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公开(公告)号：EP2395358B1
公开(公告)日：2017-03-29
申请号：EP11003760.3
申请日：2005-07-29
申请人： Promega Corporation
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公开(公告)号：EP2327768B1
公开(公告)日：2015-09-09
申请号：EP11155576.9
申请日：2007-04-02
申请人： Promega Corporation
发明人： Binkowski, Brock , Fan, Frank , Wigdal, Susan , Wood, Keith V. , Wood, Monika G.
CPC分类号： C12N9/0069 , C12N9/0004 , C12Q1/66 , C12Y113/12007 , G01N33/84
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3.发明公开
公开(公告)号：EP2860252A1
公开(公告)日：2015-04-15
申请号：EP14196073.2
申请日：1998-09-18
申请人： PROMEGA CORPORATION
发明人： Wood, Keith V. , Hall, Mary P.
CPC分类号： C12N9/0069 , C12N15/10
摘要： Luciferase enzymes with greatly increased thermostability, e.g., at least half lives of 2 hours at 50°C, cDNAs encoding the novel luciferases, and hosts transformed to express the lucifases, are disclosed. Methods of producing the luciferases include recursive mutagenesis. The luciferases are used in conventional methods, some employing kits.
摘要翻译： 公开了具有大大增加的热稳定性的荧光素酶，例如在50℃下2小时的至少半衰期，编码新的荧光素酶的cDNA和转化以表达lucifases的宿主。 产生荧光素酶的方法包括递归诱变。 荧光素酶用于常规方法，一些采用试剂盒。
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公开(公告)号：EP2341134A3
公开(公告)日：2011-08-31
申请号：EP10075510.7
申请日：2004-01-30
申请人： Promega Corporation
发明人： Wood, Keith V. , Los, Georgyi V. , Bulleit, Robert F. , Klaubert, Dieter , McDougall, Mark , Zimprich, Chad
CPC分类号： C12Q1/34 , C07C217/08 , C07C271/16 , C07D209/48 , C07D233/64 , C07D311/78 , C07D311/82 , C07D405/06 , C07D493/10 , C07D495/04 , C07F5/022 , C12N9/14 , C12N9/86 , C12Y308/01005 , G01N33/5005 , G01N33/58 , Y10S436/80
摘要： A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.
摘要翻译： 提供了任选地与感兴趣的蛋白质融合的突变水解酶。 突变水解酶能够与相应的非突变型（野生型）水解酶的底物形成键，其比野生型水解酶和底物之间形成的键更稳定。 还提供了包含一个或多个官能团的用于水解酶的底物，以及使用突变水解酶和本发明底物的方法。 还提供了能够与底物和表达融合蛋白的细胞形成稳定键的融合蛋白。
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5.发明公开Luminogenic and fluorogenic compounds and methods to detect molecules or conditions 有权发光和荧光化合物和检测分子或条件的方法
公开(公告)号：EP2272973A1
公开(公告)日：2011-01-12
申请号：EP10075501.6
申请日：2006-05-31
申请人： Promega Corporation
发明人： Cali, James J. , Daily, William , Hawkins, Erica , Klaubert, Dieter , Liu, Jianquan , Meisenheimer, Poncho , Scurria, Michael , Shultz, John W. , Unch, James , Wood, Keith V. , Zhou, Wenhui , Valley, Michael P.
IPC分类号： C12Q1/66 , C07D277/66
CPC分类号： C07D277/66 , C07D277/68 , C07D311/16 , C07D417/04 , C07D417/14 , C07D493/10 , C07F9/65583 , C09B11/24 , C09B19/00 , C09B57/00 , C09B57/02 , C12Q1/66 , G01N2500/00
摘要： A method to detect the presence or amount of at least one molecule in a sample which employs a derivative of luciferin or a derivative of a fluorophore is provided.
摘要翻译： 提供了检测采用荧光素衍生物或荧光团衍生物的样品中至少一种分子的存在或量的方法。
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6.发明公开Luminogenic and fluorogenic compounds and methods to detect molecules or conditions 审中-公开和用于检测分子或痛苦的发光的，荧光化合物和方法
公开(公告)号：EP2272972A1
公开(公告)日：2011-01-12
申请号：EP10075500.8
申请日：2006-05-31
申请人： Promega Corporation
发明人： Cali, James J. , Daily, William , Hawkins, Erica , Klaubert, Dieter , Liu, Jianquan , Meisenheimer, Poncho , Scurria, Michael , Shultz, John W. , Unch, James , Wood, Keith V. , Zhou, Wenhui , Valley, Michael P.
IPC分类号： C12Q1/66 , C07D277/66
CPC分类号： C07D277/66 , C07D277/68 , C07D311/16 , C07D417/04 , C07D417/14 , C07D493/10 , C07F9/65583 , C09B11/24 , C09B19/00 , C09B57/00 , C09B57/02 , C12Q1/66 , G01N2500/00
摘要： A method to detect the presence or amount of at least one molecule in a sample which employs a derivative of luciferin or a derivative of a fluorophore is provided.
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公开(公告)号：EP3409764B1
公开(公告)日：2020-04-15
申请号：EP18184699.9
申请日：2010-05-03
申请人： Promega Corporation
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公开(公告)号：EP3409764A1
公开(公告)日：2018-12-05
申请号：EP18184699.9
申请日：2010-05-03
申请人： Promega Corporation
发明人： Encell, Lance P. , Wood, Keith V. , Wood, Monika G. , Hall, Mary P. , Otto, Paul , Vidugiris, Gediminas , Zimmerman, Kristopher
摘要： The present invention discloses a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO: 1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.
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公开(公告)号：EP2308978B8
公开(公告)日：2015-04-08
申请号：EP10182746.7
申请日：2004-10-01
申请人： Promega Corporation
IPC分类号： C12N15/53 , C12N9/02 , C12Q1/66 , G01N33/573
CPC分类号： C12N9/0069 , C07K2319/20 , C07K2319/21 , C07K2319/23 , C12Q1/66 , G01N2500/04
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公开(公告)号：EP2341134B1
公开(公告)日：2014-08-27
申请号：EP10075510.7
申请日：2004-01-30
申请人： Promega Corporation
发明人： Wood, Keith V. , Los, Georgyi V. , Bulleit, Robert F. , Klaubert, Dieter , McDougall, Mark , Zimprich, Chad
CPC分类号： C12Q1/34 , C07C217/08 , C07C271/16 , C07D209/48 , C07D233/64 , C07D311/78 , C07D311/82 , C07D405/06 , C07D493/10 , C07D495/04 , C07F5/022 , C12N9/14 , C12N9/86 , C12Y308/01005 , G01N33/5005 , G01N33/58 , Y10S436/80
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