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    SYNTHETIC NUCLEIC ACIDS FOR POLYMERIZATION REACTIONS
    4.
    发明公开
    SYNTHETIC NUCLEIC ACIDS FOR POLYMERIZATION REACTIONS 有权
    SYNTHETISCHENUKLEINSÄURENFÜRPOLYMERISATIONSREAKINGEN

    公开(公告)号：EP2836603A4

    公开(公告)日：2016-06-29

    申请号：EP13775998

    申请日：2013-04-04

    申请人： NEW ENGLAND BIOLABS INC

    发明人： JOHNSON DONALD ,  EVANS THOMAS C ,  GREENOUGH LUCIA ,  ONG JENNIFER

    IPC分类号： C12P19/34

    CPC分类号： C12Q1/6848 ,  C12N9/1252 ,  C12Q2525/101 ,  C12Q2525/301 ,  C12Q2527/127 ,  C12Q2549/101

    摘要： Compositions and methods are provided for inhibiting a polymerase from replicating non target DNA at a temperature below the amplification reaction temperature. The inhibitor is a synthetic nucleic acid which is single stranded but folds to form at least one double stranded region designed to melt at a temperature which is lower than the amplification reaction temperature, and at least one single stranded region where the single stranded region at the 5' end contains at least one uracil or inosine and optionally a sequence at the 3' end contains one or more derivative nucleotide or linkages.

    摘要翻译： 提供的组合物和方法用于在低于扩增反应温度的温度下抑制聚合酶复制非靶DNA。 抑制剂是单链的合成核酸，但折叠形成设计成在低于扩增反应温度的温度下熔化的至少一个双链区域，以及至少一个单链区域，其中单链区域 5'末端含有至少一个尿嘧啶或肌苷，任选的3'端的序列含有一个或多个衍生的核苷酸或连接。

    INTEIN MEDIATED PEPTIDE LIGATION
    7.
    发明公开
    INTEIN MEDIATED PEPTIDE LIGATION 有权
    内含肽介导肽连接

    公开(公告)号：EP1117693A4

    公开(公告)日：2003-06-25

    申请号：EP99950063

    申请日：1999-09-30

    申请人： NEW ENGLAND BIOLABS INC

    发明人： XU MING-QUN ,  EVANS THOMAS C

    IPC分类号： C12N15/09 ,  C07K1/04 ,  C07K1/107 ,  C07K19/00 ,  C12N9/00 ,  C12N15/62 ,  C12N9/90 ,  C12P21/02 ,  C12P21/04

    CPC分类号： C12N9/93 ,  C07K2319/20 ,  C07K2319/24 ,  C07K2319/55 ,  C07K2319/92 ,  C12N15/62

    摘要： An in vitro method for producing a semi-synthetic fusion protein is provided, whereby a target protein fused to an intein - a protein splicing element - is selectively cleaved in a first step as depicted in Figure 1 with a thiol reagent, forming a carboxyl-terminal thioester of the target protein and releasing the target protein from the intein. In a subsequent step as shown in Figure 1, a desired, synthetic, protein or peptide having an amino-terminal cysteine is ligated to the target protein. Standard thiol-reagents such as DTT, or thiol-reagents optimized for ligation such as the odorless MESNA, may be used in the first step. The method permits the direct ligation of a desired peptide to a thioester bond that had linked a target protein to an intein. An in vivo variation of the method will permit production of a cytotoxic protein: a truncated, inactive, form of the protein fused to an intein is introduced in vivo, this fusion product is then selectively cleaved, and a synthetic protein or peptide is subsequently ligated at a carboxyl-terminal thioester of the target protein in order to restore the native activity of the cytotoxic protein.