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1.发明公开YEAST EXPRESSING SACCHAROLYTIC ENZYMES FOR CONSOLIDATED BIOPROCESSING USING STARCH AND CELLULOSE 审中-公开酵母表达SACCHAROLYTISCHER酶联合生物利用强度和纤维素
公开(公告)号：EP2576762A2
公开(公告)日：2013-04-10
申请号：EP11790526.5
申请日：2011-06-03
发明人： BREVNOVA, Elena , MCBRIDE, John, E. , WISWALL, Erin , WENGER, Kevin, S. , CAIAZZA, Nicky , HAU, Heidi, H. , ARGYROS, Aaron , AGBOGBO, Frank , RICE, Charles, F. , BARRETT, Trisha , BARDSLEY, John, S. , FOSTER, Abigail, S. , WARNER, Anne, K. , MELLON, Mark , SKINNER, Ryan , SHIKHARE, Indraneel , DEN HAAN, Riaan , GANDHI, Chhayal, V. , BELCHER, Alan , RAJGARHIA, Vineet, B. , FROEHLICH, Allan, C. , DELEAULT, Kristen, M. , STONEHOUSE, Emily , TRIPATHI, Shital, A. , GOSSELIN, Jennifer , CHIU, Yin-Ying , XU, Haowen
CPC分类号： C12N15/52 , C12N9/2405 , C12N9/2437 , C12N9/2445 , C12N9/248 , C12N9/2482 , C12N15/81 , C12P7/06 , C12Y302/01004 , C12Y302/01008 , C12Y302/01021 , C12Y302/01091 , Y02E50/17
摘要： The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured togetherand used to produce ethanol from biomass feedstock.
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2.发明授权YEAST EXPRESSING CELLULASES FOR SIMULTANEOUS SACCHARIFICATION AND FERMENTATION USING CELLULOSE 有权HEFEEXPRIMIERENDE CELLULASENFÜRSIMULTANE VERZUCKERUNG UNDGÄRUNGMITTELS CELLULSE
公开(公告)号：EP2361311B1
公开(公告)日：2017-01-18
申请号：EP09828362.5
申请日：2009-11-23
发明人： MCBRIDE, John , BREVNOVA, Elena , GHANDI, Chhayal , MELLON, Mark , FROEHLICH, Alan , DELEAULT, Kristen , RAJGARHIA, Vineet , FLATT, Jim , VAN ZYL, Emile , DEN HAAN, Riaan , LAGRANGE, Danie , ROSE, Shaunita , PENTTILA, Merja , ILMEN, Marja , SIIKA-AHO, Matti , UUSITALO, Jaana , HAU, Heidi Hanson , RICE, Charles , VILLARI, Jeff , STONEHOUSE, Emily A. , GILBERT, Alan , KEATING, Jeffrey D. , XU, Haowen , WILLES, Deidre , SHIKHARE, Indraneel , THORNGREN, Naomi , WARNER, Anne K. , MURPHY, Dan
CPC分类号： C12P7/10 , C12N9/2437 , C12N9/2445 , C12N15/80 , C12P7/14 , C12P39/00 , C12Y302/01004 , C12Y302/01021 , C12Y302/01091 , Y02E50/16 , Y02E50/17
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3.发明公开YEAST EXPRESSING SACCHAROLYTIC ENZYMES FOR CONSOLIDATED BIOPROCESSING USING STARCH AND CELLULOSE 审中-公开酵母表达淀粉和纤维素用于固结生物加工的糖化酶
公开(公告)号：EP3168300A1
公开(公告)日：2017-05-17
申请号：EP16192843.7
申请日：2011-06-03
发明人： BREVNOVA, Elena , MCBRIDE, John, E. , WISWALL, Erin , WENGER, Kevin, S. , CAIAZZA, Nicky , HAU, Heidi, H. , ARGYROS, Aaron , AGBOGBO, Frank , RICE, Charles, F. , BARRETT, Trisha , BARDSLEY, John, S. , FOSTER, Abigail, S. , WARNER, Anne, K. , MELLON, Mark , SKINNER, Ryan , SHIKHARE, Indraneel , DEN HAAN, Riaan , GANDHI, Chhayal, V. , BELCHER, Alan , RAJGARHIA, Vineet, B. , FROEHLICH, Allan, C. , DELEAULT, Kristen, M. , STONEHOUSE, Emily , TRIPATHI, Shital, A. , GOSSELIN, Jennifer , CHIU, Yin-Ying , XU, Haowen
CPC分类号： C12N15/52 , C12N9/2405 , C12N9/2437 , C12N9/2445 , C12N9/248 , C12N9/2482 , C12N15/81 , C12P7/06 , C12Y302/01004 , C12Y302/01008 , C12Y302/01021 , C12Y302/01091 , Y02E50/17
摘要： The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured togetherand used to produce ethanol from biomass feedstock.
