公开(公告)号：EP4403186A1

公开(公告)日：2024-07-24

申请号：EP22870048.0

申请日：2022-09-16

申请人： Keio University

发明人： YASUDA Hiroyuki ,  SATO Toshiro ,  EBISUDANI Toshiki ,  FUKUSHIMA Takahiro ,  FUKUNAGA Koichi ,  HAMAMOTO Junko

IPC分类号： A61K45/00 ,  A61P11/00 ,  A61P35/00 ,  C12N5/09 ,  C12N1/00 ,  G01N33/574 ,  A61K31/4985

CPC分类号： A61K31/4985 ,  A61K45/00 ,  A61P11/00 ,  A61P35/00 ,  C12N1/00 ,  C12N5/06 ,  G01N33/574

摘要： A therapeutic agent for small cell lung cancer containing an insulin-like growth factor 1 receptor (IGF1R) inhibitor as an active ingredient, and a method whether or not an administration of the therapeutic agent for small cell lung cancer patients is effective, including: a step of detecting the expression of YAP1 or its downstream factors in the cancer cells from the small cell lung cancer patient; and a step of detecting the expression of YAP1 or its downstream factors in the cancer cells from the small cell lung cancer patient, wherein when the expression level of YAP1 or the expression level of the downstream factor of YAP1 is significantly higher than that of a control, the administration of the therapeutic agent is effective in treating the patient with small cell lung cancer.

公开(公告)号：EP4257672A1

公开(公告)日：2023-10-11

申请号：EP21900710.1

申请日：2021-12-03

申请人： Keio University ,  JSR Corporation

发明人： SATO Toshiro ,  SUGIMOTO Shinya ,  ARAI Kazuya

IPC分类号： C12N5/071 ,  C12Q1/06

摘要： A method for producing a two-dimensional small intestinal organoid having a villus structure, the method including a step 1 of culturing a cell derived from a small intestinal epithelium in an extracellular matrix to obtain a three-dimensional small intestinal organoid, a step 2 of dispersing the three-dimensional small intestinal organoid and monolayer culturing on the extracellular matrix to obtain a two-dimensional small intestinal organoid, and a step 3 of further culturing the two-dimensional small intestinal organoid while letting a culture medium of the two-dimensional small intestinal organoid to flow so that the two-dimensional small intestinal organoid forms a villus structure.

公开(公告)号：EP3903791A1

公开(公告)日：2021-11-03

申请号：EP19905829.8

申请日：2019-12-27

申请人： Keio University ,  The Kitasato Institute

发明人： KATAYAMA Kazuhiko ,  FUJIMOTO Akira ,  HAGA Kei ,  TODAKA Reiko ,  SATO Toshiro

IPC分类号： A61K31/7004 ,  A61K31/7024 ,  A61P31/14

摘要： An object of the present invention is to provide an anti-human norovirus agent which can inhibit infection with and proliferation of human norovirus. The present inventors have found that the object can be attained through provision of an anti-human norovirus agent comprising, as an active ingredient, a fucose analog having an inhibitory action on glycosylation by fucosyltransferase (FUT). Examples of the active ingredient include a fucose analog represented by formula (III) or (IV) or a salt thereof.

[In the formulas, each of formula (III) and formula (IV) represents an α-anomer or a β-anomer; each of R 1 , R 3 , and R 4 is -OH or -OAc; R 2 is a halogen atom, -OH, or -OAc; and R 5 is -CH 3 , -C=CH, -C≡CCH 3 , or -CH 2 C≡CH.]

公开(公告)号：EP3460042A1

公开(公告)日：2019-03-27

申请号：EP17799236.9

申请日：2017-05-10

申请人： Keio University

发明人： SATO Toshiro ,  MATANO Mami

IPC分类号： C12N1/00 ,  C12N5/071 ,  C12N5/074 ,  C12N5/09 ,  C07K14/475 ,  C07K14/51

摘要： A cell culture medium for culturing organoid containing at least two types of components selected from the group consisting of insulin-like growth factor 1 (IGF1), fibroblast growth factor 2 (FGF2) and epiregulin (EREG), and at least one type of component among the following components i) to iii): i) Wnt agonist, ii) bone morphogenetic protein (BMP) inhibitor, and iii) transforming growth factor-β (TGF-β) inhibitor.

公开(公告)号：EP3974514A1

公开(公告)日：2022-03-30

申请号：EP20809811.1

申请日：2020-03-23

申请人： Keio University

发明人： SATO Toshiro ,  SASAKI Nobuo

IPC分类号： C12M1/00 ,  C12N5/071 ,  C12N1/20

摘要： A culture container for culturing epithelial cells, includes an upper container, a lid member configured to airtightly fit with an opening portion of the upper container, and a lower container configured to accommodate the upper container and a cell culture medium, in which at least a part of an area of the upper container in contact with the cell culture medium is formed of a membrane that is permeable to at least a part of components of the cell culture medium and impermeable to a cell, and a material of the lid member has an oxygen permeability coefficient of 1.0 × 10 -6 cm 3 cm/(cm 2 ·sec·atm) or less.

公开(公告)号：EP3418376A1

公开(公告)日：2018-12-26

申请号：EP17753324.7

申请日：2017-02-17

申请人： Keio University ,  Osaka University

发明人： SATO Toshiro ,  MATANO Mami ,  SUGIMOTO Shinya ,  TAKAGI Junichi

IPC分类号： C12N5/00 ,  C12N5/0775 ,  C12N5/09 ,  C12N5/10

CPC分类号： C12N5/00 ,  C12N5/10

摘要： The present invention provides a cell culture medium with which serum-free long-term culture of an epithelial stem cell, an epithelial cancer cell, or a tissue containing at least one thereof can be achieved. The cell culture medium of the present invention includes: a Wnt agonist composed of a complex of Wnt protein and afamin, which is a substance capable of stabilizing the Wnt protein, and R-spondin; and at least one selected from the group consisting of a mitogenic growth factor, a bone morphogenetic protein (BMP) inhibitor, a transforming growth factor-β (TGF-β) inhibitor, and a p38 inhibitor.