公开(公告)号：EP1594977A4

公开(公告)日：2009-01-28

申请号：EP04712281

申请日：2004-02-18

申请人： MIRUS BIO CORP

发明人： ROZEMA DAVID B ,  WAKEFIELD DARREN

IPC分类号： A01N43/04 ,  A61K9/127 ,  A61K47/48 ,  A61K48/00 ,  C12N15/11 ,  C12Q20060101 ,  C12Q1/00

CPC分类号： C12N15/111 ,  A61K47/58 ,  A61K48/00 ,  A61K48/0041 ,  C12N2310/351 ,  C12N2320/32

摘要： Described is a process for delivering a biologically active compound to a cell by reversibly linking the compound to a membrane active polymer. In particular, polymer-polynucleotide conjugates are described. Methods for reversibly modifying the polymers to decrease cellular toxicity and improve efficacy are provided.

公开(公告)号：EP1660099A4

公开(公告)日：2007-01-03

申请号：EP03778146

申请日：2003-11-05

申请人： MIRUS BIO CORP

发明人： WOLFF JON A ,  HAGSTROM JAMES E ,  BUDKER VLADIMIR G ,  ROZEMA DAVID B ,  MONAHAN SEAN D ,  SLATTUM PAUL M

IPC分类号： A61K31/70 ,  A61K31/7088 ,  A61K38/18 ,  A61K47/48 ,  A61K48/00 ,  C07H21/00

CPC分类号： A61K48/005 ,  A61K31/7088 ,  A61K38/1808 ,  A61K38/1825 ,  A61K47/59 ,  A61K47/62 ,  A61K47/645 ,  A61K48/0008 ,  A61K48/0016 ,  A61K48/0025 ,  A61K48/0041 ,  A61K48/0075 ,  A61K48/0083 ,  C07H21/00 ,  A61K2300/00

摘要： Disclosed is a process for delivering genetic material into a mammalian cell to alter endogenous properties of the cell. The process comprises designing a polynucleotide for transfection. Then the polynucleotide is inserted into a mammalian vessel such as an artery. Prior to insertion, subsequent to insertion, or concurrent with insertion the permeability of the vessel is increased thereby the genetic material is delivered to the parenchymal cell altering endogenous properties of the cell.

摘要翻译： 公开了将遗传物质递送到哺乳动物细胞中以改变细胞的内源性质的方法。 该过程包括设计用于转染的多核苷酸。 然后将多核苷酸插入哺乳动物血管例如动脉中。 在插入之前，插入之后或与插入同时，血管的渗透性增加，由此将遗传物质递送至实质细胞，从而改变细胞的内源性质。

公开(公告)号：EP1507874A4

公开(公告)日：2006-06-28

申请号：EP03734207

申请日：2003-05-28

申请人： MIRUS BIO CORP

发明人： LEWIS DAVID L ,  ROZEMA DAVID B ,  WAKEFIELD DARREN ,  HERWEIJER HANS ,  WOLFF JON A ,  HAGSTROM JAMES E

IPC分类号： A01N43/04 ,  A61K31/07 ,  A61K47/48 ,  C07H21/04 ,  C12N15/113 ,  C12N15/87 ,  C12Q1/68

CPC分类号： C12N15/87 ,  A61K47/59 ,  A61K47/645 ,  A61K47/6931 ,  C12N15/113 ,  C12N2310/3145 ,  C12N2310/3181 ,  C12N2310/3233

摘要： Compositions are provided for delivery of polynucleotides to cells for the purpose of inhibiting gene expression. Antisense polynucleotide-containing complexes are described. The salt and serum stability and small size of the complexes permits delivery to cells in vitro and in vivo.

公开(公告)号：EP1651239A4

公开(公告)日：2006-12-20

申请号：EP03814414

申请日：2003-07-22

申请人： MIRUS BIO CORP

发明人： WOLFF JON A ,  HAGSTROM JAMES E ,  BUDKER VLADIMIR G ,  ROZEMA DAVID B ,  MONAHAN SEAN D ,  SLATTUM PAUL M

IPC分类号： A61K48/00 ,  A61K31/70 ,  C12N15/113

CPC分类号： C12N15/113 ,  A61K31/70 ,  A61K48/0025 ,  A61K48/0041 ,  A61K48/0075 ,  A61K48/0083 ,  C12N2320/32 ,  A61K2300/00

摘要： Disclosed is a process for transfecting genetic material into a mammalian cell to alter endogenous properties of the cell. The process comprises designing a polynucleotide for transfection. Then the polynucleotide is inserted into a mammalian vessel such as a tail vein or artery. Prior to insertion, subsequent to insertion, or concurrent with insertion the permeability of the vessel is increased thereby the genetic material is delivered to the parenchymal cell altering endogenous properties of the cell.

公开(公告)号：EP1589999A4

公开(公告)日：2010-05-19

申请号：EP04709060

申请日：2004-02-06

申请人： MIRUS BIO CORP

发明人： ROZEMA DAVID B ,  WAKEFIELD DARREN

IPC分类号： A61K9/00 ,  A61K47/32 ,  A61K47/48 ,  A61K48/00 ,  C12N15/87 ,  A61K47/30 ,  A61K47/34 ,  C12N15/11 ,  C12N15/63

CPC分类号： C12N15/87 ,  A61K9/0019 ,  A61K47/32 ,  A61K47/58 ,  A61K48/0008 ,  A61K48/0041

摘要： A class of polymers for delivery of polynucleotides to cells in described. More specifically, amphiphilic polyvinylethers and compositions containing amphiphilic polyvinylethers are described.