摘要翻译： 本发明涉及一种或多种酵母菌株，其分泌全套或全套的任何子集的酶以水解玉米淀粉，玉米纤维，木质纤维素（包括水解纤维素，半纤维素和 在木质素和碳水化合物之间）并利用戊糖（木糖和阿拉伯糖）。 本发明还涉及用于每种酶活性类别的在酵母中良好表达的一组蛋白质。 所得菌株或菌株可用于同时水解淀粉和纤维素。 得到的菌株或菌株也可以被代谢工程化以产生更少的甘油并摄取乙酸盐。 所得到的菌株或菌株也可用于从粒状淀粉生产乙醇而不液化。 得到的一种或多种菌株可以进一步用于减少在同时糖化和发酵（SSF）过程中水解生物质原料所需的外部酶的量，或者在当前的糖分解酶载量下在SSF期间增加乙醇的产量。 另外，本发明的多种酶可以在本发明的细胞中共表达以提供对生物质原料的协同消化作用。 在一些方面，表达不同异源糖分解酶的宿主细胞也可以共培养以用于从生物质原料生产乙醇。
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4.发明公开YEAST EXPRESSING CELLULASES FOR SIMULTANEOUS SACCHARIFICATION AND FERMENTATION USING CELLULOSE 有权HEFEEXPRIMIERENDE纤维素酶用于糖化和皮肤BY CELLULSE
公开(公告)号：EP2361311A2
公开(公告)日：2011-08-31
申请号：EP09828362.5
申请日：2009-11-23
申请人： Mascoma Corporation
发明人： MCBRIDE, John , BREVNOVA, Elena , GHANDI, Chhayal , MELLON, Mark , FROEHLICH, Alan , DELEAULT, Kristen , RAJGHARIA, Vineet , FLATT, Jim , VAN ZYL, Emile , DEN HAAN, Riaan , LAGRANGE, Danie , ROSE, Shaunita , PENTTILA, Merja , ILMEN, Marja , SIIKA-AHO, Matti , UUSITALO, Jaana , HAU, Heidi Hanson , RICE, Charles , VILLARI, Jeff , STONEHOUSE, Emily A. , GILBERT, Alan , KEATING, Jeffrey D. , XU, Haowen , WILLES, Deidre , SHIKHARE, Indraneel , THORNGREN, Naomi , WARNER, Anne K. , MURPHY, Dan
IPC分类号： C12P7/06
CPC分类号： C12P7/10 , C12N9/2437 , C12N9/2445 , C12N15/80 , C12P7/14 , C12P39/00 , C12Y302/01004 , C12Y302/01021 , C12Y302/01091 , Y02E50/16 , Y02E50/17
摘要： Host cells, comprising Kluveryomyces expressing heterologous cellulases produce ethanol from cellulose In addition, multiple host cells expressing different heterlogous cellulases can be co-cultured together and used to produce ethanol from cellulose The recombinant yeast strains and co-cultures of the yeast strains can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of sacchanfication and fermentation processes
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