公开(公告)号：EP2576762A2

公开(公告)日：2013-04-10

申请号：EP11790526.5

申请日：2011-06-03

申请人： Mascoma Corporation ,  Stellenbosch University

发明人： BREVNOVA, Elena ,  MCBRIDE, John, E. ,  WISWALL, Erin ,  WENGER, Kevin, S. ,  CAIAZZA, Nicky ,  HAU, Heidi, H. ,  ARGYROS, Aaron ,  AGBOGBO, Frank ,  RICE, Charles, F. ,  BARRETT, Trisha ,  BARDSLEY, John, S. ,  FOSTER, Abigail, S. ,  WARNER, Anne, K. ,  MELLON, Mark ,  SKINNER, Ryan ,  SHIKHARE, Indraneel ,  DEN HAAN, Riaan ,  GANDHI, Chhayal, V. ,  BELCHER, Alan ,  RAJGARHIA, Vineet, B. ,  FROEHLICH, Allan, C. ,  DELEAULT, Kristen, M. ,  STONEHOUSE, Emily ,  TRIPATHI, Shital, A. ,  GOSSELIN, Jennifer ,  CHIU, Yin-Ying ,  XU, Haowen

IPC分类号： C12N1/15 ,  C12N1/19

CPC分类号： C12N15/52 ,  C12N9/2405 ,  C12N9/2437 ,  C12N9/2445 ,  C12N9/248 ,  C12N9/2482 ,  C12N15/81 ,  C12P7/06 ,  C12Y302/01004 ,  C12Y302/01008 ,  C12Y302/01021 ,  C12Y302/01091 ,  Y02E50/17

摘要： The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured togetherand used to produce ethanol from biomass feedstock.

公开(公告)号：EP2361311A2

公开(公告)日：2011-08-31

申请号：EP09828362.5

申请日：2009-11-23

申请人： Mascoma Corporation

发明人： MCBRIDE, John ,  BREVNOVA, Elena ,  GHANDI, Chhayal ,  MELLON, Mark ,  FROEHLICH, Alan ,  DELEAULT, Kristen ,  RAJGHARIA, Vineet ,  FLATT, Jim ,  VAN ZYL, Emile ,  DEN HAAN, Riaan ,  LAGRANGE, Danie ,  ROSE, Shaunita ,  PENTTILA, Merja ,  ILMEN, Marja ,  SIIKA-AHO, Matti ,  UUSITALO, Jaana ,  HAU, Heidi Hanson ,  RICE, Charles ,  VILLARI, Jeff ,  STONEHOUSE, Emily A. ,  GILBERT, Alan ,  KEATING, Jeffrey D. ,  XU, Haowen ,  WILLES, Deidre ,  SHIKHARE, Indraneel ,  THORNGREN, Naomi ,  WARNER, Anne K. ,  MURPHY, Dan

IPC分类号： C12P7/06

CPC分类号： C12P7/10 ,  C12N9/2437 ,  C12N9/2445 ,  C12N15/80 ,  C12P7/14 ,  C12P39/00 ,  C12Y302/01004 ,  C12Y302/01021 ,  C12Y302/01091 ,  Y02E50/16 ,  Y02E50/17

摘要： Host cells, comprising Kluveryomyces expressing heterologous cellulases produce ethanol from cellulose In addition, multiple host cells expressing different heterlogous cellulases can be co-cultured together and used to produce ethanol from cellulose The recombinant yeast strains and co-cultures of the yeast strains can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of sacchanfication and fermentation processes