摘要翻译： 一类用于将多核苷酸递送至所述细胞的聚合物。 更具体地，描述了两亲聚乙烯醚和含有两亲性聚乙烯醚的组合物。

公开(公告)号：EP1646282A4

公开(公告)日：2006-10-11

申请号：EP04779025

申请日：2004-07-17

申请人： MIRUS BIO CORP

发明人： HERWEIJER HANS ,  BATES MARY KAY ,  WOLFF JON A ,  BUDKER VLADIMIR G ,  HAGSTROM JAMES E ,  MONAHAN SEAN D ,  SLATTUM PAUL M ,  ROZEMA DAVID B

IPC分类号： A01N43/04 ,  A01N59/16 ,  A01N59/26 ,  A61K31/70 ,  A61K39/00 ,  A61K39/29 ,  A61K47/48 ,  A61K48/00 ,  C07H21/00 ,  C07K14/135 ,  C07K14/47 ,  C07K14/475 ,  C07K14/73 ,  C07K16/00 ,  C07K16/18 ,  C12N5/00 ,  C12N5/02 ,  C12N5/16 ,  C12N9/02 ,  C12N15/113 ,  C12N15/63 ,  C12N15/85

CPC分类号： C07K16/18 ,  A01N59/16 ,  A01N59/26 ,  A61K31/70 ,  A61K39/00 ,  A61K39/0011 ,  A61K47/58 ,  A61K47/59 ,  A61K47/62 ,  A61K47/645 ,  A61K48/00 ,  A61K48/0008 ,  A61K48/0075 ,  A61K2039/53 ,  C07H21/00 ,  C07K14/005 ,  C07K14/4708 ,  C07K14/475 ,  C07K14/70514 ,  C07K16/00 ,  C12N9/0069 ,  C12N15/113 ,  C12N15/87 ,  C12N2320/32 ,  C12N2760/18522 ,  A01N2300/00 ,  A61K2300/00

公开(公告)号：EP1506218A4

公开(公告)日：2010-08-11

申请号：EP03755460

申请日：2003-05-23

申请人： MIRUS BIO CORP

发明人： ROZEMA DAVID B ,  WOLFF JON A ,  WAKEFIELD DARREN ,  EKENA KIRK ,  HAGSTROM JAMES E

IPC分类号： C07D307/60 ,  A61K38/00 ,  A61K45/00 ,  A61K47/48 ,  A61K48/00 ,  C07K7/06 ,  A61P35/00 ,  C07K1/107

CPC分类号： C07K1/1072 ,  A61K47/60

公开(公告)号：EP1841864A4

公开(公告)日：2008-11-12

申请号：EP06718148

申请日：2006-01-12

申请人： MIRUS BIO CORP

发明人： WAKEFIELD DARREN H ,  ROZEMA DAVID B ,  WOLFF JON A ,  TRUBETSKOY VLADIMIR ,  HAGSTROM JAMES E ,  BUDKER VLADIMIR G ,  KLEIN JASON ,  WONG SO

IPC分类号： C12N15/00 ,  C12N15/09 ,  C12N15/70 ,  C12N15/74

CPC分类号： C12N15/87 ,  A61K48/0091

摘要： Polyampholyte are able to condense nucleic acid to form small complexes which can be utilized in the delivery of nucleic acid to mammalian cells. The polyampholytes can be formed prior to interaction with nucleic acid or they can be formed in the presence of nucleic acid. Stabilized polycation/nucleic acid complexes can be modified to reduce the positive charge of the polycation and add targeting ligands without destabilizing the complex. The resultant particles retain their small size and are more effective in delivery of nucleic acid to cells in vivo.

公开(公告)号：EP1506020A4

公开(公告)日：2007-08-29

申请号：EP03751760

申请日：2003-05-19

申请人： MIRUS BIO CORP

发明人： LEWIS DAVID L ,  ROZEMA DAVID B ,  WOLFF JON A ,  HAGSTROM JAMES E ,  HERWEIJER HANS

IPC分类号： A61K38/00 ,  C12N15/11 ,  A61K48/00 ,  C07H21/00 ,  C12Q1/68

CPC分类号： C12N15/111 ,  A61K38/00 ,  C12N2310/11 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/3233 ,  C12N2310/53 ,  C12N2320/31

公开(公告)号：EP1667728A4

公开(公告)日：2007-02-28

申请号：EP03810873

申请日：2003-08-18

申请人： MIRUS BIO CORP

发明人： WOLFF JON A ,  HAGSTROM JAMES E ,  BUDKER VLADIMIR G ,  ROZEMA DAVID B ,  MONAHAN SEAN D ,  SLATTUM PAUL M ,  LEWIS DAVID L

IPC分类号： A61K48/00 ,  A61K31/70 ,  A61K47/48 ,  C07H21/00 ,  C12N15/11 ,  C12N15/113 ,  C12N15/87

CPC分类号： A61K31/70 ,  A61K47/58 ,  A61K47/59 ,  A61K47/645 ,  A61K48/00 ,  A61K48/0075 ,  A61K48/0083 ,  C07H21/00 ,  C12N15/111 ,  C12N15/113 ,  C12N15/87 ,  C12N2310/11 ,  C12N2310/14 ,  C12N2310/3233 ,  C12N2320/32 ,  A61K2300/00

摘要： Disclosed is a process for delivering a polynucleotide to a mammalian cell to alter endogenous properties of the cell. The process comprises designing a polynucleotide, such as an siRNA, for transfection. Then the polynucleotide is inserted into a mammalian vessel such as an artery. Prior to insertion, subsequent to insertion, or concurrent with insertion the permeability of the vessel is increased thereby the polynucleotide is delivered to the parenchymal cell altering endogenous properties of the cell.