IPC分类号： C12N15/09 ,  C12N1/21 ,  C12N9/02 ,  C12N15/53 ,  C12Q1/66 ,  C12R1/19 ,  C12N15/63 ,  C12Q1/26

CPC分类号： C12N9/0069 ,  C12Q1/66

摘要： A luciferase tolerant to surfactants; and a method for assaying intracellular ATP which comprises the first step of extracting ATP from a cell-containing sample in the presence of a surfactant, the second step of adding a luciferase-containing luminescent reagent to the ATP extract thus inducing luminescence and the third step of detecting the luminescence dose, characterized by using the luciferase tolerant to surfactants.

公开(公告)号：EP1024354A4

公开(公告)日：2004-08-04

申请号：EP98947821

申请日：1998-10-12

申请人： KIKKOMAN CORP

发明人： IGARASHI TOSHINORI ,  HATTORI NORIAKI ,  MURAKAMI SEIJI ,  SAKAKIBARA TATSUYA ,  SAITO MORISAKU

IPC分类号： C12Q1/02 ,  A61B10/00 ,  B01L3/00 ,  B01L3/14 ,  G01N1/02 ,  G01N1/04 ,  G01N1/12 ,  G01N33/48 ,  G01N1/08

CPC分类号： B01L3/5029 ,  A61B10/0045 ,  B01L3/5082 ,  B01L2300/047 ,  B01L2300/0672 ,  B01L2400/0683 ,  G01N1/02 ,  G01N2001/028 ,  Y10T436/25

摘要： A wiping inspection instrument comprising a sample collecting member and a reaction reagent container. The sample collecting member comprises a tubular main body having both upper and lower ends thereof opened, a sample wiping member removably fitted to the upper open portion of the main body and an extraction liquid container disposed below the main body. The sample wiping member includes a retaining member inserted into the upper part of the main body and a cotton buds stick held by the retaining member. Further, the sample collecting member includes a splash-proofing member disposed below the extraction liquid container, for preventing the splash of the extraction liquid. The reaction reagent container has a test tube shape and is so inserted into the open portion at the lower end of the main body as to be capable of moving up and down.

摘要翻译： 一种擦拭检查装置，包括样本收集部件和反应试剂容器。 样品收集部件包括其上端和下端均敞开的管状主体，可拆卸地装配到主体上部开口部分的样品擦拭部件和设置在主体下方的提取液体容器。 样本擦拭部件包括插入主体上部的保持部件和由保持部件保持的棉签棒。 此外，样本收集构件包括设置在提取液容器下方的防溅构件，用于防止提取液的飞溅。 反应试剂容器具有试管形状，并插入主体下端的开口部分，以便能够上下移动。

公开(公告)号：EP1143012A4

公开(公告)日：2004-10-06

申请号：EP99961365

申请日：1999-12-24

申请人： KIKKOMAN CORP

发明人： SAKAKIBARA TATSUYA ,  IGARASHI TOSHINORI ,  MURAKAMI SEIJI ,  HAKETA YASUSHI

IPC分类号： C12M1/26 ,  C12M1/34 ,  C12Q1/00 ,  C12Q1/48 ,  C12Q1/22 ,  C12Q1/66

CPC分类号： C12Q1/008 ,  C12Q1/485 ,  G01N21/763

摘要： A method for measuring stains of a specimen by measuring adenosine phosphates. Luminescence of the adenosine phosphates is induced by a luminescent reagent (33) containing an ATP regenerating enzyme. Then the luminescence dose is detected with a silicone photodiode (5) to thereby measure the stains.

公开(公告)号：EP1167968A4

公开(公告)日：2006-05-03

申请号：EP00912914

申请日：2000-03-29

申请人： KIKKOMAN CORP

发明人： IGARASHI TOSHINORI ,  MURAKAMI SEIJI ,  SAITO MORISAKU

IPC分类号： G01N33/48 ,  B01L3/00 ,  B01L3/14 ,  C12M1/30 ,  G01N33/53

CPC分类号： B01L3/5029 ,  B01L3/5082 ,  B01L2400/0683 ,  G01N33/5304

摘要： A sample examining instrument comprising a test-tube-form measuring container storing therein a reaction reagent such as an emission reagent, and a reagent container inserted for burying into the upper opening of the measuring container, wherein sealants are pasted to the upper and lower portions of the reagent container and a reagent such as an extract liquid is sealed in the reagent container, whereby providing the sample examining instrument downsized due to the reagent container fitted into the measuring container.

公开(公告)号：EP1048739A4

公开(公告)日：2004-06-30

申请号：EP98956015

申请日：1998-12-01

申请人： KIKKOMAN CORP

发明人： HATTORI NORIAKI ,  YAJITATE KEIKO ,  NAKAJIMA MOTOO ,  MURAKAMI SEIJI

IPC分类号： G01N33/48 ,  C12Q1/06 ,  C12Q1/66 ,  C12Q1/68 ,  G01N33/66

CPC分类号： G01N33/66 ,  C12Q1/66 ,  C12Q2304/60 ,  G01N2400/16 ,  G01N2400/18

摘要： A method for analyzing intracellular components characterized by involving the following steps; and kits containing (a) an extractant, (b) a branched dextrin or its derivative, and (c) a reagent for analyzing intracellular components: (1) the step of adding the extractant to a sample containing cells to thereby extract intracellular components; (2) the step of adding the branched dextrin or its derivative to the sample containing the extractant; and (3) the step of analyzing the thus extracted intracellular components. The use of the branched dextrin or its derivative makes it possible to prevent the extractant from inhibiting the analysis steps.

摘要翻译： 一种分析细胞内成分的方法，其特征在于包括以下步骤： 和（a）提取剂，（b）支链糊精或其衍生物的试剂盒和（c）用于分析细胞内成分的试剂：（1）将提取剂加入到含有细胞的样品中从而提取细胞内成分的步骤; （2）将分支糊精或其衍生物加入含有萃取剂的样品的步骤; 和（3）分析由此提取的细胞内组分的步骤。 使用分支糊精或其衍生物可以防止萃取剂抑制分析步骤。

公开(公告)号：EP1048739A1

公开(公告)日：2000-11-02

申请号：EP98956015

申请日：1998-12-01

申请人： KIKKOMAN CORP

发明人： HATTORI NORIAKI ,  YAJITATE KEIKO ,  NAKAJIMA MOTOO ,  MURAKAMI SEIJI

IPC分类号： G01N33/48 ,  C12Q1/06 ,  C12Q1/66 ,  C12Q1/68 ,  G01N33/66

CPC分类号： G01N33/66 ,  C12Q1/66 ,  C12Q2304/60 ,  G01N2400/16 ,  G01N2400/18

摘要： A method for analyzing intracellular components characterized by involving the following steps; and kits containing (a) an extractant, (b) a branched dextrin or its derivative, and (c) a reagent for analyzing intracellular components: (1) the step of adding the extractant to a sample containing cells to thereby extract intracellular components; (2) the step of adding the branched dextrin or its derivative to the sample containing the extractant; and (3) the step of analyzing the thus extracted intracellular components. The use of the branched dextrin or its derivative makes it possible to prevent the extractant from inhibiting the analysis steps.