公开(公告)号：EP2064225A2

公开(公告)日：2009-06-03

申请号：EP08769395.8

申请日：2008-05-09

申请人： Mascoma Corporation

发明人： HOGSETT, David, A. ,  RAJGARHIA, Vineet, B.

IPC分类号： C07H21/02 ,  C12N1/20 ,  C12N15/00

CPC分类号： C12P7/10 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/1205 ,  C12N9/1217 ,  C12N9/88 ,  C12N9/90 ,  C12N9/92 ,  C12N15/74 ,  C12P7/065 ,  C12P2203/00 ,  C12Y101/01002 ,  C12Y101/01027 ,  C12Y101/02003 ,  C12Y203/01008 ,  C12Y207/01017 ,  C12Y207/02001 ,  C12Y503/01005 ,  Y02E50/16 ,  Y02E50/17

摘要： One aspect of the invention relates to a genetically modified thermophilic or mesophilic microorganism, wherein a first native gene is partially, substantially, or completely deleted, silenced, inactivated, or down-regulated, which first native gene encodes a first native enzyme involved in the metabolic production of an organic acid or a salt thereof, thereby increasing the native ability of said thermophilic or mesophilic microorganism to produce ethanol as a fermentation product. In certain embodiments, the aforementioned microorganism further comprises a first non-native gene, which first non- native gene encodes a first non-native enzyme involved in the metabolic production of ethanol. Another aspect of the invention relates to a process for converting lignocellulosic biomass to ethanol, comprising contacting lignocellulosic biomass with a genetically modified thermophilic or mesophilic microorganism.

公开(公告)号：EP2576762A2

公开(公告)日：2013-04-10

申请号：EP11790526.5

申请日：2011-06-03

申请人： Mascoma Corporation ,  Stellenbosch University

发明人： BREVNOVA, Elena ,  MCBRIDE, John, E. ,  WISWALL, Erin ,  WENGER, Kevin, S. ,  CAIAZZA, Nicky ,  HAU, Heidi, H. ,  ARGYROS, Aaron ,  AGBOGBO, Frank ,  RICE, Charles, F. ,  BARRETT, Trisha ,  BARDSLEY, John, S. ,  FOSTER, Abigail, S. ,  WARNER, Anne, K. ,  MELLON, Mark ,  SKINNER, Ryan ,  SHIKHARE, Indraneel ,  DEN HAAN, Riaan ,  GANDHI, Chhayal, V. ,  BELCHER, Alan ,  RAJGARHIA, Vineet, B. ,  FROEHLICH, Allan, C. ,  DELEAULT, Kristen, M. ,  STONEHOUSE, Emily ,  TRIPATHI, Shital, A. ,  GOSSELIN, Jennifer ,  CHIU, Yin-Ying ,  XU, Haowen

IPC分类号： C12N1/15 ,  C12N1/19

CPC分类号： C12N15/52 ,  C12N9/2405 ,  C12N9/2437 ,  C12N9/2445 ,  C12N9/248 ,  C12N9/2482 ,  C12N15/81 ,  C12P7/06 ,  C12Y302/01004 ,  C12Y302/01008 ,  C12Y302/01021 ,  C12Y302/01091 ,  Y02E50/17

摘要： The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured togetherand used to produce ethanol from biomass feedstock.