公开(公告)号：EP2576762A2

公开(公告)日：2013-04-10

申请号：EP11790526.5

申请日：2011-06-03

申请人： Mascoma Corporation ,  Stellenbosch University

发明人： BREVNOVA, Elena ,  MCBRIDE, John, E. ,  WISWALL, Erin ,  WENGER, Kevin, S. ,  CAIAZZA, Nicky ,  HAU, Heidi, H. ,  ARGYROS, Aaron ,  AGBOGBO, Frank ,  RICE, Charles, F. ,  BARRETT, Trisha ,  BARDSLEY, John, S. ,  FOSTER, Abigail, S. ,  WARNER, Anne, K. ,  MELLON, Mark ,  SKINNER, Ryan ,  SHIKHARE, Indraneel ,  DEN HAAN, Riaan ,  GANDHI, Chhayal, V. ,  BELCHER, Alan ,  RAJGARHIA, Vineet, B. ,  FROEHLICH, Allan, C. ,  DELEAULT, Kristen, M. ,  STONEHOUSE, Emily ,  TRIPATHI, Shital, A. ,  GOSSELIN, Jennifer ,  CHIU, Yin-Ying ,  XU, Haowen

IPC分类号： C12N1/15 ,  C12N1/19

CPC分类号： C12N15/52 ,  C12N9/2405 ,  C12N9/2437 ,  C12N9/2445 ,  C12N9/248 ,  C12N9/2482 ,  C12N15/81 ,  C12P7/06 ,  C12Y302/01004 ,  C12Y302/01008 ,  C12Y302/01021 ,  C12Y302/01091 ,  Y02E50/17

摘要： The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured togetherand used to produce ethanol from biomass feedstock.

公开(公告)号：EP2467490A1

公开(公告)日：2012-06-27

申请号：EP10748027.9

申请日：2010-08-20

申请人： Mascoma Corporation

发明人： MCBRIDE, John ,  RAJGARHIA, Vineet ,  SHAW, Arthur J., IV ,  TRIPATHI, Shital, A. ,  BREVNOVA, Elena ,  CAIAZZA, Nicky ,  VAN DIJKEN, Hans ,  FROEHLICH, Allan ,  SILLERS, William, Ryan ,  FLATT, Jim

IPC分类号： C12P7/00 ,  C12P7/08 ,  C12P7/20 ,  C12N15/52

CPC分类号： C12P7/18 ,  C12P7/04 ,  C12P7/36 ,  C12P2203/00

摘要： The present invention provides for novel metabolic pathways leading to propanol, alcohol or polyol formation in a consolidated bioprocessing system (CBP), where lignocellulosic biomass is efficiently converted to such products. More specifically, the invention provides for a recombinant microorganism, where the microorganism expresses one or more native and/or heterologous enzymes; where the one or more enzymes function in one or more engineered metabolic pathways to achieve: (1) conversion of a carbohydrate source to 1,2-propanediol, isopropropanol, ethanol and/or glycerol; (2) conversion of a carbohydrate source to n-propanol and isopropanol; (3) conversion of a carbohydrate source to isopropanol and methanol; or (4) conversion of a carbohydrate source to propanediol and acetone; wherein the one or more native and/or heterologous enzymes is activated, up-regulated or down-regulated.

摘要翻译： 本发明提供了在固体生物处理系统（CBP）中导致丙醇，醇或多元醇形成的新型代谢途径，其中木质纤维素生物质被有效地转化为这样的产物。 更具体地，本发明提供重组微生物，其中微生物表达一种或多种天然和/或异源酶; 其中一种或多种酶在一个或多个工程化代谢途径中起作用以实现：（1）将碳水化合物源转化为1,2-丙二醇，异丙醇，乙醇和/或甘油; （2）将碳水化合物源转化成正丙醇和异丙醇; （3）将碳水化合物源转化成异丙醇和甲醇; 或（4）将碳水化合物源转化成丙二醇和丙酮; 其中所述一种或多种天然和/或异源酶被激活，上调或下调。

公开(公告)号：EP3168300A1

公开(公告)日：2017-05-17

申请号：EP16192843.7

申请日：2011-06-03

申请人： Lallemand Hungary Liquidity Management LLC ,  Stellenbosch University

发明人： BREVNOVA, Elena ,  MCBRIDE, John, E. ,  WISWALL, Erin ,  WENGER, Kevin, S. ,  CAIAZZA, Nicky ,  HAU, Heidi, H. ,  ARGYROS, Aaron ,  AGBOGBO, Frank ,  RICE, Charles, F. ,  BARRETT, Trisha ,  BARDSLEY, John, S. ,  FOSTER, Abigail, S. ,  WARNER, Anne, K. ,  MELLON, Mark ,  SKINNER, Ryan ,  SHIKHARE, Indraneel ,  DEN HAAN, Riaan ,  GANDHI, Chhayal, V. ,  BELCHER, Alan ,  RAJGARHIA, Vineet, B. ,  FROEHLICH, Allan, C. ,  DELEAULT, Kristen, M. ,  STONEHOUSE, Emily ,  TRIPATHI, Shital, A. ,  GOSSELIN, Jennifer ,  CHIU, Yin-Ying ,  XU, Haowen

IPC分类号： C12N9/42 ,  C12P7/06

CPC分类号： C12N15/52 ,  C12N9/2405 ,  C12N9/2437 ,  C12N9/2445 ,  C12N9/248 ,  C12N9/2482 ,  C12N15/81 ,  C12P7/06 ,  C12Y302/01004 ,  C12Y302/01008 ,  C12Y302/01021 ,  C12Y302/01091 ,  Y02E50/17

摘要： The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured togetherand used to produce ethanol from biomass feedstock.

摘要翻译： 本发明涉及一种或多种酵母菌株，其分泌全套或全套的任何子集的酶以水解玉米淀粉，玉米纤维，木质纤维素（包括水解纤维素，半纤维素和 在木质素和碳水化合物之间）并利用戊糖（木糖和阿拉伯糖）。 本发明还涉及用于每种酶活性类别的在酵母中良好表达的一组蛋白质。 所得菌株或菌株可用于同时水解淀粉和纤维素。 得到的菌株或菌株也可以被代谢工程化以产生更少的甘油并摄取乙酸盐。 所得到的菌株或菌株也可用于从粒状淀粉生产乙醇而不液化。 得到的一种或多种菌株可以进一步用于减少在同时糖化和发酵（SSF）过程中水解生物质原料所需的外部酶的量，或者在当前的糖分解酶载量下在SSF期间增加乙醇的产量。 另外，本发明的多种酶可以在本发明的细胞中共表达以提供对生物质原料的协同消化作用。 在一些方面，表达不同异源糖分解酶的宿主细胞也可以共培养以用于从生物质原料生产乙醇。